顯微注射法 的英文怎麼說

中文拼音 [xiǎnwéizhùshèfǎ]
顯微注射法 英文
microinjection
  • 顯 : Ⅰ形容詞1 （明顯） apparent; obvious; noticeable; evident 2 （有名聲有權勢的） illustrious and inf...
  • 注 : Ⅰ動詞1 （灌入） pour; irrigate 2 （集中） concentrate on; fix on; focus on 3 （用文字來解釋字句）...
  • 射 : Ⅰ動詞1 （用推力或彈力送出） shoot; fire 2 （液體受到壓力迅速擠出） discharge in a jet 3 （放出） ...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 顯微 : microadiography
  • 注射 : injection; inject; get a shot
  1. Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %

    採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵，實驗結果如下： （ 1 ）顯微注射后，根據胚胎發育分期，胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光，原腸期發熒光的胚胎比例為25 ，後期發育熒光率逐漸下降，肌肉效應期后又相對穩定。
  2. On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

    本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。
  3. It is applicable to coinject two or three genes whose expression products have no antagonistic action. 4. coinjection is a simple gene transfer method which saves time and money

    非桔抗基因（轉移基因的表達產物互不抑制）的共轉移（顯微注射法）是一種既經濟、又有效的方法。
  4. Methods : microsurgical testicular sperm extraction was conducted on to a patient suffering with noa to obtain sperms and intracytoplasmic sperm inject ( icsi ) was performed to assist pregnancy

    方法：採用睪丸切開顯微取精術獲取精子，結合卵漿內單精子顯微注射技術，輔助1例非阻塞性無精子癥不育患者人工受精。
  5. On the basis of the characteristics of the cell micro - images, three image processing methods are used separately in cell images " quick recognition and feature ' s snapping to get the fast detection of the injection volume by vision

    本文根據微注射顯微圖像的特點，利用三種圖像處理法，分別對細胞圖像進行目標快速識別及特徵提取，以實現微注射量的視覺快速檢測。
  6. The recombination gene micyoinjected is 5nl dna ( about 106 - 107 ) into everyone oocytes or cytoplasm of grass carp. there were 32197 oocytes of 12 series via micyoinjection and achived 12945 fries in 2000 year, by hatching and raising. genomic dna was extracted from grass carp who were transformated in 1120 grass carp presented postive reaction by blood

    實驗通過將hu - - ifn基因重組分子採用顯微注射法將這種重組基因轉化草魚1 - 2細胞期的受精卵，即以narashige顯微注射儀，將5nldna （約10 ~ 6 10 ~ 7分子）直接注射到選擇好的受精卵核的動物極上。
  7. The objective of this study was whether the reconstituted embryo with the second polar body ( pb2 ) can develop. firstly, female mouse was superovulated with pmsg + hcg and keep in the same cage with male mouse, the pronuclear embryo was obtained at the different time after hcg injection with different motheds to investigate the existing time of pronuclear embryo. then pronuclear embryo was cultured with three different mediums to select the best to culture in vitro

    母鼠在注射hcg后與公鼠同籠交配，並在注射hcg后不同時間（從18h到26h ） 、不同方法（體內法和體外法）獲取胚胎，研究獲取原核胚的最佳時間；在此基礎上獲得具有第二極體原核胚，然後對原核胚進行顯微操作，去除雌原核並注入第二極體，探討第二極體內遺傳物質支持胚胎發育的潛力；同時利用dna特異性染料h33342對原核胚進行染色，顯示並比較原核和第二極體內的dna遺傳物質。
  8. Related problems of method of microinjection into rabbit lumbar intervertebral disc and its anatomic feature

    兔腰椎間盤內顯微注射方法的相關問題及其解剖學特點
  9. Gene transfer is a new and widely used technology in bioscience field. at present over 80 percent of transgenic animal models are constructed by means of microinjection

    轉基因是近年來在生命科學領域廣泛應用的新興技術，目前80 %以上的轉基因動物模型是利用顯微注射法構建完成的。
  10. They were coinjected into the male prenuclei of fertilized eggs with hsa. dna together. after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings, the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection

