cultured cell 中文意思是什麼

cultured cell 解釋
培養細胞
  • cultured : adj. 1. 有教養的，有修養的，高尚的。2. 耕作了的；所種植的。3. （人工）培養[栽培，養殖]的。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. after cultured 48 hours, the bacilliform cell ' s surface of sporocytophaga have a great change. at this stage the bacilliform produce a lot of sticky polysaccharides. these sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium. at the same time, the filter - paper weight loss is the greatest and decomposing rate is the fastest, so we think that the sticky polysaccharides are produced during the cellulose degradation

    培養48小時，桿狀細胞的表面結構發生很大的變化，此時的菌體表面已產生大量的粘性多糖，這些粘性多糖因菌體在纖維素表面滑動而在菌體表面形成突起，即在纖維素被旺盛降解部位的菌體表面產生了大量突起；而產生突起的菌體深入到纖維素分子內部，纖維素表面可以清晰地看到由於菌體嵌入纖維素分子內部而留下的凹陷。
  2. Other spiroplasmas have been isolated and cultured on cell-free media.

    其它螺原體已被分離出來，並在無細胞的培養上培養。
  3. Es - d3 cells differentiated into ebs only when depleted of feeder cell layer by being cultured three generations in the presence of leukemia - inhibitory factor ( lif )

    結果表明： eso3細胞在含有lif的培養液中脫離飼養層培養3代以上才能形成ebs 。
  4. Environmental scanning electron microscopy ( esem ) observation of f2 mycelium cultured in liquid medium with 100mg / l of cadmium showed that there were crystalline precipitations attached to the surface of f2. transmission electron microscopy ( tem ) and energy - dispersive analysis microscope ( edam ) examination revealed that there were many granules with high content of cadmium around the cell wall

    F2在100mg l鎘濃度下培養后，經環境掃描電鏡（ esem ）觀察顯示，菌體表面有較大晶體狀沉澱物；透射電鏡（ tem ）和能譜分析（ edam ）表明，細胞壁周圍形成大量細小的高鎘含量沉澱物。
  5. Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture. the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group, while the epdermic cell were cultured simply as control. 24 hours later, under the invert microscope, the epidermic cells anchored well and transparent flat cells were observed in both groups. 7 days later, both cultured cells were taked out and fixed in 95 % ethanol, stained with hematoxylin and were observed under light microscope. many cleaved cells were observed in both groups. during cell culture, no pathogenic microganism was observed. so we considered the acellular dermal matrix was aseptic and had good biocompatibility. section three subdermal implantation of the acellular dermal matrix. 24 rats were used in the experiments. a piece of acellular dermal matrix ( 1. 5 x 1. 5cm2 ) was implanted beneath the dorsum skin flaps of each rat, 1 week, 2 weeks, 3 weeks and 4 weeks after implantation, 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured, the sections were used for he staining and observed under light microscope. the result were as folio wing : 1 - 2 weeks after implantation, the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation, the acellular dermal matrix adhered closely to the tissue around and could be recognized easily, 1 - 3 weeks after implantation, the size of implanted acellular dermal matrix had no statistical difference ( p > 0. 05 ). 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0. 05 ). under light microscope, l - 2weeks after implantation, the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix. 3 - 4 weeks after implantation, infiltrating blood vessels were evident. so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel

    結果如下：皮下包埋卜周者，無細胞真皮基質漸與周圍組織粘附，顏色由蒼白轉紅；皮下包埋3周者，無細胞真皮基質與周圍組織緊密枯附，盾晰葉辯；術后卜周，包埋的基質面積變化較包埋前無統計學差異o川0引，術后4周包埋的無細胞真皮基質面積較包埋前縮小j刃刀5 ） 。光鏡下術后卜周，宿主的淋巳組織細胞、成纖維細胞浸入生長，釉附在膠原纖維上，少量血管內皮細胞浸入基質；術后34周，無細胞真皮基質內較多的血管形成，故可認為無細胞真皮基質免疫原性低，能誘導宿主的成纖維細胞、巨噬細胞浸入生長，為一種新型的真皮替代物。第四部分無細胞真皮基質與自體斷層皮片復合移棺的研究， sd大鼠10隻，在其背部卜方造成全厚皮膚缺損的創面
  6. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  7. 2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage

    Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。
  8. Inhibitory effect of quercetin on cultured human lens epithelial cell in vitro

    槲皮素抑制體外培養的人眼晶狀體上皮細胞生長的研究
  9. Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly

    方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。
  10. Cytoskeleton of a cultured epithelial cell. microtubules are shown in green and dna is shown in blue

    處于有絲分裂間期的動物上皮細胞。綠色為微絲，紅色為肌動蛋白，藍色為染色質，中心點復製成兩個。
  11. Cytoskeleton of a cultured epithelial cell. microtubules are shown in green, actin is shown in red and dna is in blue. image by steve rogers

    人工培養的動物上皮細胞，目前處于分裂間期。綠色為微絲，紅色為肌動蛋白，藍色為dna （染色質） 。
  12. The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis

    本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。
  13. The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium

    聯合evans和martin的方法，稍加改良來分離小鼠胚胎幹細胞，把昆明白小鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上，在含有brl條件培養基的es細胞培養液中培養。
  14. At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage

    在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化，結果表明，分裂期細胞總體熒光強度強于間期細胞；而對同一細胞內熒光強度的分析說明，間期細胞的熒光主要分佈於胞質中，細胞核內則分佈較少；而正在進行有絲分裂的細胞內熒光主要集中於赤道板處；剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。
  15. The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum

    應用胰酶消化法進行頜下腺細胞的分離、純化及原代培養、傳代培養。
  16. In cultured neonatal rat cardiac myocytes, mtt methods, total protein measurement and 3h - leucine incorporation were used to evaluate the cell number and protein synthesis of cardiac myocytes

    脯氨酸摻入實驗測定膠原合成速率，放免法測定細胞內cgmp和。 amp ，定量pcr技術研究vnp對cfs的npr c的調控。
  17. A rotary cell culture system was designed, which was composed of micromotor, rotate part, gear part and et al. in this system, tubular scaffold can rotate slowly around own major axis. by means of this, three - dimension tissues or organs can be cultured in vitro

    我們還特別設計了旋轉培養裝置，通過微型馬達驅動，減速裝置作用使旋轉部分繞自身中心水平軸做緩慢轉動，從而使置於旋轉部件上的管形支架繞其正中長軸作緩慢旋轉，其原理與美國genzrpe公司的rccs （ rotarycellculturesystemrccs ）系統相仿。
  18. The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein, with mw of 34 kd. the highest amount detected with elisa reached 65mg / l medium / 105 cells. the recombinant hlf protein has the effect of inhibiting e. coli proliferation, whose activity is 1. 4 - 1. 8 times higher than the commercially available hlf

    誘導后，培養液上清通過western - blotting分析證明，轉染細胞表達並分泌出人乳鐵蛋白，分子量為34kd ； elisa檢測重組蛋白最高表達量為65mg l培養基10 ~ 5細胞；抗菌實驗表明，所獲得的重組人乳鐵蛋白具有抑制大腸桿菌生長的作用，而且比人乳鐵蛋白標準品作用更強。
  19. Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants

    對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。
  20. Furthermore, our preliminary evidence shows that the ecbp21 protein influences on proliferation of this cultured cell ( mao et al. 1999 )

    生理實驗初步表明ecbp21影響白芷懸浮培養細胞的增殖（毛國紅等， 1999 ） 。
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