cloned gene 中文意思是什麼

cloned gene 解釋
克隆化基因
  • cloned : 克隆陰謀
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. The chs gene which is obvious correlated with anthocyanin accumulation was cloned as well

    對與花青素積累有明顯相關的chs基因進行了克隆。
  3. It has been established the existence of a few pathways controlling flowering of plants in genetic research of flowering of arabidopsis thaliana and antirrhinum majus. many genes related to flowering have been identified and cloned, but the regulation mechanism of gene ' s expression is still unclear

    通過模式植物擬南芥和金魚草開花機理的研究，現在已經明確植物的開花途徑有多條，並識別和分離了許多與開花有關的基因，但這些基因如何在植物生長發育過程中進行特定時空的表達及其調控一直是科學家研究的一個黑匣子。
  4. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  5. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  6. Methods and applications of inserting a stop codon into a cloned gene with pcr

    在已克隆基因末端添加終止密碼的方法與應用
  7. We report here the cloning of a homolog of this gene from dunaliella salina. this cloned gene produces as protein with photolyase activity when expressd in escherichia coli

    運用blast方法對est序列進行分析，初步預測該est序列為光裂合酶藍光受體蛋白質家族中一個成員的部分cdna 。
  8. According to the cloned gene cluster, people can use genetical technology to obtain the genetical strains which can produce more potent and non - toxical avermectins and its derivatives

    這使得人們可利用基因工程技術來構建工程菌，增加有效組分的產出和生產新型的阿維菌素。
  9. We confirmed that the cloned gene dreb1c was really transformed into arabidopsis by pcr method, in which sequences of camv 35s were used as forward primer

    提取轉基因植株的基因組dna ，進行pcr反應，證明了dreb1c確實轉入擬南芥中。
  10. No homologous sequence was found by comparing it with the sequences obtained from the net, so that the cloned gene was supposed to be an unknown new gene, designated as opra

    同源比較分析沒有發現同源序列，推測這一序列可能是一未知的新基因，命名為apra 。
  11. 2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence

    異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。
  12. Constitution ' s prohibition of slavery to explain why a patent cannot be issued on an actual human or on his or her body parts. ) a patent on an isolated and cloned gene and the protein it produces grants the owner exclusive rights to market the protein ? say, insulin or human growth hormone ? in the same way that a chemical manufacturer might purify a b vitamin and file for a patent on it

    分離出的基因、選植出的基因以及這些基因產生出的蛋白質等相關的專利，將讓持有人享有獨家銷售蛋白質產物（譬如說胰島素或人類生長激素）的權利，就像化學藥廠可能純化出一種維生素b並申請專利一樣。
  13. 3. the cloning of a - amylase gene : the methods of shotgun, pcr and rt - pcr were selected to clone a - amylase genes from bacillus subtilis hn503, xanthomonas campestris pv. campestris 8004 and aspergillus oryzae, hn504 the recombinant plasmid with cloned gene was designated as phn504, phn503 and phn8004

    本研究分別選用了鳥槍克隆法、 pcr和rt - pcr克隆法，成功地克隆到枯草芽胞桿菌（ bacillussubtilis ） hn503 、野油菜黃單胞菌（ xanthomonascampestrispvcampestris ） 8004和米麴黴（ aspergillus口盡留『 ） hn504的價澱粉酶基因。
  14. The gene recombinant strain no. 42 ca n ' t generate ampramycin, which indicated that the cloned gene is involved in apramycin biosynthesis in s. tenebrarius

    通過接合轉移的方法將質粒pzxb014導入黑暗鏈黴菌h6中，篩選基因發生重組的菌株。
  15. The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316

    Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。
  16. Make all these together, it proved that the cloned gene represented the major outer membrane protein gene of ahl316, and the expressed gene products shared identical antigenicity with the natural main outer membrane protein. the studies on preparation and application of momp - iscoms of ah l316 provided a new approach to fish vaccinology. the successfully cloning and expressing the major outer membrane protein gene of ah l316 made it possible to describe this gene ' s function under a single factor level, and also provided technical support for developing an advanced gene engineering vaccine and subunit vaccine against aeromonas hydrophila

    鰻源嗜水氣單胞菌l316主要外膜蛋白免疫刺激復合物的制備與應用研究，對研製魚類疫苗學問題進行了新的初步探索；成功地克隆和表達嗜水氣單胞菌l316主要外膜蛋白基因為在單因子水平上研究嗜水氣單胞菌外膜蛋白的作用和免疫功能以及制備嗜水氣單胞菌基因工程疫苗和亞單位疫苗奠定技術基礎。
  17. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  18. So the cloned gene ( orf 192 ) may be a new member of human beta peptide antibiotics family, being termed as human peptide antibiotics - & ( hpab )

    結果表明克隆到的基因極可能是人p一型膚抗生素家族中的一個新成員，將之命名為hpab一p （ humanpeptideantibioties一p ） 。
  19. The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed. the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold. in order to further analyse the epitope features of the protein relevant to the specific ige antibody, the phage display library of random peptides was screened by pooled sera with the specific ige antibody

    藉助現行生物信息學分析手段，通過網際網路進入genbank數據庫，對sj43b與已知序列進行同源性比對，應用dnatoois軟體對其編碼蛋白序列的氨基酸紐成、親水性、抗原性和可及性進行分析，並在blastp程序下在genbank蛋白數據庫中對sj43b編碼3人卞醫科大學博士學杠論文蛋白進行同源性檢索。
  20. The lactase gene from kluyveromyces lactis was researched in the thesis. the cloned gene was expressed in e. coli and the properties of lactase was determinated. in addition, we studied the expression of lactase gene in the methylotrophic yeast pichia pastoris

    本論文從一株乳酸克魯維斯酵母菌中克隆獲得乳糖酶基因，在大腸桿菌中進行表達並測定其酶學性質，同時也對利用巴斯德畢赤酵母（ pichiapastoris ）系統表達該基因進行了探索。
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