eukaryotic expression 中文意思是什麼

eukaryotic expression 解釋
真核（生物的）表達
  • eukaryotic : 優核質
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  2. Construction of eukaryotic expression vector of human telomerase reverse transcriptase in immortalized hepatocytes

    構建永生化人肝細胞系人端粒酶反轉錄酶真核表達質粒
  3. Cloning of the complete coding sequence of mouse oxytocin receptor gene and its eukaryotic expression

    小鼠催產素受體基因編碼區全長的克隆和真核表達
  4. Construction of eukaryotic expression system of wild type and g88c mutant human - synuclein

    四環素負調控表達模式對肺癌細胞體外生長的影響
  5. Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system

    人骨形成蛋白3 （ hbmp - 3 ）屬于tgf -超家族的一員，可以誘導間充質細胞分化為軟骨和骨，在胚胎時期骨骼發育和骨再生修復中起著重要的作用，而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用，因而hbmp - 3有廣闊的市場前景。它在人體內含量極微，盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達，但是由於原核表達系統缺乏翻譯后的加工修飾，真核表達系統存在成本高、產量低等特點，限制了其在臨床上的應用。
  6. We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm

    Eif - 5a的hypusine修飾是其活性和功能發揮所必需的，我們通過pcr方法實現了hypusine位點的定點突變，並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。
  7. Construction of the eukaryotic expression vector pires2 - egfp - axin and its expression in glioma cells

    的構建及其在神經膠質瘤細胞內的表達
  8. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  9. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  10. Therefore, this research is the first time to attempt to express porcine ifn - a ( poifn - a ) by eukaryotic expression system

    鑒于以上研究現狀，本研究首次嘗試利用真核表達系統表達豬干擾素重組蛋白。
  11. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  12. Three dimensional structure modelling of feline ace2 and its eukaryotic expression

    貓血管緊張素轉換酶2的三維結構模擬與真核表達
  13. Construction of eukaryotic expression plasmid and pshuttle plasmid containing ctla4ig gene

    真核表達質粒及穿梭質粒的構建
  14. Construction and identification of the recombinant eukaryotic expression plasmid pvt102u - bgln

    重組真核表達質粒的構建和鑒定
  15. Construction of eukaryotic expression vector containing b7 - 1 gfp gene and its expression in osteosarcoma cell line

    融合的真核表達質粒的構建及表達
  16. 2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively

    3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。
  17. Construction and expression of recombinant eukaryotic expression plasmid of ubiquitin and epstein - barr virus nuclear antigen 1 fusion gene

    病毒核抗原1的融合表達載體的構建及表達
  18. Result 1, human antisense cd40 rna eukaryotic expression vector was constructed successfully. 2, in the presence of cd40 / pcdna3, cd40 expression was significantly decreased, cell proliferation and antibodies generation were significantly restrained, compared to that of the controls ( p < 0. 01 )

    2 、與轉染pcdna3空載體組或未轉染質粒組相比較，轉染cd40 pcdna3的健康人及sle患者b細胞系cd40的表達均明顯減少，增殖能力明顯下降， ig的分泌受到明顯抑制。
  19. Construction of eukaryotic expression vector carrying hpv16 e6 gene

    6基因真核表達載體的構建
  20. In this study, by combining rt - pcr and race, a full - length cdna encoding the lagurus lagurus zp3 ( lzp3 ) was isolated from the lagurus ' s ovary and cloned into a prokaryotic expression vector and a eukaryotic expression vector for further immunocontraceptive investigations

    本論文研究工作採用rt - pcr與race相結合的方法，首次克隆了草原兔尾鼠zp3全長cdna並進行了序列分析，構建了真核表達質粒，為草原兔尾鼠免疫不育的研究奠定了基礎。
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