顯微克隆 的英文怎麼說
中文拼音 [xiǎnwéikèlōng]
顯微克隆
英文
microclone-
This simple two - step chemical enucleation procedure yields fully enucleated mouse oocytes in 85. 6 % of cumulus - oocytes complexes and 56 % of natural oocytes, significantly different ( p < 0. 05 ). since the enucleation rate of cumulus - oodles complexes is equivalent to that by micromanipulation, induced enucleation will greatly improve the whole efficiency in mouse somatic nulear transfer
該方法完全丟棄了傳統核移植的顯微操作及其繁瑣程序，是純粹的手工克隆（ hmc ） ，它的成功將會大大簡化核移植程序，提高核移植效率，加快其產業化的步伐。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。Thin sections of host leaf cells infected by bbwv - 2 isolate b935, which were gold - labeled by antibodies of bbwv - 2 coat protein ( cp ) and vp37, respectively, were prepared to elucidate the locations of vp37 in cell and possible function of vp37 and cp in cell to cell movement. observation in electron microscope showed that virus particles were presented not only in cytoplasma but also in chloroplast, while vp37 was existed only in cytoplasma and associated with tubular structure through the cell wall
為研究vp37在寄主細胞中的作用機制及其在細胞中的分佈，通過膠體金間接標記6his - vp37兔抗血清，同時還標記了病毒的外殼蛋白單克隆抗體，對bbwv - 2分離物b935感染的病葉超薄切片的電子顯微鏡觀察發現：病毒粒子除了聚集在胞質中，還存在於寄主的葉綠體內； vp37蛋白能在細胞壁上形成管狀結構，在胞質中亦有分佈。This thesis studied on bacillus thuringiensis strain bt886 which was separated and selected by researchers of our laboratory. according to the observation of crystal shape and the bioassay of motschulsky and fairmaire, bacillus thuringiensis strain bt886 was identified as cry3 type, and the conclusion was assured by the further study on molecular level. the 1956 base pairs full lengrh homological cry3aa gene which was toxic to motschulsky was cloned and sequenced
以該菌株為材料，克隆出了對光肩星天牛（ anoplophralabripennis （ motsch . ） ）具有毒殺作用的cry3aa同源基因，並且對該基因進行了人工改造、人工合成、大腸桿菌表達、生物活性測定、雙元表達載體的構建以及對楊樹的轉化等一系列研究，主要結果如下： （ 1 ）顯微觀察該菌株所形成的伴孢晶體為方形。According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。Naa can not only defend the invasion of various microorganism, but maintain the stability of internal envioment, lead to self tolerance, regulate immunity and prevent tumor progression
從不同角度對naa的研究顯示： naa的產生可能與自身反應性b細胞克隆陽性選擇過程有關； naa對外可以抵禦外來微生物的入侵；對內可以維持機體內環境的穩定，引起自身耐受，調節免疫和抗腫瘤。Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence
以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。The two mediums can meet primary culture and passage culture of the black bear fibroblast cells. the method of single cell cloning by micro - manipulating purifies fibroblast cells. as a result, steady fibroblast cells can be obtained and the rates of the cloning formation is 93. 75 %
通過單細胞集落顯微法純化細胞，可克服單細胞克隆中因細胞密度太低，難以形成克隆的缺點，而且操作簡單，效率高，速度快，其克隆率可達93 . 75 。Objective the origin and the nature of the hodgkin and reed - sternberg ( h / rs ) cell of hodgkin ' s lymphoma ( hl ) has been attracting a lot of medical researchers engaged in studying it, for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells. great improvement has been made since a new method of isolation of single h / rs cells from a frozen section had been set up by kuppers in 1994, and many studies have approved of the b - cell derivation of h / rs cell. lt has been reported that h / r - s cells might partly be originated from b - cell in our research before, but at the same time, we also found that only 18. 8 % of h / rs cells express cd20, 31. 3 % of immunoglobulin heavy chain ( igh ) rearrangement have been revealed
目的霍奇金淋巴瘤（ hodgkin ' slymphoma ， hl ）由於它的惡性腫瘤細胞? h rs細胞一般只佔腫瘤組織的極少部分（不到1 ） ，且散在分佈在背景細胞間，因此對于h rs細胞的來源和性質研究一直是人們探索的目標。 1994年德國科隆大學k ppers等發展了一種從冰凍組織切片上用顯微操作儀挑取單個h rs細胞的顯微切割方法進行h rs細胞基因分析后，人們對h rs細胞來源的研究有了突破性的進展，多數支持b細胞來源。我們的前期研究也支持部分h rs細胞為b細胞來源，但我們發現只有18 . 8的h rs細胞表達cd20 ， 31 . 3的hl檢測到igh基因重排克隆性條帶。This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。The main contents and results were as follows : 1. the establishment of sd rat fibroblast for the donor of nuclear transfer the method of single cell cloning by micro - manipulating was improved to purify fibroblast. as a result, steady fibroblast can be obtained and the rate of the clone formation is 92. 71 %
主要內容和結果如下： （一） sd大鼠成纖維細胞系的建立將單細胞顯微法改進為單細胞集落克隆純化，能快速、有效建立細胞系，並可獲得92 . 71克隆率。分享友人