eukaryotic cell 中文意思是什麼

eukaryotic cell 解釋
真核細胞
  • eukaryotic : 優核質
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. That cell hybridization can be used to dissect regulatory mechanisms controlling gene expression in eukaryotic cells.

    雜交細胞能被應用於剖析真核細胞中控制基因表現的調節機理。
  2. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  3. During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively

    提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。
  4. Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system

    人骨形成蛋白3 （ hbmp - 3 ）屬于tgf -超家族的一員，可以誘導間充質細胞分化為軟骨和骨，在胚胎時期骨骼發育和骨再生修復中起著重要的作用，而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用，因而hbmp - 3有廣闊的市場前景。它在人體內含量極微，盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達，但是由於原核表達系統缺乏翻譯后的加工修飾，真核表達系統存在成本高、產量低等特點，限制了其在臨床上的應用。
  5. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  6. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  7. Mitogen - activated protein ( map ) kinase signal transduction cascades are routes through which eukaryotic cells deliver extracellular messages to the cytosol and nucleus, and the increasing evidences showed that mapks are involved in aba -, sa - or h2o2 - signaling respectively. in addition, plant guard cells have been a well - developed model system for understanding how components interact within a signaling network in a single cell

    本實驗在表皮生物分析的基礎上，主要利用顯微注射技術、膜片鉗技術和激光共聚焦顯微技術，運用專一性蛋白激酶抑制劑處理，探索蛋白激酶對蠶豆（ viciafabal . ）氣孔保衛細胞中aba和sa誘導的h _ 2o _ 2產生及其信號轉導影響機理，結果如下： 1
  8. This course includes : the understanding of biomolecules ; the difference between prokaryotic and eukaryotic cell ; the structure of amino acids and their characteristics ; the stereo - structure of protein ; cell membrane and lipids ; the activity of enzyme ; the activity of nucleic acid ; the application of biotechnology

    本課程主要包括有生物分子之了解，真核與原核生物之異同;胺基酸之結構及性質;蛋白質與其空間構型;脂質及細胞膜;酵素之作用;核酸之作用;生物技術之應用。
  9. Construction of eukaryotic expression vector containing b7 - 1 gfp gene and its expression in osteosarcoma cell line

    融合的真核表達質粒的構建及表達
  10. Result 1, human antisense cd40 rna eukaryotic expression vector was constructed successfully. 2, in the presence of cd40 / pcdna3, cd40 expression was significantly decreased, cell proliferation and antibodies generation were significantly restrained, compared to that of the controls ( p < 0. 01 )

    2 、與轉染pcdna3空載體組或未轉染質粒組相比較，轉染cd40 pcdna3的健康人及sle患者b細胞系cd40的表達均明顯減少，增殖能力明顯下降， ig的分泌受到明顯抑制。
  11. Whereas s. cerevisiae uses irel / hacl to repress differentiation, the ire1 / xbp pathway promotes differentiation in mammalian tissues. presently, it has been found that upr signaling might promote differentiation of plasma cell in higher eukaryotic cells

    人以及小鼠胚胎組織中存在與之高度同源的est序列，說明人以及其它高等動物中可能存在大腸桿菌ibeb基因的同源基因。
  12. These work have provided us important clues to understanding biological functions of pres and hbv pathogenesis. yeast two - hybrid technique is a contemporary new method for detection of direct protein - protein interactions in a eukaryotic cell

    對于hbv前s區病毒吸附蛋白及其相應受體的研究必將為我們深入了解hbv前s區的功能及其致病機制提供重要線索。
  13. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  14. There is now strong evidence that chloroplasts and other cell organelles, such as mitochondria, represent prokaryotic organisms that invaded heterotrophic eukaryotic cells early in evolution and are now part of an indispensable symbiotic union ( see endosymbiont theory )

    這恰恰是現在有關葉綠體及一些其他的細胞器如線粒體是被原始的真核細胞吞噬進細胞內，與宿主長期共生而逐漸演化出獨立單元的內共生起源學說的重要證據。
  15. All fungi are eukaryotic organisms, and each fungal cell has at least one nucleus and nuclear membrane, endoplasmic reticulum, mitochondria, and secretory apparatus

    真菌是真核生物，含有核、核膜、內質網、線粒體和分泌裝置。
  16. Methods ; rt - pcr method was used to amplify the coding sequence of sh2a gene. eukaryotic recombined expression vector, pcdnas. 1 - sh2a was constructed and then transfected bel7402 cell and cos

    細胞激酶活性測定相關試劑二、實驗方法通過rt pcr方法擴增shzacdna編碼序列，構建真核重組表達載體pcdna3
  17. Objective : to construct the single - chain fv gene of human anti - hbsag and to analyse the expression of the constructed gene in eukaryotic cell and prokaryotic cell

    目的構建人抗hbsag單鏈抗體基因，在真核細胞和原核細胞中進行表達，並對其表達產物的活性進行鑒定。
  18. In recent years, the studies on antibiotics showed that antibiotics had significant effect on the immunoregulatory function, especially on eukaryotic cell

    摘要近年來對抗生素的研究顯示，抗生素對真核細胞功能，尤其是對免疫調節功能有明顯影響。
  19. In order to study immunization of genetic vaccine by inoculation, two kinds of dna vaccine of ndv are constructed and analysed their sequense, which will express f protein in eukaryotic cell ( hela cell ) in vitro with the method of transfection by cationic liposome the results testify that two sorts of plasmids dna successfully expressed the f protein of ndv in hela cell, but the expressed content of pc4. 0f is higher than ones of pc3. 1f

    將構建的基因疫苗pc3 . 1f大量提取和純化，採取肌肉注射和腹腔注射兩種途徑、不同劑量免疫小鼠，用elisa方法檢測小鼠血清中抗f基因表達產物的抗體水平。研究結果表明，基因疫苗pc4 . 0f和pc3 . 1f均在hela細胞中成功表達，其中pc4 . 0f的表達量比pc3 . 1f高。
  20. The precise control of the key checkpoints of the cell cycle, such as g1 / s, s / g2, g2 / m and mitosis exit, ensures the eukaryotic cells to proliferate and divide in an orderly and programmed manner

    對g1 s ， s g2 ， g2 m以及走出m期各轉換點的調控，保證了細胞周期各事件按次序正常發生，是細胞正常分裂、增殖和生長的保障。
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