dna form 中文意思是什麼
dna form
解釋
脫氧核糖核酸形態-
The most common form of dna profile, abbreviated rflp, is a way of showing the unique patterns of bases in some of these areas
Dna剖析圖測定最普通的一種形態，縮寫為rflp ，是用來表示這樣一些區域中堿基的特有圖案的一種方法。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
With the improved method of ctab, the genomic dna extracted form plum and other species close to p. mume in genetic relationship could be directly used in pcr amplification, with good results obtained
選用改良ctab法提取的梅及其近緣種的基因組dna可直接用於pcr擴增分析，且效果良好。 -
In eukaryotic cells, dna is located in nucleolus in the form of chromatin by combining with histone proteins
在真核生物中， dna分子纏繞在組蛋白上形成染色質保存在細胞的細胞核中。 -
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。 -
Then the plasmid was extracted from them and determined by dna calculator. the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral, pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle, expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa. extract dna form injection site of mouse muscle to research time of expression
本實驗是將含grf重組質粒的jm109菌株大量培養，用堿裂變法提取質粒，用dnacaculator定量；制備含grf的原生質體，經1的戊二醛處理后注射於家兔肌肉，在注射部位給予電刺激，提取總rna ，用rt - pcr檢測grf在肌肉中的表達；用elisa法定性檢測grf在肌肉中蛋白質水平的表達；將該原生質體注射于小鼠肌肉中，定期提取dna ，初步探討原生質介導的外源性grf在小鼠肌肉中的表達時間。 -
Some junk dna could simply be spacer material that allows enzyme complexes to form around functional elements more easily
某些垃圾dna或許只是允許酶更容易合成形成周圍功能元素的中間材料。 -
According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。 -
3. its shown from the results of dna digestion, hydantoinase gene amplification, rapd, eric - pcr etc, that the electrophoretic patterns of the products are obviously different form the original one
分別提取的基因組后進行酶切分析、海因酶基因的擴增、 rapd 、 eric - pcr等檢測，兩者均呈現不同的電泳圖譜。 -
The large numbers of hydrogen bonds that form between the base pairs along a dna or rna molecule stabilize the double helix
這些互補堿基對之間形成的大量的氫鍵可以維持雙螺旋結構的穩定。 -
In the form of a double helix, dna contains the genetic blueprint of an organism
脫氧核糖核酸脫氧核糖核酸外形呈雙螺旋鏈狀，內里記錄了遺傳訊息的編碼。 -
Somehow, some of these atoms came to be arranged in the form of molecules of dna
不知怎的，這些原子中有些最後以脫氧核糖核酸dna分子的形式排列起來了。 -
They ' re a rich form of selenium, a trace mineral that convinces cancer cells to commit suicide and helps cells repair their dna
這樣的沙拉富含硒（一種礦物質元素能夠殺死腫瘤細胞並幫助細胞修復其dna ） 。 -
On the timescale that the unwanted bonds form, een a rapidly moing dna molecule would essentially appear frozen
在不必要的鍵形成的標量時間中，即使快速移動的dna分子也必須要凝固。 -
On the timescale that the unwanted bonds form, even a rapidly moving dna molecule would essentially appear frozen
在不必要的鍵形成的標量時間中，即使快速移動的dna分子也必須要凝固。 -
Dna, or deoxyribonucleic acid, is the substance ? specifically, a nucleic acid molecule with a twisted double strand double helix form ? that carries the genetic information for humans and other living organisms from one generation to the next
Dna即脫氧核糖核酸，是一種雙螺旋鏈核酸分子，它攜帶人類及其他生物的遺傳信息，使其代代相傳。 -
A complete round of transcription involves the recruitment of polymerase and general transcription factors to the promoter, rna chain synthesis initiation and polymerase escape form the promoter, rna chain elongation, and finally termination with relaease of polymerase and nascent transcript from the dna template
一個完整的轉錄循環包括ranp和通用轉錄因子被招募至啟動子、 ran鏈合成的起始和ranp從啟動子的脫逸、 rna鏈的延伸以及伴隨ranp和新生rna鏈從dna模板釋放的轉錄終止等過程。 -
Portion of mitochondrial dna control region form 39 individuals which belong to cxsp and xzsp respectively were sequenced in the study
來自宣州種群和長興種群的39個個體的線粒體dna控制區5 』端非重復序列沒有任何差異。 -
Four green plants were regenerated form resistant calli of wen. 6 derived form 1500 implanted embryos. no green plant was regenerated form calli of 200 non - transformed embryos. pcr assays of 4 green plants showed that two of them attained the expected size of amplified dna fragment ( 1500bp )
在含有20 - 60mg / l潮黴素的ms培養基上經誘導和繼代培養后，獲得了一批抗性愈傷組織，經過分化培養獲得4株再生苗，對照的200枚幼胚未獲得再生苗，對再生苗進行pcr檢測。 -
Maybe there was some simpler form of organisation, which built up dna
也許存在一些簡單的結構形式，構成了dna 。
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