cloned sequence 中文意思是什麼

cloned sequence 解釋
克隆片段
  • cloned : 克隆陰謀
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  3. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  4. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  5. The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector. their cdna sequences were determined with positive clones or purified pcr product. conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province, their homology is 90. 6 %

    結果：其中一對引物擴增得到一600bp產物；另一對引物擴增得到三條特異性的dna條帶，大小分別為1 . 5kb 、 1 . 3kb和800bp ，將600bp和800bpdna進行克隆及測序，並推導編碼的氨基酸序列。
  6. We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0

    我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。
  7. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  8. Envelope gene gp85 of imc10200 subgroup j avian leukosis virus was cloned and expressed in the present study. the sequence encoding the gp85 domain of imc 10200 alv - j was amplified from pgem - imc2. 2 vector, which contains env gene of alv - j imc 10200 strain, and cloned into transfer vector pfast bacl

    為深入探討alv - j的亞群特性，本研究利用alv - jgp85基因兩側的序列片段為引，物從正常spf蛋雞、商品肉雞和df1細胞基因組中完整地擴增了內源性類alv - jgp85基因。
  9. Used as 1. 1094 strain of bacillus subtilis as researched material, the structural genes of biotin operon ( bio operon ) were cloned, and its sequence were engineered

    本研究以枯草桿菌asl . 1094菌株為研究材料，克隆了生物素操縱子基因，並對基因序列進行改造。
  10. Homology analysis blast analysis showed that the amino acid sequence of the asf gene cloned from cotton was significantly homologous with adp - ribosylation factor ( asf ) of mammalian, plant and yeast, etc. the amino acid sequence of the gene shows 99 % ( 179 / 180 ), 98 % ( 177 / 180 ), 96 % ( 174 / 180 ) and 92 % ( 168 / 180 ) identity to arabidopsisthalianaarfl, triticum aestivum, solatium tuberosum and bos taurus, respectively

    棉花arf基因的同源性分析應用ncbi進行dna序列的相似性分析，結果表明：棉花arf基因與其它植物、動物和酵母的arf基因具有較高的同源性。棉花arf基因編碼的氨基酸序列與擬南芥，小麥、馬鈴薯、牛的同源性分別達到99 （ 179 / 180 ） 、 98 （ 177 180 ） 。 96 （ 174 180 ）楊花腸p核符吞fi曰手谷回伽皿的夭險和92 （ 168ill ） 。
  11. Stilbene synthase genes ( l, 2 ) from vitis venefera were cloned and sequenced. the results of sequence analysis showed the total length of s2 is 1617bp, the homology between s2 and pvin ( reported ) is 99 % ; the total length of si is 1622bp, the homology between si and pvin ( reported ) is 98 %

    Dna序列分析結果表明，來自雷司令的芪合酶基因全長1617bp ，與文獻報道的該基因（ pvin ）比較，堿基同源率為99 ，來源於粉紅玫瑰的芪合酶基因全長1622bp ，與pvin的序列比較，堿基同源率為98 。
  12. The mature region cdna of melittin of apis cerana was cloned by using puct - vector developed from pucis vector. the sequence was compared with the corresponding region of melittin of apis mellifera reported in genbank, the mutant site was found in 33rd base ( a : apis mellifera ; g : apis cerana ), but the primary structure of the two proteins were identical, which suggested melittin, as an important defensive gene, was very conservative in evolution

    測序結果顯示與意蜂（ apismellifera ） melittin成熟區序列相比，中蜂（ apiscerana ） melittin成熟區序列在第33位存在差異（意蜂為a ，中蜂為g ） ，但兩者蛋白質一級結構相同，說明melittin作為蜜蜂一種重要的防禦基因在進化上保守。
  13. Further analysis showed that there are regulatory elements, including tata box and caat box, etc. pc - igs fragments were cloned and sequenced from five strains of arthrospira ( a. platensis fachb341, a. platensis fachb439, a. platensis ouqds6, a. maxima ouqdsm and a. platensis fachb791 ) and two strains of spirulina ( s. subsalsa fachb351 and spirulina fachb440 ). the cloned fragment includes 494 bp of cpcb, 252 bp of cpcaand 111 bp of pc - igs sequence

    在節旋藻和螺旋藻分子系統學研究中，克隆了節旋藻和螺旋藻的藻藍蛋白操縱子中的pc - igs序列並進行序列測定和分子系統學分析，結果如下：所克隆的長度為857bp的片段包含有494bp的cpcb基因， 111bp的pc - igs序列和252bp的cpca基因。
  14. The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods

    根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。
  15. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  16. 14 out of 25 strains of biocontrol agent streptomyces showed chitinolytic activity by biological assay. these 14 strains of biocontrol agent streptomyces were detected for chitinase gene using pcr primers derived from the conservative sequence of 17 cloned chitinase genes from streptomyces

    此方法相對于幾丁質酶的傳統生物學檢測，具有更好的準確性和靈敏性；而且與生物學檢測相比，使用分子檢測省時省力。
  17. The experimental procedure that begins with a cloned segment of dna, or a protein sequence, and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function

    在已知dna克隆片段或蛋白質序列上引導程序性的變異（通過直接誘變）並返回到基因組，來研究基因和蛋白質功能的實驗方法。
  18. And many important cis - acting regulatory elements were found in the cloned sequence region by plantcare software analysis. ref promoter was predicted to be most probably induced by light, hot, gibberellin, ethenen or wound

    經plantcare軟體分析發現序列中含有多種調控元件，經預測ref啟動子可能被光、熱、 ga 、乙烯或傷所誘導。
  19. In order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter

    根據pr - 1a基因的報道序列，設計兩對引物，以煙草基因組為模板，通過pcr擴增得到900bp （ ipl ）和603bp （ ips ）兩個目標片段，序列測定表明克隆的啟動子與報道序列具有99的同源性，轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。
  20. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
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