隨機引物 的英文怎麼說

中文拼音 [suíjīyǐnwù]
隨機引物 英文
random primer
  • 隨 : Ⅰ動詞1 （跟; 跟隨） follow 2 （順從） comply with; adapt to 3 （任憑; 由著） let （sb do as he li...
  • 機 : machineengine
  • 引 : Ⅰ動詞1 （牽引; 拉） draw; stretch 2 （引導） lead; guide 3 （離開） leave 4 （伸著） stretch 5 （...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 隨機 : random stochasticrandom
  1. Rapd ( random amplified polymorphic dna ) technique was used to study the genetic structure and genetic differentiation of the natural population and the hatchery stock of chlamys farreri. amplifications with 20 10 - base primers under predetermined optimal reaction yielded 153 reproducible amplified fragments ranging from 200 to 3000bp. the mean proportions of polymorphic amplified fragments of natural population and the hatchery stock were 73. 2 % and 70. 6 % respectively, and the mean hetreozygosities of them were 0. 27 and 0. 26 respectively

    在對20個野生櫛孔扇貝和20個養殖櫛孔扇貝的基因組dna的檢測中， 20個隨機引物共擴增出153條清晰可分辨的dna片段，片段大小在200 3000bp之間，其中野生種群和養殖群體多態性片段分別為112和108條，多態位點比例分別為73 . 2 %和70 . 6 % ，雜合度分別為0 . 27和0 . 26 。
  2. Two hundred and seventy - four sites were detected by 15 10 - mer primers, out of which 262 were polymorphic ( 95. 6 % of the total loci detected ). all of the materials were clearly distinguished, of which fourteen samples have forty - five typical rapd bands. before the molecular taxonomy was introduced, people never doubted subgenus yulania ' s disrelation with subgenus magnolia and a close relation between subgenus yulania and michelia, as there is in both genera a sameness seen in their prolepsis and powder chambers dehiscing on the side

    3 、通過對玉蘭亞屬rapd擴增譜帶的分析，發現該類群具有很高的多態性：多態位點百分率高達95 . 6 ，產生的多態性帶數與所用引物數的比例為17 . 6 ， 15條隨機引物清楚地分開了31個種類，其中的14個種共有45條特徵性帶，在利用rapd標記已研究過的類群中，本類群多態性較高。
  3. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  4. 92 distinct bands including 87 polymorphic bands and 24 specific bands were amplified from the genome dna of gastrodia elata bl. by using these selected primers belong to groups of opb, opi, opn, op w. the rate of polymorphic bands and the rate of specific bands were 93. 5 % and 25. 0 % respectively. seven often samples excluding tl, t3 and t9 were identified by the 24 specific bands

    隨機引物opb - 5擴增出的特異性條帶最多，有4條，特異性條帶率達57 . 2 ，可以鑒定出m和t8兩個樣品；隨機引物opn - 10擴增出的9條帶中，有3條特異性的帶，可區分m ， t8 ， t2三個樣品。
  5. Three natural population of l. sagitta, add up to 62 individual, with 17 random primers, 128 polymorphic loci

    箭葉橐吾的三個自然居群，總計62個個體，使用17個隨機引物，共擴增出128個多態位點。
  6. From this result it can be concluded that the est isoenzyme was very sensitivity for the toxication of hg24 and can be used as a bioindicator of the environmental pollution. the same experiment had been done twice in 2003. scetion : from october in 2002 to october in 2003, by using random amplified polymorphic dna ( rapd ), the effects on ovary ' s dna under different concentration of mercury in freshwater crab ( sinopotamon yangtsekiense ) were detected for the first time

    汞對長江華溪蟹卵巢總dna的影響汞對長江華溪蟹（ sinopot胭onyangtsekiense ）細胞及分子水平的影響以體外暴露的方法，對經汞體外暴露一周長江華溪蟹的卵巢總dna ，採用隨機引物擴增多態性dna （ rapd ）技術對13條隨機引物的擴增圖譜用nei共享度公式進行分析。
  7. The main results are shown as below : 1. 80 individuals from 4 populations ( 20 from each population ) of argopecten irradians were analyzed by using 20 random rapd primers. 132 rapd bands ranging from 230 to 2800bp were recorded with an average of 6. 6 bands gained by per primer

    採用20條隨機引物對4個群體80個個體（每個群體20個個體）進行了rapd群體遺傳多樣性分析，共擴增出132個位點，片段大小在230 - 2800bp之間，平均每條引物的擴增帶數是6 . 6條。
  8. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  9. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  10. Five natural population of l. virgaurea, add up to 100 individual, with 17 random primers, 132 polymorphic loci

