dna fragment 中文意思是什麼

dna fragment 解釋
限制酶切片毆
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • fragment : n. 1. 碎屑，碎片，破片，斷片。2. 未完稿，斷簡殘篇。vi. ,vt. （使）成碎片，（使）分裂。
  1. We obtain two recessive monogenic salt - tolerance mutants from co60 - - mutagenized arabidopsis thaliana m2 populations. the effect of nacl on the structure of vegetative organa in arabidopsis thaliana was further studied and through a rapd analysis on salt - tolerance mutants of arabidopsis thaliana, a 1200bp dna fragment probably related to the salt - tolerance gene was get

    本實驗以模式植物擬南芥（ arabidopsisthaliana ）為材料獲得了隱性單基因抗鹽突變體，並研究了不同濃度的nacl脅迫對擬南芥營養器官結構的影響，進而通過對突變體的rapd分析，獲得了一個與植物抗鹽性有關的1200bp大小的基因片段。
  2. Promoter function study of rm07 dna fragment in halophilic archaea

    片段在嗜鹽古生菌中的啟動子功能研究
  3. Two plasmids, which contain hbv specific dna fragment and hsv1 dna fragment, were amplified by solid phase two loci pcr and detected by enzymatic indicator system on a gene chip that was constructed by primer immobilization and modified with thiol group on chip surface. for building detection technology by dna chip in clinical, the virus genomes were extracted from the clinical positive samples by one - step nucleic acid extraction

    採用已建立的晶元制備方法，在該六種質粒中選擇含有hbv與hsv1兩種病毒dna序列的質粒為模板，進行同相兩重pcr ，採用晶元酶學槍測對固相兩重pcr的擴增結果進行檢測，得到良好的晶元酶學檢測結果。
  4. Cloning and sequence analysis of nosema bombycis s special dna fragment

    片段的克隆與序列分析
  5. So mf - 14 was believed to be a newly specific dna fragment, according to which, in the fertile line in " aijiaohuang "

    分析結果表明mf - 14是可育系中發現的一個新的特異片段。
  6. The efficiencies of hsa and hfenl integration have no mutually influences, and both can be improved by the uncoding large dna fragment

    Hsa基因與hfeni基因在小鼠體內的整合相互之間基本無影響。
  7. The minimum region of dna fragment required for an expression of red fluorescent light could be reduced to 1. 5 kb

    其中有一個亞克隆（稱為red - sac - sal ）盡管僅含1 . 5kb左右原插入片段，仍然能在紫外線下發射紅色熒光。
  8. Four out of 150 random primers could detect dna polymorphism between the two mutants and the wild type arabidopsis thaliana, which provided a strong evidence for the truth of the mutants. only one primer gave a specific dna fragment about 1200bp which two mutants own but the parent do n ' t. we drew a preliminary conclusion that the fragment might be related to the salt - tolerance gene

    擬南芥抗鹽突變體的rapd分析結果表明， 150條引物中有四條引物在突變體和野生型擬南芥之間擴增出多態性，證明了dna水平突變的發生，其中1條引物在突變體的擴增產物比在野生型的擴增產物多出一個大小約為1200bp的片段，初步認為該片段可能與植物的抗鹽性有關。
  9. It is possible that exogenous dna fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level

    這可能是外源dna片段整合進受體的基因組中，改變基因的順序或者引起基因堿基的缺失、插入，從而在基因水平上發生突變。
  10. 705bp dna fragment of mxnrampl gene and full cdna of mxlrtl gene which were related to resist iron stress were cloned by using malus xiaojinensis cheng et jiang - the first iron - efficient genotype in the genus malus in the world as material. ( 1 ) using fragment of nramp gene from wheat and fe ( ll ) - transporter gene fragment of maize ( zmlrt ~ ) as probes, we analysed these genes by blotting hybridization technique in malus xiaojinensis cheng et jiang

    本實驗以中國農業大學園藝植物研究所篩選到的一個蘋果鐵高效基因型? ?小金海棠（ malusxiaojinensischengetjiang ）為試材，分別克隆了小金海棠的抗缺鐵相關基因mxnramp1基因的752bp基因組dna片段和fe （ ） -轉運蛋白基因（ mxirt1 ）的cdna全長，為深入探討小金海棠抗缺鐵的分子機理奠定了基礎。
  11. A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome. the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 ". conditions used for the pcr consisted of 30 cycles of 94 ? for0. 5min, 45 ? for imin and 72 ? for 1. 5min, plus one additional cycle of 72 for 10min

    根據已發表的乳鏈菌肽前體基因的dna序列，設計兩條引物並引入酶切位點。以lactococcuslactisr5基因組dna為模板， pcr反應條件： 94變性30s ， 45退火60s ， 72延伸90s ，反應進行30個循環。成功擴出一條約460bp的dna片段。
  12. Labeling tunel method. the cell ultrastructural changes were similar to apoptosis in animal cells : the apical meristemetic cells underwent the programmed cell death. this was first detected in the apex cells of apical meristem, while peripheral cells differentiated gradually into different parts of a floral bud. but all the cells in the floral bud were subjected to the pcd process before it developed into a complete flower. 140bp dna fragment was found to deposit in apical bud during the plant development. the most important role of caspase - 8 was detected by western blot, and the expression of the procaspase - 8 was time - related with the dna frgmentation and the transformation from vegetative to the reproductive growth. these results suggested that pcd was an active process during the differentiation of apical meristem, and the senescence observed in the apical bud was due to the pcd process

    顯微超微結構研究表明，短日照條件下豌豆頂芽的衰老過程是從營養生長錐向花芽的轉化，而用dna原位末端標記tunel caspase - 8 western blot和140 bp dna片斷積累的試驗結果證明，轉化為花芽的整個生長錐細胞發生了編程性死亡pcd ，而且其最頂端部分細胞首先發生pcd ，而頂端周圍的分生組織細胞逐漸分化出花芽的各部分，但頂芽最後並沒有發育成為完整的花，所有細胞就都發生pcd ，從而頂芽衰老。
  13. The activity of sod could increase as the longer of storage time no matter the samples covered with vegetable oil or not, kept in light or in dark. in the pcr reaction, a dna fragment with about 550pb length has been amplified by using the genomic dna of s. maltophilia 276 as the temperate, and by using the sequ

    根據報道的不同細菌的mnsod基因序列，以5 』一tacgaygcsctggarccg弓』序列作為上游引物，以5 』一ccagttcaccacgttcca於3 』序列作為下游引物，利用pcr反應從及胭1t叩hj1z 』 a276菌株的基因組dna中擴增出一條長度約為550bp的側a片段。
  14. Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5

    Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。
  15. Therefore, research on cholesterol oxidase has become more and more important. a 1. 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi. 4 - 2g2

    本研究採用pcr方法從分泌膽固醇氧化酶的馬紅球菌菌株4 ? 2g2基因組dna中，擴增出特異dna片段，該擴增片段全長約1 . 6kb 。
  16. The amplified dna fragments were inserted into pgem - t easy vector and sequenced. the dna fragment sequencing results from the two subspecies were compared to detect whether there was any difference

    將pcr擴增產物克隆到pgem - teasy載體，進行dna序列分析，並用生物信息學方法比較東方田鼠長江亞種與指名亞種之間該序列的差異。
  17. Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence

    以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。
  18. A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing

    提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。
  19. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  20. Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science

    為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。
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