binding domain 中文意思是什麼
binding domain
解釋
結合區域-
Recently, ldl apheresis has been applied in clinic and achieves a satisactory effect. in this dissertation, the tripeptide, serine - aspartic - glutamic acid ( sde ), which existes in the cooh - terminal end of the seven repeats in the ligand binding domain of the ldl receptor and plays an important role in identifying ldl, was synthetized and immobilized onto the polyacrylamide ( paam ) beads as a bionic adsorbent for selective removal of ldl from plasma
本論文以絲氨酰-天冬氨酰-谷氨酸（ sde ）負電性三肽（此三肽廣泛存在於ldl受體配體結合域7個重復序列的羧基末端，對ldl受體特異性識別ldl起著重要作用）作為配體固定到聚丙烯酰胺微球載體上製成仿生性ldl親和吸附劑，考察其對人血漿中ldl及hdl的吸附功效。 -
Homologues of era have been identified in prokaryotes and eukaryotes. there are two domains in era : the n - terminal gtp - binding domain and c - terminal rna binding kh domain. era plays an important role in cell cycle progression at a specific point in the cycle, after chromosome partitioning but before cytokinesis
Era是在原核和真核生物以及植物中普遍存在的g蛋白，是一個結構上既具有gtp結合結構域又有rna結合結構域、不同於ras的獨特的新的g蛋白亞家族。 -
Sox genes encode a group of proteins that carry a dna - binding hmg domain
Sox基因含有一個能與dna特異結合的高度保守hmg - box基序。 -
Main methods and results are as followed : 1 epitope analysis of agonist - binding region of nrla physicochemical properties and antigenicity of two agonist - binding regions of nrla were analyzed through bioinformatics : domain p1 containing 151 amino acid residues preceding the first transmembrane domain of the human nrla, domain p2 with 144 residues following the third transmembrane domain. four parameters including hopp - woods and kyte hydrophilicityjanin accessibility, karplus - schulz flexibility, and welling antigenicity were used to determine the antigenic sites, and prosite programme and chou - fasman method were employed to analyze their related sequence motif and the secondary structures
用goldkey軟體分別選取公認的hopp等與kyte等親水性參數、 jain表面可及性參數、 karplus - schulz主鏈柔韌性參數及welling抗原性參數對p1 、 p2兩個多肽片段進行參數分析。並採用通用的prosite程序與chou - fasman方法比較分析p1 、 p2多肽片段的氨基酸位點與二級結構特徵。綜合判定兩個多肽片段的抗原性及其位點，結果認為p2抗原性強于p1 。 -
To neutralize the function of cam, the cam binding domain peptide from bovine calcineurin a subunit was chosen because the pre - experiment demonstrated that bovine calcineurin could bind to calmodulin strongely in an acidic and high calcium concentration condition which is similar to the cell wall environment. in order to increase the binding ability of the chimeric secreted calmodulin binding peptide, the plasmid containing two copies of calmodulin binding domain were constructed
它共有26個氨基酸，具有信號肽的典型特徵。為了削弱質外體cam的功能，選擇了牛腦鈣調神經磷酸酶can （ calcineurin ）的a亞基的cam結合域。預實驗結果表明，在近似於胞外環境的酸性高鈣條件下can與cam有強結合。 -
To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。 -
These three proteins contain two common clif domains, ubox and arm repeats. ubox is putative domain of dimeration of arc1 protein or the binding domain of next element
這種ubox結構均位於氨基酸序列的中間區域，這個區域有可能是蛋白質發生二聚化的區域或下一級組分的結合的位點。 -
Predicted tertiary structure showed that thl2 protein possessed a domain, and the srk - binding site cppc is located in tertiary structure surface, which provided the spacial foundation for thl2 binding srk kinase
預測的thl2蛋白質高級結構中有一個結構，並且其與srk結合的活性位點cppc位於蛋白質的表面，這為thl2蛋白與srk激酶結合提供了空間上的基礎。 -
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。 -
Screening and constructional analysis of extracellular at the ligand binding domain of the vascular endothelial growth factor receptor flt
胞外配體結合域的篩選及其結構分析 -
Ie acts as a general gene transcription activator. it consists of dna - binding domain and acid amino acid transcription activate domain. what kinds of promoter can be stimulated by ie 180
另外ie180與hsv的立即早期蛋白icp4有較大的同源性，研究ie180轉錄調控作用有利於皰疹病毒轉錄調控模型的建立。 -
By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq
然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。 -
Following researches verified that the cam - binding domain exists in the c - terminal of ecbp21
通過缺失表達分析實驗證實cambd結構域位於ecbp21的羧基端。 -
The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。 -
The predicated amino acid sequences contain two conserved functional domains : a dna - binding domain ( hmg - box ) and a transactivation domain. the conserved motif of group b homology lies immediate adjacent to c - proximal region of the hmg - box. similar structure and identity of sox2 gene among mammals, birds, amphibians and reptiles suggest that sox2 gene have evolutionary conserved roles
進一步分析表明，已巴灘2編碼的238個氨基酸多膚鏈含有兩個保守的功能區域:位於n端的hmg盒區（ 1一78aa ）和位於c端的轉錄激活區（ 131一236aa ） ，以及一個保守的特徵性b亞族同源區域（ 79一90aa ）與hmg盒區的c端相連。 -
The vfcpkl has an obvious kinase catalytic domain for ca2 + / calmodulin dependent protein kinases and serine / threonine protein kinases, and a calcium - binding domain like calmodulin
Vfcpk1具有明顯的ca ~ （ 2 + ）鈣調素依賴蛋白激酶及絲氨酸蘇氨酸蛋白激酶的激酶區以及類似於鈣調素的鈣結合區。 -
It is show the mutant consists of a nuclei binding site and dna - binding domain is enough to negative regulation. a report plasmid pcat that we constructed has a cat gene expressed by a cmv early promoter
（ 465 1079氨基酸段）對sv40啟動子明顯的抑制活性，因為它只包含dna結合域和核結合位點，說明發揮轉錄抑制功能的關鍵是ie180的dna結合域。 -
We also predicted the cam binding domain of ked like protein and showed that the cambd lies in its c terminus
對這一蛋白的cam結合域進行了預測，表明ked樣蛋白的cam結合域在其c端。 -
2. a bhlh - type gene osbhlhl was obtained from the rice cdna library by using the rapid cloning pcr. the predicted osbhlhl protein had a nucleus location signal, a putative dna binding domain contained a bhlh - bzip domain
2利用pcr篩庫法，從水稻cdna文庫中克隆到一個bhlh類型的基因，命名為osbhlh1 ，它的推測蛋白結構中含有一個細胞核定位信號和一個可能的與dna結合bhlh - zip結構域。 -
The fusion gene ofgfp : mtn ( mtn is binding domain of microfilament binding protein talin, which can show the microfilament in living cell ) was transferred to arabidopsis thaliana. stable expression of gfp - mtn can visualize the actin cytoskeleton in different types in living cells without affecting cell morphology and function
將gfp : mtn （ mtn是微絲結合蛋白talin的微絲結合域，可以顯示活體細胞中微絲的結構）導入擬南芥后，發現表達的融合蛋白能夠標記微絲骨架，同時又不影響植物細胞的正常形態和功能。
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