dna polymerase 中文意思是什麼

dna polymerase 解釋
dna多聚酶
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • polymerase : n. 【生物化學】聚合酶。
  1. Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways

    我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。
  2. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  3. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  4. Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing

    方法利用酶切及sscp技術檢測凝固酶基因序列改變情況，並測序進行確證。
  5. Xpv dna polymerase and ultraviolet damage bypassxpc dna

    聚合酶和紫外損傷旁路
  6. The synthesized single strain oligonucleotides were separated by high performance liquid chromatography. the thermostable dna polymerase was used to catalyze snupe and the reactions of snupe for two y - snp loci were developed

    利用耐熱dna聚合酶催化單堿基引物延伸反應，建立2個y - snp的單堿基引物延伸反應。
  7. Concatemer were constructed by polymerization with t4 dna polymerase and primer - templated pcr with high fidelity dna polymerase. one concatemer of 360bp, 9 copies of grb - ast3, were obtained from the first pair of primer and three ones of 515bp, 750bp, 792bp, from the second one

    從第一對引物的拼接產物中克隆到一個360bp含有9個拷貝數的grb - ast _ 3的串聯體，從第二對引物的拼接產物中克隆到三個分別為515 、 750 、 792bp的grb - ast _ 3的串聯體。
  8. In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector

    先用t4dnapolymerase構建成模板，再通過高保真dna聚合酶（ pyrobesttmdnapolymerase ） pcr進行擴增，從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。
  9. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  10. Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis

    首先剝離大腹園蛛主壺腹腺，提取總rna和分離純化mrna ，反轉錄合成cdna ，凝膠層析除去小於400bp片段，並在雙鏈cdna兩端引入ecor的銜接頭，對其進行磷酸化處理。
  11. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  12. Pcr products were purified with qiaquick pcr purification kit, followed by sequencing using the big dye terminator cycle sequencing ready reaction kits used with amplitaq dna polymerase fs

    以pcr純化試劑盒純化pcr產物，然後進行測序反應。測序在在dna測序儀（ 377 ）上進行。
  13. The interaction of pdipl with the small subunit ( p50 ) of dna polymerase ( pol ) and the proliferating cell nuclear antigen ( pcna ) was confirmed, suggesting that pdipl may partly assist or participate in the dna replication and / or repair

    初步證實了pdip1基因能與dna聚合酶的小亞基p50和輔助蛋白pcna相互作用。因此推測， pdip1蛋白可能部分協助或參與dna的復制和修復。
  14. Comparing with using mulv reverse transcriptase or amv reverse transcriptase to do reverse transcription at 3 7 - 42 " c, more accurate and complete dna could be gotten by using tag dna polymerase at 72 c since dimeric structure of mrna was opened fully at high temperature

    與在37 - 42用mulv或amv進行反轉錄相比，在較高溫度下（ 72 ）用taqdna聚合酶進行反轉錄有利於正確、完整的cdna ，因為在高溫下mrna的二級結構被充分打開。
  15. That is to add a special fluorescence - based dna internal standard in the telomerase elongated ts primers, then do pcr amplification after a step of refinement ( hydroxybenzene / chloroform extracting, and deposited by ethanol ). sequencing analysis of pcr product was done on 310 gene scan analysis ?. 1. 2 dna sequencer to determine telomerase activity. notably, this method eliminated the restraining factors of taq dna polymerase, making it possible to erase the sample differences met in pcr and eradicate the annoying phenomena of pseudo negative results

    在kim等開發的端粒重復擴增分析法（ trap ）的基礎上進行改進，即通過對端粒酶延伸ts寡核昔酸反應產物的精製，消除了pcr擴增中抑制taq酶活性的因素，從而減少了樣品之間pcr擴增上的差異和假陰性現象的發生，提高了判斷樣品端粒酶陰、陽性的準確率和定量的準確性。
  16. Due to high sensitivity of rapd analysis to reaction conditions, main factors affecting the results including composition of the buffering system, concentrations of taq dna polymerase, primers and templates, and number of pcr cycle etc., were examined, and conditions applicable to rapd analysis of j. curcas were determined

    針對rapd標記影響因素眾多、結果重復性低的特點，對rapd分析中pcr擴增的各種條件進行了梯度測試，包括反應混合液成分、 taq酶量、引物濃度、模板濃度、 pcr循環數等。
  17. The y - a489 - plex multiplex pcr is feasible for using home product of taq dna polymerase

    Y一a489一plex體系採用的是國產的taq酶。
  18. Furthermore, the home product of taq dna polymerase had the same specificity and efficiency compared to the amplitaq gold ( pe, usa ) in this study. conclusion this is the first time that the tailed primer design protocol for multiplex pcr system is used for y - str loci

    初步構建了四個y一str基因座的y一a489一plex銀染體系和y一a4sg一plex熒光體系:並依據dna分析技術工作組（ twgdam ）指南進行了應用性研究。
  19. In this research, the expression of dna polymerase p was examined in mnng treated cells, where untargeted mutation had been proved arisen

    Lp的表達：我們用western印跡方法檢測了經mnng處理后細胞中的p 。
  20. Furthermore, we found the mutagenesis relies on the alteration on gene expression profile induced by mnng treatment ; and also, the expression of dna polymerase p ( pol p ) was proved increased after mnng treatment

    這種不依賴于dna損傷的非定標性突變隨后被證明依賴于mn 』 ng引起的細胞內基因表達的變化。進一步我們用rtpcr技術證明dna聚合酶e （ p 。 lp ）的表達在mn 』 ng處理后顯著升高。
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