directed mutation 中文意思是什麼
directed mutation
解釋
定向突變-
The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。 -
After absorption, the all - frans - retinal isomerizes to a 13 - c / s configuration and br undergoes a photocycle : br570 k590 l550 m410 n520 o640 br570 bacteriohodopsin is a promising biological photoelectric material. we intent to conduct a more thermostable br by site - directed mutagensis. a point ( no. 274 t, no. 274 a turn to c g ) mutation was introduced to br gene by successive pcr technique, and cloned into puc - 19 vector
本論文利用連續pcr的方法定點突變了br基因的一個氨基酸，突變位點選擇了br基因第273的t和274位的a ，把它們變為cg （ tyr替換為arg ） ，然後把突變的br基因克隆入嗜鹽菌表達載體pnov - r ，經測序鑒定的表達載體轉化br缺陷的嗜鹽菌，構建了tyr79 argbr突變體。
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