    用顯微注射法分批把這三種不同的dna片段導人小鼠受精卵雄原核，並移植入假孕受體鼠，產生的65隻n代小鼠中，經pcr和southern雜交檢測，表明轉入的基因在12隻小鼠體內有整合段。
  11. They cannot naturally produce essential fatty acids - beneficial nutrients found mainly in plant and fish oil, so they must rely on a dietary supply. here we want to establish transgenic mice carry a tissue - specific expression gossypium hirsutum - 6 fatty acid desaturase ( fad2 ) gene which can add a double bond into an monosaturated fatty - acid hydrocarbon chain and convert oleic acid to linoleic

    本試驗試圖構建一個肌肉組織特性的- 6脂肪酸去飽和酶（脫氫酶）真核表達載體，通過原核顯微注射法把棉花的- 6脂肪酸去飽和酶導入到小鼠體內，以小鼠為動物模型，建立動物體內的必需脂肪酸合成代謝通路。
  12. Aqueous fluid volume and [ c1 ~ j were assayed in samples withdrawn by micropipettes. intraocular pressure ( top ), pressure - dependent outflow, and anterior chamber compliance were determined from pressure measurements in response to pulsed and continuous fluid infusions into the anterior chamber using micropipettes. result : in wildtype mice ( gdi genetic background, age 4 - 6 weeks ), iop was 16. 0 ? 0. 4 mmhg, aqueous fluid volume was 7. 2 ? 0. 3 ul, aqueous fluid production was 3. 6 ? 0. 2 ul / hr, aqueous fluid outflow was 0. 36 ? 0. 06 ul / hr / mmhg, and anterior chamber compliance was 0. 036 ? 0. 006 ul / mmhg ( mean ? se, 8 - 10 eyes )

    實驗方法包括：將熒光物質用電離子滲透的方法穿透角膜導入活體小鼠的前房中，然後應用共聚焦顯微鏡根據熒光強度變化測量房水生成率；通過顯微注射針吸取房水檢測房水容積和氯離子濃度；顯微玻璃管刺入前房測量眼內壓，並將生理鹽水分別以連續和脈沖兩種方式注入前房，測量房水間隙的順應性和房水排出與眼內壓的相關性。
  13. In order to enrich the content of the anatomy and histology about andrias davidianus, obtain new data about systematic position and blood circulation physiology and also provide reference for comparative anatomy and vertebrate evolution reseach, we studied the anatomy and histology about andrias davidianus by means of normal inject method, normal paraffin section method, microcopy system and digital camera

    本研究主要採用常規血管注射法，常規石蠟切片法，以及光學顯微照相系統和熒光顯微數碼照相系統對中國大鯢（ andriasdavidianus ）的循環系統進行了比較詳細的解剖學觀察研究，並對其心臟及血管進行了組織學研究。目的在於豐富中國大鯢解剖學和組織學的資料，為中國大鯢分類地位的探討和血液循環生理研究提供解剖學依據，同時也為比較解剖學和脊椎動物的進化提供解剖學依據。
  14. We also investigated the effect of c on the samples formed by ion implantation of mn and c. we studied the samples " crystal structure and surface appearance by x - ray diffraction and afm, experimental results revealed that with increasing the annealing temperature, the crystal lattice reformed and defect in the surface reduced gradually

    還進行了mn ~ + 、 c雙離子注入，研究了c對樣品性質的影響。利用x -射線衍射法和原子力顯微鏡對樣品的晶體結構和表面形貌進行了研究。發現隨著退火溫度的升高，樣品的晶格質量得以恢復；注入表面形成的晶格缺陷逐漸減少。
  15. The approach of gene coinjection was firstly used by clark ' s research team in britain, aiming to improve the expression level of foreign gene by its cooperating action. thereafter, it has been increasingly using to find out the impact of gene and its elements on expression. by now, it has been successfully used to produce transgenic mice which contain two or three genes in vivo

    外源基因的轉移方法?顯微共注射法，首先由英國的clark為首的研究小組提出，旨在研究外源基因協同作用以提高其表達水平，此後，共注射法用於研究基因和基因構件對表達的影響，並日益受到重視，目前，已成功用於建立轉雙基因小鼠的研究。
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