cloned culture 中文意思是什麼
cloned culture
解釋
克隆培養物-
It is interesting that pma plus calcium ionophore a23187 can inhibit pma - induced pta1 expression, and this effect ca n ' t be reversed by calcmeurin inhibiter fk506. pta1 mabs can inhibit ctl activation and differentiation in mixed lymphocyte culture system when added at the beginning of the culture but can induce platelet activation and aggregation in the fc dependent manner. in 1997, pta1 cdna was cloned from cdna library of tpa activated jurkat cells, which belongs to immunoglobulin superfamily ( igsf ) with two v - like domains of extracelluar region of pta1
Il - 2 、 tnf - 、 pma可以使t細胞pta1表達上調， tgf -可以下調pta1的表達，而pma加上鈣離子載體a23187可以顯著抑制pma的上調作用，且這種抑制作用不被calcineurin抑制劑fk506所逆轉， 1997年burns教授從pma活化的jurkat細胞cdna文庫中克隆了pta1cdna全長，證實pta1是一個新分子，屬于免疫球蛋白超家族，胞膜外區有兩個v樣結構域。 -
2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody
選其中一株3 - h _ （ 11 ）做雜交-雜交瘤細胞染色體計數，同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數：制備腹水型和上清型抗體，用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體，即紅細胞固相吸附法測雙特異中文摘要性抗體，紅細胞凝集試驗測單特異性抗人a型紅細胞抗體，間接elisa法測抗p24抗體；用腹水型抗體做耐熱性及耐凍融實驗。 -
Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。 -
After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。 -
The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e. coli bl21 ( de3 ). the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna編碼區序列被克隆進原核表達載體pet - 30a ，並在大腸桿菌bl21 （ de3 ）中誘導表達，但過量表達的蛋白主要是以不溶性蛋白形式存在。 -
Object : the culture medium and culture conditions of psb will be optimized and gene ubia will be cloned for the construction of recombinant strain producing and foundation of the fermentative technology in large scale
目的：優化psb產coq _ （ 10 ）培養基及培養條件和克隆ubia基因，為獲取高產coq _ （ 10 ）基因工程菌及確定規模化發酵工藝奠定良好的基礎。
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