cultured cell-lines 中文意思是什麼
cultured cell-lines
解釋
培養的細胞系-
2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。 -
Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants
對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。 -
We used the icr and kunming mice and got the embryos of 3. 5dpc by means of superovulation, then cultured the embryos on the feeder layer derived from icr or kunming mice, dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time. in this period, we analized the effects of feeder layer, trypsin and embryos isolated from uteri of different varietal mice on the es cell lines
以icr小鼠和昆明小鼠為實驗對象，採用超數排卵的方法獲得小鼠胚胎，培養在用絲裂黴素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上，選取適當時間離散增殖的icm和類es細胞集落，分析了在小鼠es細胞建系過程中，飼養層細胞、消化液及不同品系來源的胚胎的影響。
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