顯微切片機 的英文怎麼說
中文拼音 [xiǎnwéiqiēpiānjī]
顯微切片機
英文
microtome-
Equipment and instruments : electronic analytic balance, uv and vis spectrophotometer, 97rt fluorescence spectrophotometer, gas chromatograph spectrometer, high speed centrifugal machines, leica rm 2015 microtome, fluorescence microscopes, pcr amplifier, and so on
:電子分析天平、紫外可見光分光光度計、 97rt熒光分光光度計、氣相色譜儀(附4種檢測器) 、高速離心機、病理切片機、熒光顯微鏡、 pcr擴增儀等。Material and methods normal rats of male sd were divided into young, adult, and aging groups. preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0. 5ml / 100g body weight ). perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart, the whole procedure was lasted for about somin. the entire brain was dissected out and dipped in the fixative solution for 12h at 4. brain pieces targeted were choosen and then passed the graded alcohols for dehydration, dipping into paraffin for embeding, and reshaping the pieces
2 )磷酸緩沖液100400m , 30分鐘灌注完畢,取出整腦,在上述固定劑oc )內后固定12小時。切取觀察部位腦塊,然後,進行梯度酒精脫水,浸蠟,包埋,修塊,石蠟連續切片(德國leica石蠟切片機人切片厚度still , zlllll ,蛋自甘油載片撈片, 60c烤箱過夜,二甲苯脫蠟,梯度酒精置換,浸水, h六染色,梯度酒精脫水,二甲苯透明,中性樹脂封片。室溫風干后,顯微鏡觀片, olympus萬能顯微鏡照相。The morphology of single cell and the ultrastructure of cell membrane were observed. by means of afm, the ultra - thin sections of murine es cells were investigated in order to make afm capable of gaining the information of the inner structure of cells. in addition, the morphological changes and damaging effect of trichosanthin ( tcs ) on red blood cell ( rbc ) membrane were observed by afm
對原子力顯微鏡( atomicforcemicroscope , afm )的成像技術進行了多方面探索;用afm研究膠原蛋白分子在雲母表面的吸附和自組裝行為;對小鼠胚胎幹細胞和人血紅細胞進行afm成像,觀測單個細胞的形態以及細胞膜的微觀結構;利用afm得到了小鼠胚胎幹細胞超薄切片的高解析度圖像,探索用afm研究細胞內部結構,拓展其應用領域;天花粉蛋白( tcs )和紅細胞的相互作用,利用afm觀察到天花粉蛋白( tcs )和紅細胞相互作用前後紅細胞膜超微結構的變化,據此討論了二者的作用機理。Thin sections of host leaf cells infected by bbwv - 2 isolate b935, which were gold - labeled by antibodies of bbwv - 2 coat protein ( cp ) and vp37, respectively, were prepared to elucidate the locations of vp37 in cell and possible function of vp37 and cp in cell to cell movement. observation in electron microscope showed that virus particles were presented not only in cytoplasma but also in chloroplast, while vp37 was existed only in cytoplasma and associated with tubular structure through the cell wall
為研究vp37在寄主細胞中的作用機制及其在細胞中的分佈,通過膠體金間接標記6his - vp37兔抗血清,同時還標記了病毒的外殼蛋白單克隆抗體,對bbwv - 2分離物b935感染的病葉超薄切片的電子顯微鏡觀察發現:病毒粒子除了聚集在胞質中,還存在於寄主的葉綠體內; vp37蛋白能在細胞壁上形成管狀結構,在胞質中亦有分佈。In view of the above mentioned problem, the author adopts information technology such as image processing and pattern recognition to research into the method of automatic analysis and classification. in accordance with the difficulty in medical image analysis ( for example, the background of microimage of section is complicated and is difficult to be segmented. ), the paper puts forward two kinds of segmentation methods based on standardized colorful space and rgb and hsv colorful model
本文針對上述問題,用計算機圖像處理及模式識別等信息技術對顯微細胞圖像的自動分析和分類的方法進行了研究,並針對醫學圖像分析中的難點(例如,顯微切片圖像背景復雜,分割困難) ,提出了基於歸一化彩色空間和rgb , hsv彩色模型的兩類分割方法:利用模式識別技術中關于特徵向量空間聚類的方法實施真彩色分割。In the conization specimens that showed a positive margin, residual lesions were found in 84 out of 150 ( 56 % ) and 53 out of 80 ( 66 % ) in subsequent hysterectomy in the leep and ckc groups respectively
至於在錐狀切片檢體邊緣有病變組織以及子宮殘餘的病變組織機率則和子宮頸上皮病變的程度或是否已經出現顯微侵犯無關。Three - dimensional reconstruction of microscopic images ( 3drmi ) of internal structure of biological specimens is one of the most important research fields in biological engineering. it has achieved great successes in the world over last decade. however, in our country, the research of this technology is on the primary state
生物體內部結構三維顯微成像技術( three - dimensionalreconstructionofmicroscopicimagesofbiologicalinternalstructure ,簡稱3drmi )的基本思想是將生物體進行序列化切片,得到二維序列圖像,然後利用計算機對這些二維序列圖像進行三維重構和顯示,使生物體內部結構三維圖像以及各斷層面圖像得到真實的再現。Leica microsystems nussloch is a company operating within many ranges and offers paraffin embedding device, single - deck machines as well as microtomes
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