青霉素酰化酶 的英文怎麼說
中文拼音 [qīngméisùhuà]
青霉素酰化酶
英文
pa ase-
E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd
以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。A new carrier for immobilization of penicillin acylase
固定化青霉素酰化酶的新載體Progress in support material for the immobilization of penicillin acylase
青霉素酰化酶固定化載體材料研究進展Studies on immobilization of penicillin acylase to chitosan as larrier
以殼聚糖為載體固定化青霉素酰化酶的研究The purified enzyme had a specific activity of 68. 6 u / mg protein. overproduction of pga was often limited by translocation and / or periplasmic processing steps, subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm
經deae - sepharosecl6b離子交換層析和butyl - sepharosecl4b疏水層析，即可得純度提高20倍、比活為68 . 6u mg的青霉素g酰化酶，兩步純化的總得率達91 。Immobilization of penicillin acylase by intensive adsorption of chitosan on celite
硅藻土強化吸附殼聚糖固定化青霉素酰化酶Immobilization of penicillin g amidase on beaded glycidyl methacrylate copolymer support
青霉素酰化酶在甲基丙烯酸縮水甘油酯共聚物上的固定化The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga, 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm, this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step
Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青黴g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高於菌株dh5 pkkfpga 。Studies of a polyacrylic acid as a carrier for the immobilizat ion of penicillin acylase
聚丙烯酸載體用於青霉素酰化酶的固定Studies on immobilization of penicillin acylase on novel complex carrier pei silica gel
青霉素酰化酶在新型復合載體上的固定化研究Emmobilzation studies of penicillin acylase on the porous bead with oxirane groups
以環氧乙烷為活性基的多孔顆粒狀固定化青霉素酰化酶的制備A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein. the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis. it was demonstrated that constructed phagemid can still express penicillin g acylase
將包含信號肽和琥珀終止密碼子uag （ amber ）的完整巨大芽孢桿菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通過引入的11肽連接青霉素g酰化酶的c末端與噬菌體外殼蛋白gp3的n末端。The display of penicillin g acylase from bacillus megaterium not only offers the possibility of applying this technology for the selection of penicillin acylases with new side - chain specificities, but also facilitates our screen of mutant library constructed by using dna shuffling technique
這是首次在噬菌體表面展示出有活力的巨大芽孢桿菌青霉素g酰化酶，為利用噬菌體展示技術進行青霉素g酰化酶突變庫的篩選奠定了基礎。分享友人