雜交分子 的英文怎麼說

中文拼音 [zájiāofēnzi]
雜交分子 英文
hybrid molecule
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  2. Ascospore analysis suggested that the fungus is homothallic but will preferentially outcross.

    從分析子囊孢子的結果看出，此菌能同親配合，但很易異型雜交。
  3. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  4. The synthesis methods of isobutyl acetate catalyzed by p - toluene sulfonic acid, amino sulfonic acid, strongly acidic cationic exchange resin, ferric chloride hexahydrate, cupric chloride bi - hydrate, stannic chloride pentahydrate, ammonium ferric sulfate dodecahydrate, sodium bisulfate monohydrate, potassium bisulfate, chitosan sulfate, sodium dihydrogen phosphate, rare earth metallic oxide, solid super acid, heteropoly acid and molecular sieve etal were reviewed

    摘要評述了對甲苯磺酸、氨基磺酸、強酸性陽離子交換樹脂、六水三氯化鐵、二水氯化銅、五水四氯化錫、十二水合硫酸鐵銨、一水硫酸氫鈉、硫酸氫鉀、殼聚糖硫酸鹽、磷酸二氫鈉、稀土金屬氧化物、固體超強酸、雜多酸和分子篩等催化劑催化合成乙酸異丁酯的方法。
  5. By using these antibodies, which were raised to immunoreact with total proteins of purified mitochondria from different organs of mung bean seedlings, we find that there were two hybridizable aox bands in mitochondria in mung bean seedlings. their molecular weight was about 35kd and 38kd respectively

    將此抗體與來自綠豆幼苗不同器官線粒體的總蛋白分別進行western雜交，可觀察到綠豆幼苗線粒體中具有兩條清晰的aox雜交帶，它們的分子量分別為35kd和38kd 。
  6. The highest vt and p valt in true leaf were accompanied two hybridizable polypeptides of aox protein, 35kd and 38kd respectively. the next was cotyledon vt and p valt with only one 38kd hybridizable polypeptide of aox protein. hypocotyl vt and p valt were the lowest and its immunobloting band was similar to that of cotyledon, but the expressive amount of 38kd protein was less than that of cotyledon

    綠豆幼苗不同器官的有關呼吸參數測定結果與aox表達的western分析基本一致：真葉的v _ t特別是v _ （ alt ）最高，它也具有35kd和38kd的aox的雜交多肽；其次是子葉的v _ t和v _ （ alt ） ，且在子葉中，只見一條分子量為38kd的aox多肽；下胚軸的v _ t和v _ （ alt ）都最低， western雜交顯示也只有一條分子量為38kd的多肽，而且表達量也較少。
  7. The application of molecular hybridization in typing of human papilloma virus

    分子雜交技術在人乳頭瘤病毒分型檢測中的應用
  8. 2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin

    等（ 1995 ）利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種， allonemobiusfasciatus和a . socius ，並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖；國內田英芳、鄭哲民（ 20of ）首次將rapd技術運用於蟋蟀總科的分子系統學研究中，採用2種引物對7種蟋蟀進行了基困組dna多態性研究，並應用upg問a法構建樹狀圖，椎測系統發生關系
  9. Pupal hemolymph infected with recombinant virus bmpak - hbmp and bmpak - hbm showed four protein bands of about 31, ' 30, 28 and 25 kd in the western blotting profiles, due to the two atg initial codons at the 5 " ends of both pres2 and s gene

    Western雜交結果表明，感染bmpak hbmp和bmpak hbm的家蠶蛹血淋巴中均有4種不同分子量的蛋白質和鼠抗人的一抗發生免疫反應，大小約分別為31kd 、 30kd 、 zskd 、 25kd 。
  10. Salt treatment had effects on growth, succulence and some physiological parameters. in present study, suaeda salsa seedlings were treated with different salts and isoosmotic peg to examine the succulence and some physiological parameters. the hydraulic conductance ( lo ) of the roots, the water permeability of protoplasts and western blot analysis of aquaporins in plasma membrane and tonoplast under nacl were determined

