雞胚卵黃囊 的英文怎麼說
中文拼音 [jīpēiluǎnhuángnáng]
雞胚卵黃囊
英文
chick embryo yolk sac-
The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev
本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。
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