隆貝克 的英文怎麼說
中文拼音 [lōngbèikè]
隆貝克
英文
lombeck-
" breast ironing is an age - old practice in cameroon, as well as in many other countries in west and central africa, including chad, togo, benin, guinea - conakry, just to name a few, " said flavien ndonko, an anthropologist and local representative of german development agency gtz, which sponsored the survey
一位人類學家及贊助這項調查的德國當地發展局代表福雷門說：燙胸部在柯麥隆是項流傳已久的習俗，這項習俗同時也在許多西方國家跟中非，包含查德、多哥、貝南、幾內亞首都科那克里，都記載不多。Bevacizumab, a monoclonal antibody against vascular endothelial growth factor, has shown promising preclinical and clinical activity against metastatic colorectal cancer, particularly in combination with chemotherapy
背景：一種新型的抗血管內皮單克隆抗體貝伐單抗在基礎以及臨床研究中均對轉移性結腸癌表現出良好的治療效果，尤其在與其他化療藥物結合時效果更佳。Concatemer were constructed by polymerization with t4 dna polymerase and primer - templated pcr with high fidelity dna polymerase. one concatemer of 360bp, 9 copies of grb - ast3, were obtained from the first pair of primer and three ones of 515bp, 750bp, 792bp, from the second one
從第一對引物的拼接產物中克隆到一個360bp含有9個拷貝數的grb - ast _ 3的串聯體，從第二對引物的拼接產物中克隆到三個分別為515 、 750 、 792bp的grb - ast _ 3的串聯體。During the battle of the department of mysteries, bellatrix taunted harry and used the cruciatus curse on neville when she heard that he was a longbottom
在神秘事務司的遭遇戰中，貝拉特里克斯不僅嘲笑哈利無能，還用鉆心咒折磨了隆巴頓夫婦的兒子納威。An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。In this article we cloned two crucial gibberellins biosynthesis enzyme genes : ent - kaurene oxidase and 20 - oxidase, and we investigated their expression patterns. 1
本文克隆了赤霉素生物合成途徑中關鍵合成酶：貝殼杉烯氧化酶和20 -氧化酶，並研究了二者在果樹生長發育中的表達規律。We cloned two ent - kaurene oxidase homologues from the reverse transcriptional products of different strawberry tissue, the identity of these two homologues is 88. 2 %. it suggest us that it belongs to a gene family. 3
在豐香草莓的不同發育部位克隆到同源性為88 . 2的兩個貝殼杉烯氧化酶的基因片段，預示著此基因是個基因家族。We designed degenerate primers of consensus amino acid sequences of ent - kaurene oxdase, and use them to amply the middle segments of apple, strawberry and peach, and then we designed specific primers insides these middle segments to amplify 3 " termini, and 5 " termini. finally we got the full length of strawberry and 3 ' teminal of peach
利用保守區域的兼并引物克隆了平邑甜茶，豐香草莓和碧桃貝殼杉烯氧化酶基因的長為410bp的中間片段，以中間片段序列為線索又克隆到萆莓貝殼杉烯氧化酶的全長，和碧桃此酶的3 』端。Then daguenet added a rapid word or two about the rest. there was clarisse besnus, whom a lady had brought up from saint - aubin - sur - mer in the capacity of maid while the lady s husband had started her in quite another line. there was simonne cabiroche, the daughter of a furniture dealer in the faubourg saint - antoine, who had been educated in a large boarding school with a view to becoming a governess. finally there were maria blond and louise violaine and lea de horn, who had all shot up to woman s estate on the pavements of paris, not to mention tatan nene, who had herded cows in champagne till she was twenty
隨后，其餘女人的情況達蓋內就三言兩語地說一下：克拉利瑟貝尼，是被一個太太從海濱聖歐班帶來作女僕的，后來那個太太的丈夫把她送出來當了煙花女西蒙娜卡比羅什是聖安托萬郊區的一個傢具商的女兒，在一所很大的培養小學教員的寄宿學校里長大瑪麗亞布隆路易絲維奧萊納和萊婭德霍恩都是被迫走上巴黎街頭，淪為娼妓的。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing
豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。This past week i was asked to participate at remembrance service for dr. arnold beckman, one of the greatest men ever to live in orange county, california
上周我出席了一個隆重的喪禮，不是列根的喪禮，而是貝克曼的追思禮拜。多少人聽過貝克曼這名字？The hplap induced from the clone with two copies of hplap gene had higher enzyme activity. the clone with more or less copy was unfavourable to the hplap expression in p. pastoris
對部分克隆進行誘導表達發現只有2拷貝轉化子表達產物hplap活性最高，過高過低拷貝數均不利丁hplap的表達。In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia
並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。Real president ramon calderon said many times in the autumn that he wanted to extend beckham s contract
皇馬俱樂部總裁拉蒙卡爾德隆說，今年秋天他多次想與貝克漢姆續約。Real president ramon calderon said many times in the autumn that he wanted to extend beckham ' s contract
皇馬俱樂部總裁拉蒙卡爾德隆說，今年秋天他多次想與貝克漢姆續約。Swiss international valon behrami has impressed since joining lazio this season, but president claudio lotito has sparked a financial battle over his future
瑞士國腳維隆.貝赫拉米這個賽季加盟拉齊奧后的表現給人印象深刻，但主席克勞迪奧.洛蒂托正在為他的未來進行一場金元戰爭。Beckham denied that real madrid president ramon calderon is trying to cut his wages as part of a new contract
貝克漢姆否認皇馬的主席羅曼?科爾德隆試圖在新合同中削減他的工資。分享友人