clone cell 中文意思是什麼

clone cell 解釋
純種細胞,克隆細胞
  • clone : n. 1. 【生物學】純種細胞，無性系。2. 復製品；（幾乎）一模一樣的人。3. （不動腦筋）機械行事的人；機器人。vt. 把…培養為純種細胞；無性繁殖；〈比喻〉復制。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. T cell will be apopotosis, clone depletion and / or anergy, in turn inducing an antigen - specific tolerance. ctla4 ( cytotoxic t lymphocyte associated antigen 4 ), a prominent negative costimulator, can competitively bind with b7, and block b7 - cd28 pathway and t cell activation by producing inhibitory singals

    Ctla4 （ cytotoxictlymphocyteassociatedantigen4 ）作為一種主要的共刺激負調節劑，可競爭性結合b7分子，阻斷b7 - cd28途徑、並通過產生抑制性信號使t細胞活化障礙。
  2. One of australia ' s pre - eminent stem cell researchers has challenged the federal health minister tony abbott saying only a scientist " on the lunatic fringe " would be tempted to fully clone a human being

    一位澳大利亞卓越的幹細胞研究者，曾對聯邦衛生部部長東尼.阿伯特提出質疑說，如果一個科學家處于「快要瘋了的邊緣」 ，那麼他才會備受完整地克隆一個人的誘惑。
  3. There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i

    G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。
  4. An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined

    將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。
  5. Full - length clone of protein ht036 gene interacting with endostatin and its molecular mechanism in promoting the apoptosis of vascular endothelial cell

    036基因的全長克隆及其促血管上皮細胞凋亡的分子機制
  6. Then the linked products were transformed into the high competent cell of e. coli dh5a. based on - complementation of the detective - galactosidase, positive recombinant clone were screened from x - gal plate

    從引物和基因序列的比對分析結果看， zhyf006序列與上下游引物的配對比例分別為s4
  7. Plasma cell clone

    漿細胞純系
  8. Mutation can also occur in cells other than germ cells - somatic mutation ? but this is likely to be apparent only when the genetically altered cell proliferates abnormally to form a large family or clone of similar cells, e. g. when a tumour forms

    突變也可發生於生殖細胞以外的細胞（體細胞突變） ，但這很可能僅表現于遺傳性改變的細胞異常增生而形成一大系或克隆的相似的細胞時，例如當一個腫瘤形成時。
  9. Moreover, if the embryo from which such cells were derived happened to be a clone of an existing person, they would not be recognised as foreign by the immune system of their progenitor and might thus be used as treatments for diseases that require the replacement of a particular, lost cell type

    而且，如果提供細胞的胚胎恰巧是一位活人的，那麼這些細胞不會被胚胎提供者的免疫系統認為是外來的（不會產生排異反應） ，這樣就能為一些疾病提供治療，這些疾病需要替換一種特殊的無用的細胞。
  10. The highlighted mass production of astaxanthin using h. pluvialis remains problematic since the inhibition of cell division occurs while astaxanthin produced. it has been proved that biosynthesis of astaxanthin is regulated, at least partially at level transcription. this paper is intended to clone and character the cis regulatory sequences of genes of key biosynthesis enzyme

    已有實驗結果證明，蝦青素的合成調控至少部分發生在合成酶基因的轉錄水平上，提示從分子水平上研究蝦青素合成酶的轉錄調控機制，將有可能為人工調控蝦青素的代謝提供新思路。
  11. The characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper

    分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性
  12. Abstract : the characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper

    文摘:分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性
  13. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  14. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  15. The aim of the new cell bank is to store, characterise and clone cells and distribute them as required to researchers around the world

    新的細胞庫的目的是存儲幹細胞，研究細胞的特性，並進行無性繁殖，然後按需要將它們分配給世界各地的研究者們。
  16. In our experiment, the clone we used almost all died under 25. in the same condition, the female and male clone cell that have mostly same number have close growth speed

    光照對海帶配子體克隆的生長起著至關重要的作用，光照時間和光照強度直接影響著克隆細胞的生長。
  17. " successful cloning of an increasing number of species confirms the general impression that it would be possible to clone any mammalian species, including humans, " said ian wilmut, a reproductive biologist at the university of edinburgh who produced the first cloned mammal, dolly the sheep, from an adult cell nearly a decade ago

    愛丁堡大學再生生物學家伊恩維爾莫特稱： 「成功克隆越來越多的物種證明所有哺乳動物都是可能被克隆的，包括人類本身。 」維爾莫特是第一個克隆哺乳動物的科學家， 10年前他成功利用成體細胞克隆了綿羊多莉。
  18. After the examination of pcr, one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product, a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut

    經pcr檢測后，有一個不發光細胞克隆擴增出了與對照質粒ploxifn相同的約1000bp的條帶，表明發生了替換反應；另一個不發光細胞克隆認為是在cre重組酶的作用下其內部自行發生了剪切作用，剪切了gfp基因，從而只擴增出了載體序列和一個lox位點的約500bp的條帶。
  19. The main contents and results were as follows : 1. the establishment of sd rat fibroblast for the donor of nuclear transfer the method of single cell cloning by micro - manipulating was improved to purify fibroblast. as a result, steady fibroblast can be obtained and the rate of the clone formation is 92. 71 %

    主要內容和結果如下： （一） sd大鼠成纖維細胞系的建立將單細胞顯微法改進為單細胞集落克隆純化，能快速、有效建立細胞系，並可獲得92 . 71克隆率。
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