隆相 的英文怎麼說
中文拼音 [lōngxiāng]
隆相
英文
takasuke-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。The chs gene which is obvious correlated with anthocyanin accumulation was cloned as well
對與花青素積累有明顯相關的chs基因進行了克隆。Compared with the known antibodies, the antiserum we prepared is highly specific and rules out the possibility of cross - reacting with angiotensin fragments. 4
與己有抗體相比，本實驗制備的抗agt多克隆抗血清不僅具有較高的特異性，而且排除了與血管緊張素短片段的交叉反應。A rabbit was infected with a cloned yntatl, blood was collecting from from the rabbit every 3 days after infection within 30 days, 10 clonal trypanosome populations were gotten, infecting a new rabbit by the last non - cloned trypanosome population. repeated above all, thus infected 5 rabbits sequentially. twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia )
用伊氏錐蟲雲南水牛單克隆株yntat1感染兔，感染后30天內，每3天從兔血中分離錐蟲並單蟲克隆，最後一個未單蟲克隆的蟲株感染另一隻兔，重復以上操作，這樣順序感染5隻兔子，共獲得50個單克隆錐蟲種群（ tp ） ，經間接免疫熒光和abc酶標試驗鑒定共為20個抗原性互不相同的抗原變異體（ vats ） 。The impoverished african nation of burundi, by contrast, spends just $ 6 per patient
與之相比，在窮困的非洲國家蒲隆地，用於每位患者的費用僅為6美元。The catalan numbers also count the number of ways in which an n-gon can be dissected into triangles by nonintersecting diagonals.
加泰隆數還數計有多少方式來將多邊形用不相交的對角線劃分成三角形。Cloning of the cleft palate related gene in mouse and the significance of the gene expression
小鼠齶裂相關基因的克隆及基因表達的意義Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank
以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。Hong kong strongbase group, mainly invested by hong kong strongbase investment co., ltd., is a high - tech enterprise, which specialize in manufacturing, researching developing and promoting led, led related products, accessories and appliances. for the past twenty years, strong base group has been pursued its philosophy of professional management, serial development and scale management with " honesty, factualism, innovation " as its operation tenet
香港健隆集團是以香港健隆投資公司為投資主體，以led封裝及相關配套產品關聯產品應用產品的生產研發推廣應用為主的高科技企業集團，公司集科研開發生產銷售服務於一體，具有從原材料到最終產品一條生產能力，是目前最具規模的led科研生產基地。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method
鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function
進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。Molecular biology reagents of gene cloning 4
基因克隆相關分子生物學試劑4Browse charlize theron desktop gallery
瀏覽莎莉賽隆相片庫It still remains a question whether the rearrangements of igh come from h / rs cell or the background lymphocytes. in this study, we have detected the igh clonal correlation between the h / rs cells and the background cells, from a new aspect to study the clonality of h / rs cell and its relation with the background cells. the expression of b - cell - specific activator protein ( bsap ) was detected in hl. igh gene rearrangements were analysed by the methods including gene analysis in neoplasms tissue and micropicked cells from paraffin - embedded sections, sequencing to test the pcr product, and in situ pcr
本研究將在以往研究的基礎上，在國內率先把b細胞核反式作用因子? b細胞特異性激活蛋白（ b - cell - specificactivatorprotein ， bsap ）應用於hl的研究，檢測hl的bsap表達，並採用石蠟刮片組織和微切割單細胞的基因分析、測序分析和間接原位pcr等方法，同步觀察分析h rs和背景淋巴細胞的igh基因克隆相關性，從又一個新視角探究chl的腫瘤性h rs細胞克隆性及與背景淋巴細胞的關系。Hence, an elf like lindir of rivendell may seem less wise when compared to legolas or elrond, but is not really dense or ignorant
所以，象利文德爾的林德那樣的精靈，在才智上和萊格拉斯或埃爾隆相比可能有些遜色，但他並不是真的那麼愚蠢和無知。Ceu line 2 ) molecular biology reagents of gene cloning 3 ) reagents for northern blot and rt - pcr 4 ) cell transfection kit 5 ) reagents for kinase assay 2
肝癌bel7402細胞系和cos7細胞系2基因克隆相關分子生物學試劑3 northern雜交及rt - pcr相關試劑4In this dissertation we focused on the function of genes associated with anther and pollen development including map - based cloning of arabidopsis genes from male sterile mutants
本論文以正向遺傳學的方法研究花藥及花粉發育過程的基因功能，主要利用圖位克隆的方法從擬南芥雄性不育突變體中克隆相應的基因。If you can deal with arvin sloane -
如果你可以和阿文斯隆相處Name me anyone who cares as much as eva peron
請問誰可和伊娃貝隆相比分享友人