cloned dna 中文意思是什麼
cloned dna
解釋
克隆ＤＮＡ-
It was originally cloned at the dna level from the unicellular green alga chlamydomonas reinhardtii
最初，它是在一種綠藻chlamydomonasreinhardtii中發現的。 -
In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank
本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。 -
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。 -
Two primers, designed according to the conserved regions of ban gene in arabidopsis thaliana, were used to amplify the ban homologous fragments from the genomic dna of brassica napus, b. chinensisl, b. juncea, a. thaliana and other cultural plants of cruciferae. the very similar pcr fragments were obtained from all the amplifications, which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae. pcr fragments were cloned and confirmed by restriction enzyme digestion
參照擬南芥（ arabidopsisthaliana ） ban基因與cdna設計引物，對甘藍型、白菜型、芥菜型油菜，擬南芥及其它十字花科栽培品種的基因組dna進行pcr擴增，均擴增出與擬南芥ban基因擴增片段大小極其相似的dna片段，提示ban可能廣泛存在於十字花科植物中。 -
We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0
我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。 -
At first. then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr. following that, these gene fragments and plasmid vectors, pbluescript ii ks + and puc18, were cut by bamh i and kpn i. the prepared insert dna and vector dna were linked by t4 dna ligase
利用vectornti6 . 0軟體，對所克隆的序列用相鄰接點法（ neighborjoining州j ） method ）進行多序列比對，分析其同源性，並構建基因進化樹。 -
Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced
首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。 -
The association of larval and adult stages of ecologically important caddisfly ( insecta : trichoptera ) using mitochondrial dna sequences larval and adult amplification fragments were cloned and sequenced. dna sequences were aligned using clustal x software ( gene codes corporation )
線粒休dna一coi 、 u基因序列在毛翅目成幼蟲聯系中的應用研究擴增並測定了4種鱗石蛾的成、幼蟲的mtdnacoi 、 coh及trna一leu基因序歹『 j 。 -
In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector
先用t4dnapolymerase構建成模板，再通過高保真dna聚合酶（ pyrobesttmdnapolymerase ） pcr進行擴增，從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。 -
The experimental procedure that begins with a cloned segment of dna, or a protein sequence, and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function
在已知dna克隆片段或蛋白質序列上引導程序性的變異（通過直接誘變）並返回到基因組，來研究基因和蛋白質功能的實驗方法。 -
Through primary pcr. and finally the recombinant dna fragments were cloned into the phagemid pcantab 5e vector and introduced into
十五肽的接頭連在一起，並克隆到噬菌質粒pcantab 5e中，電擊轉化大腸桿菌tg1 。 -
Firstly, the hyaluronate synthase ( has ) gene ( hasa ) was cloned into the cloning vector puc19 by pcr using the total dna sample of s. equi as template
本文研究代謝工程理論，對本實驗室的一株ha生產菌（ streptococcusequi ）進行了ha合成與分解關鍵酶基因及功能的研究。 -
705bp dna fragment of mxnrampl gene and full cdna of mxlrtl gene which were related to resist iron stress were cloned by using malus xiaojinensis cheng et jiang - the first iron - efficient genotype in the genus malus in the world as material. ( 1 ) using fragment of nramp gene from wheat and fe ( ll ) - transporter gene fragment of maize ( zmlrt ~ ) as probes, we analysed these genes by blotting hybridization technique in malus xiaojinensis cheng et jiang
本實驗以中國農業大學園藝植物研究所篩選到的一個蘋果鐵高效基因型? ?小金海棠（ malusxiaojinensischengetjiang ）為試材，分別克隆了小金海棠的抗缺鐵相關基因mxnramp1基因的752bp基因組dna片段和fe （ ） -轉運蛋白基因（ mxirt1 ）的cdna全長，為深入探討小金海棠抗缺鐵的分子機理奠定了基礎。 -
The 496 bp fragment of the orf of p22 gene and a 561 bp fragment were amplified from the genomic dna of zs strains of toxoplasma gondii. both 496 bp and 561 bp fragments were successfully cloned into the plasmid pthiohisa, b, c and pbudce 4. 1 respectively. 2
從弓形蟲zs株基因組中擴增出p22編碼基因的一長496bp ，另一長561bp的片段，並成功構建含p22編碼基因的原核質粒重組體pthiohisa ， b ， c / p22 ，及真核重組表達質粒pbudce4 . 1 / p22 。 -
Cc was cloned from a cell taken from near the ovary of a tortoiseshell cat, but she is not a carbon copy of her dna donor
茜茜是由一隻花貓卵巢旁的細胞克隆而來，但是她和她的"母體"並非長得一模一樣，她們的皮毛花紋還是有所不同的。 -
Arc1, thl1 and thl2, the substrate protein genes of s receptor kinase, were cloned through a series of methods of molecular biology such as pcr, rt - pcr, dna cloning and sequencing. the resultings sequences were highly analysed by using the related biosoftwares on internet, providing new insights in the field of the molecular mechanism of self - incompatibility in plants. the major results are as followings : 1
本文通過pcr和rt - pcr等一系列分子生物學方法克隆了蕓薹屬植物中的甘藍和油菜自交不親和信號傳導過程中srk底物蛋白基因arc1 、 thl1和thl2 ，並使用各種相關生物信息學軟體對srk底物蛋白基因序列進行了分析，然後在internet網上利用在線軟體對蛋白質的結構和功能進行了預測和探討，以期為蕓薹屬植物自交不親和性的分子機理的研究提供新的內容。 -
In this article, the author amplified dna sequences encoding the mature peptides of human intact and truncated igf - 1 gene from human genomic dna by the new way of alternative pcr established and named as exons - piecing together method by this laboratory. the igf - 1 and des ( l - 3 ) igf - l gene were cloned in pgem - t vector and then subcloned in expression plasmid pgex - 3x. two recombinant constructs known as pgex - 3x - igf - l and pgex - 3x - des ( l - 3 ) igf - l were transformed to e. coli dh5 a respectively
我們利用外顯子拼接法從人的基因組dna中擴增了編碼完整型及截短型人igf - 1基因成熟肽的dna片段，並將其克隆至pgem - t載體中；經測序證明正確后，又構建了表達載體pgex - 3x - igf - 1及pgex - 3x - des （ 1 - 3 ） igf - 1 ，在大腸桿菌中進行了表達研究。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
To prove that the cloned dna fragment can express tryptopanase, a new plasmid pet28c - tnaa, in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ), a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter, which is inducible by iptg
用iptg誘導表達t7rna聚合酶，以表達質粒上的目的基因。在葡萄糖存在的條件下，用常規方法發酵和誘導（ 37 1mmiptg ） ，發現表達的蛋白質條帶的分子量與理論上計算的分子量一致。但是發酵液中檢測不到吲哚，表明雖然表達了目標蛋白，但表達的蛋白質沒有酶活性。 -
After enzyme restriction and sequencing analysis, the nucleotide data had been further analyzed by antheprot 5. 0 and clutalw softwares. the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt, it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda
應用antheprot5 . 0 、 clustalw等分子生物學軟體分析，顯示主要外膜蛋白前24個氨基酸是較強的疏水性區域，可組成信號肽，其與omp基因的同源率達96 ，氨基酸的同源率高達98 。
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