Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product
用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ,根據genebank中已知的黑
麴黴植酸酶基因序列設計出一對特異性引物(上游引物: 5 ataggcatcatgggcgtctc3下游引物: 5 cagctaagcaaaacactccgc3 ) ,採用pyrobest ~ ( tm ) dnapolymerase (高保真dna聚合酶) ,通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物,將其與pmd18 - tvector連接后,轉化e . colidh5菌株的感受態細胞,經質粒抽提、酶切鑒定,確認該
目的產物已得到成功克隆。
In 52 strains filamentous fungi and 82 strains macrofungi, the percentages of antitumor strains are as follows : 15. 4 % filamentous fungi, 21. 9 % macrofungi, most of which belong to aspergillus sp
52株絲狀真菌和82種大型真菌的生物活性測定結果顯示,絲狀真菌中的15 4和大型真菌的21 9具有抗腫瘤活性,主要分佈於麴黴屬中和傘菌目中。