電離倍增作用 的英文怎麼說

中文拼音 [diànlíbèizēngzuòyòng]
電離倍增作用 英文
ionizing multiplication
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 倍 : Ⅰ名詞（跟原數相同的量） times Ⅱ形容詞（加倍） double; twice as much
  • 用 : Ⅰ動詞1 （使用） use; employ; apply 2 （多用於否定: 需要） need 3 （敬辭: 吃; 喝） eat; drink Ⅱ名...
  1. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  2. The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev

    本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。
  3. Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods

    大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。
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