黑麴黴酶 的英文怎麼說

Studies on the xylanase producer aspergillus niger

黑麴黴產木聚糖酶的研究

Purification and some properties of tannase from aspergillus niger

黑麴黴單寧酶的純化及部分性質研究

Study on the culture conditions of aspergillus to produce cellulase

黑麴黴產纖維素酶液體發酵條件的研究

Purification and properties of the cellulase from aspergillus niger

飼用黑麴黴纖維素酶的純化及酶學特性的研究

Studies on screening of strains of producing phytase and the conditions of producing phytase the strains of producing phytase could be identified by the hydrolysis bound in differential medium. aspergillus niger an010001 secreting phytase was isolated by screening and second screening. the conditions of producing phytase was studied

植酸酶菌株的篩選及產酶條件的研究本項研究利用植酸酶的菌株能在篩選培養基上形成水解透明圈的特點而進行鑒定，通過初篩和復篩，得到一株產植酸酶較高的黑麴黴（ aspergillusniger ） an00101菌株。

The principle and method for enzymatic synthesis of gallic acid, isolation and selection of the aspergillus niger strains, characteristics of this biotechnology, products quality of gallic acid and the uses in domestic food and pharmaceutical industries are briefly introduced

摘要概述了黑麴黴單寧酶酶法轉化五倍子單寧酸生產沒食子酸的原理和方法、酶源菌種的分離和選育、工藝技術的特點、產品質量規格及在國內食品、醫藥行業相關部門的應用等。

At present, most of lactases used in industry production come from kluyveromyces, aspergillus niger and aspergillus oryzae

目前，工業生產中使用的乳糖酶主要來源於乳酸克魯維斯酵母菌、黑麴黴和米麴黴。

Breeding of asperillus niger with high production of acid proteinase

高產酸性蛋白酶黑麴黴菌種選育

Two novel cellulases were identified and isolated to homogeneity from a commercial aspergillus niger cellulase preparation. one was an endo - l, 4 - - glucanase ( ec

本文是關于黑麴黴（ aspergillusniger ）中兩種纖維素酶的分離純化與表徵及其動力學作用機制的研究。

Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。

Purification and properties of an endo - - glucanase from a commercial preparation of aspergillus niger

黑麴黴產纖維素酶系中內切酶的純化和性質

Characteristic of respective components from cellulase system induced by aspergillus niger and condition of enzymolysis

黑麴黴產纖維素酶系各組分特性及酶解條件

Study on property of extracellular pectinase from aspergulus niger

黑麴黴胞外果膠酶特性的研究

Primary study on pectinase processing condition by liquid - state fermentation with aspergillus niger

黑麴黴液態發酵生產果膠酶的工藝條件初探

Study on the technologies of liquid - state fermentation for producing pectinase with aspergillus niger

黑麴黴液態發酵生產果膠酶的工藝條件初探

Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。

3. 2. 1. 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25, and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography. the other was a p - glucosidase ( ec. 3. 2. 1. 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography. the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433. 38 hj / mg

-葡萄糖苷酶對水楊素的比活力為597 . 12iu mg ，並對其專一，不能水解棉花和羧甲基纖維素鈉；分子量為117 . 5kda ，加dtt後分子量不變；該組分最適ph和溫度分別為4 . 5和70 ，在ph5 . 0 、 50下對水楊素鈉的米氏常數km為3 . 73mg ml ，最大反應速度vm為0 . 088mg葡萄糖（ ml ? min ） ；與文獻中從黑麴黴中分離的-葡萄糖苷酶比較后發現，該組分是一個新的-葡萄糖苷酶。

The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。

High active phytase producing fungs - aspergillus niger were selected by mutiple uv mutation, the definitions of phytase activity were analysised and the measure wavelength of the enzyme was modified, the factors that influence the preparation of protoplast were investigated. base this, use protoplast - uv mutation and protoplast fusion to filter phytase produce asp. niger

本文以多輪紫外誘變為主線技術篩選植酸酶的黑麴黴高產菌，分析比較植酸酶酶活定義和植酸酶酶活測定方法並修正其測量波長，考查黑麴黴原生質體制備的影響因素，並在此基礎上，用原生質體紫外復合誘變和原生質體融合技術篩選植酸酶的黑麴黴產生菌。

Purification and characterization of phytase from aspergullus niger a 3214 and a. niger n

2株黑麴黴植酸酶的分離純化及其酶學性質研究