    黃帚橐吾的五個自然居群，總計100個個體，使用17個隨機引物，共擴增出132個多態位點。
  11. Among 54 arbitrary primers tested, only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae

    本研究經用54種隨機引物對57種蟋蟀的rapd擴增分析得出以下結論： 1 ）採用rapd技術，首次較系統地對中國蟋蟀總科7科26屬共57種蟋蟀進行了系統學研究。在所試用的54種隨機引物中篩選出可擴增出清晰可辨且穩定的rapd圖譜的引物分別為：蟋蟀科9種、嶺蟋科9種、樹蟋科12種、結蟋科12種、蛛蟋科13種、鐵蟋科13種、貌蟋科13種。
  12. Random amplified polymorphic dna rapd technique was applied to assess six

    應用隨機引物擴增多態性dna rapd方法，對我國水青網屬
  13. By sun, bo ( major : marine biology ) directed by professor zhang, guofan and dr. liu, xiao population genetics of four argopecten irradians cultured populations was analyzed by using random amplification polymorphism dna ( rapd ) techniques. and partial dna sequences of the internal transcribed spacers ( its ) from four representative samples ( one sample represent for each population ) were amplified by pcr and sequenced in order to stress the phylogenetic relationships of the four populations. besides, by rapd technique, two argopecten irradians families were studied to examine whether the hybridization experiments were successful and which family was more suitable to be materials in future genetic linkage map construction

    本文以海灣扇貝4個養殖群體為研究對象，採用隨機引物多態性dna （ rapd ）技術進行了群體遺傳學研究，並嘗試使用4個群體中代表個體的內轉錄間隔區（ its ）序列比對分析結果作為參考；另外，本文還運用rapd技術對人工異體交配的2個海灣扇貝家系的雜交成功率進行了分析，均獲得成功證實，並評估了兩個家系構建遺傳連鎖圖譜的潛力。
  14. The double chains of chil - 1 cdna were labeled with dig - 11 - dutp to produce probe. and the chil - 1 mrna secreation was studied in the tissues of chickens " brains infected with aev and that of normal chickens ". the results showed that the content of chil - 1 secreation in sick chickens " brain was higher than that of the normal chickens "

    用地高辛隨機引物法標記il - 1 cdna制備探針，原位雜交檢測禽腦脊髓炎病雞與正常雞腦組織中il - 1分泌情況，結果表明腦炎病雞腦中il - 1分泌量高於正常雞。
  15. The procedure of ddrt - pcr applicable for silver staining was optimized by adjusting the amount of several critical reagents, including total rna, anchor primer, arbitrary primer, cdna and dntp

    通過調整ddrt - pcr中總rna 、錨定引物、隨機引物、 cdna和dntp等關鍵試劑的用量，優化了適用於銀染檢測的ddrt - pcr方法。
  16. So it is very important to isolate and clone these genes in leymus multicaulis for utilizing in breeding. in this research, ddrt - pcr technique was used to study these genes and two common wheat - leymus multicaulis disomic addition lines were included

    採用3條錨定引物和8條隨機引物共計24種組合，獲得了6480條耐病材料cdna擴增條帶，其中91 . 8在不同處理條件下是非常一致的。
  17. Rapd technique was first used to analyze the phylogenetic relationships of 13 species or varieties in pueraria dc complex. seventy - two random primers were tested, among which 18 chosen primers generated clear polymorphic bands

    從72個隨機引物中篩選出了18個引物在13個不同的種和類型的葛資源中具有rapd多態性。
  18. Sixty 10 - oligonucleotide primers from opron inc. were used for polymorphic selection, with fifty four ( 90 % ) primers that produced polymorphic products. a total of 209 bands amplified from 20 primers were selected for rapd analysis

    對60個來自opron公司的十堿基隨機引物進行了多態性篩選，其中54個00能擴增出多態性譜帶。
  19. The results showed that the higher genetic diversity and the genetic differentiation existed in the population of reaumuria soongorica, which was composed of 7 subpopulations and 136 individuals, generated by 15 primers. 71 loci had been detected and 69 were polymorphic

    15條隨機引物對紅砂7個亞種群的136個個體進行擴增，共檢測71個位點，其中多態位點69個。
  20. Twelve primers selected from 100 random primers amplified 254 frangments in 14 species of cymbidium, in which 243 segments are polymorphic. the rate of polymorphism was 95. 7 %. and all the segments were ranging from 200bp to 2200bp

    從100個隨機引物中篩選出12個隨機引物，對蘭屬植物14種材料進行隨機擴增，共得到254條帶，多態性帶有243條，多態性百分率為95 . 7 。
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