    本實驗以鹽生植物堿蓬幼苗為材料，用不同的鹽及與nacl等滲的peg處理，測定肉質化及有關生理指標，並測定nacl處理下植物根的導水性，原生質體的水滲透性，並在分子水平上進行了細胞質膜及液泡膜水孔蛋白免疫雜交分析。
  11. Frankfurt os, robb ja, sugar baker ev, et al. monoclonal antibody tosingle2stranded dna is specific and sensitive cellular marker of apoptosis [ j. exp cell res, 1996, 226 ( 2 ) : 3872397

    吳國慶，李少華，徐志偉，等.抗單鏈單克隆抗體雜交瘤細胞株的建立及鑒定[ j ] .細胞與分子免疫學雜志， 2002 ， 18 （ 5 ） : 4792480
  12. Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process

    本論文在前人研究的基礎上，開展了以下兩個方面的工作： （ 1 ）採用分子生物學技術熒光原位雜交（ fish ） 、多聚酶鏈式反應（ pcr ） 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測； （ 2 ）探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性，為天然底質環境中厭氧氨氧化過程的強化，富營養化底質微生物修復的可行性提供一定的依據。
  13. Abstract : the paper considers that the experiment of affect scion of prunus japonica thunb on stock of prunus armeniaca l. furnish fresh evidence for graft hybridization ， and proposes the possible mechnism for production of tillering prunus japoniaca thunb according to recent achievements in plant molecular biology

    摘要本文認為「接穗郁李影響杏砧變異的實驗」為嫁接雜交研究提供了新的證據，並根據近年來植物分子生物學的有關研究成果，對蘗生郁李產生的原因進行了解釋。
  14. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  15. Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization, the author studied many fingerprints of the same dna, the different organic fingerprints of the same organism and fingerprints of parent and offspring. the patterns were completely different among the different strains and those of the samples from the same strain were completely identical

    採用公安部二所自行研製的jl - 02多位點探針對5個品系的近交系小鼠和2個品系近交系大鼠進行了dna指紋分析，經過對同一dna的反復製作dna指紋圖和同一個體不同組織進行的dna指紋圖製作及對親代和子代（同品系內和不同品系間雜交）間的dna指紋圖比較。
  16. Pag, hippocampus etc. ) and inhibiting ca2 + currents hi rat hippocampal and dorsal root ganglion neurons. immunohistochemical and in situ hybridization histochemical studies have demonstrated orl1 receptor, cannabinoid receptor and their ligands are densely distributed in the superficial doral hom of the spinal cord

    免疫組織化學和原位分子雜交實驗結果顯示這兩類受體及其配體在脊髓背角淺層均有大量的分佈（ antonetal . ， 1996 ; farquhar - smithetal . ， 2000 ） 。
  17. Southern hybridisations showed that the pf40 gene was present in one or few copies in the millet genome

    Southern雜交分析pf40基因的拷貝數，表明pf40基因以單拷貝或低拷貝形式存在穀子基因組中。
  18. 20 crossbred beef cattle were divided into 2 groups in order to study the effects of chinese herb medicine additive on their performance and blood biochemical parameters

    摘要選用20頭雜交架子牛，隨機分2組，研究日糧中添加中藥飼料添加劑對肉牛生產性能和血液指標的影響。
  19. The result of the northern hybridization showed that it was expressed only in floral buds

    該基因無內含子存在， northem雜交分析表明該基因在花蕾中特異表達。
  20. By yeast two - hybrid assay, aes was found to interact with gp130 intracellular region through its conserved q domain. results from the yeast two - hybrid assay, gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain, and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction. to investigate the consequence of this interaction, tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector

    在通過酵母雙雜交分析確定aes通過q結構域與sp130分子胞漿區結合的基礎上，為確定tle1分子是否也能通過保守的q結構域與gp130分子胞漿區結合，我們通過pcr擴增編碼gp130胞漿區與tleq分子不同結構域的cdna ，構建了含有這些不同結構域的酵母雙雜交載體，通過酵母雙雜交分析證實： tle1分子通過其氨基端的q結構域與gp130分子胞漿區近膜段結合。
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