Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis
二、研究論文1 、參照人sry基因hmg - box保守區的序列,設計一對兼并引物, pcr擴增了中華絨螯
蟹的sox基因,並對擴增產物進行了克隆和測序。結果在
雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ,其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ,顯示該基因在進化上具高度的保守性。
The crabs were dissected respectively after one week of treatment, and the ovaries were kept in 70 % ethanol with 50mmol / l edta
一定濃度范圍內的汞處理
雌蟹,譜帶差異與其它濃度處理組同正常織相比較大。
At courtship time, he waves it to attract females, often with a beckoning movement, or to fight rival males
求偶期間,雄
蟹會揮動大
蟹鉗,吸引
雌蟹注意,或擊退情敵。
As a result, the mature female mud crab can spawn, though the number of eggs laid is affected
結果表明精英的人工移植不會破壞成熟
雌蟹納精囊的內環境,其內精子具正常受精能力。
A fisherman fishing just east of the farallon islands spotted a female humpback whale entangled in a web of crab traps and lines
一天,一名在法拉隆群島東邊打魚的漁民發現一條
雌性座頭鯨被捕
蟹網纏住。
The transferring in unmated female may not spawning normally, but ovary development is accelerated and also molt
未交配雌青蟹精英的人工移植沒有正常產卵,但性腺得以促進發育,並能進行正常的生殖脫皮。