eukaryotic gene 中文意思是什麼

eukaryotic gene 解釋
真核基因
  1. That cell hybridization can be used to dissect regulatory mechanisms controlling gene expression in eukaryotic cells.

    雜交細胞能被應用於剖析真核細胞中控制基因表現的調節機理。
  2. Compared to the prokaryotic gene expressing systems and mammalian gene expressing systems, insect gene expressing systems possess the stronger ability of the transcription and post - translation processing, which also has high production. we can anticipate it will be a kind of potent effective ectogenesis eukaryotic gene expressing system

    與原核表達系統和哺乳動物細胞表達系統比較，昆蟲細胞表達系統既有較強轉錄和翻譯后加工修飾能力，又有高表達量等特點，可望成為基因產業中一種比較理想的外源真核基因表達系統。
  3. We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0

    我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。
  4. Cloning of the complete coding sequence of mouse oxytocin receptor gene and its eukaryotic expression

    小鼠催產素受體基因編碼區全長的克隆和真核表達
  5. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ，並對其進行原核和真核表達，是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎，具有極為重要理論和實踐意義。
  6. During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively

    提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。
  7. This paper is a study on the expression of the protective gene of bont / a in the prokaryotic and eukaryotic. it indicates the possibility to produce the recombinant protein in quantities. it has also laid down a good foundation for the further research on vaccine or antibody of bont / a

    本論文進行了人工合成的a型肉毒毒素hc段基因在原核和真核表達系統中的表達研究，使制備大量bont a保護型抗原的重組蛋白成為可能，為進一步進行a型肉毒毒素的疫苗或抗體研究奠定了一定的基礎。
  8. Driven by the cauliflower mosaic virus ( camv ) 35s promoter, the er - shsp over - expressed constitutively. the growth and the phenotype of transgenic plants can be used for researching the function of er - shsp in improving tomato ' s cold resistance and the er - shsp chaperones function in vivo. after degested by kpnl and xbal enzymes from the pbs - er plasmid, the gene er - shsp - lehsp21. 3 was inserted into the prokii vector to construct an eukaryotic expressing vector

    利用基因工程方法，將內質網小分子熱激蛋白基因（ endoplasmicreticulum - locatedsmallheatshockproteingene ， er - shspgene ） - lehsp21 . 3導入到番茄體內，使之在植物體中組成性表達（ constitutiveexpression ）內質網小分子熱激蛋白（ er - shsp ） ，觀察轉基因番茄在低溫條件下的生長和表型反映，研究er - shsp在提高植物耐寒性中的作用，同時為體內研究er - shsp的分子伴侶機制提供依據。
  9. ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed

    （ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。
  10. Saccharomyces cerevisiae ( s. cerevisiae ) is the one ot the first and well characterized eukaryotic organisms whose complete genome was sequenced. the next chanllenge is to elucidate its gene expression pattern and the functional mechanisms of the gene products

    在所有的真核生物中，人們對釀酒酵母（ saccharomycescerevisiae ）分子遺傳學方面的認識最早，最先完成的真核生物基因組序列測定也是釀酒酵母的基因組序列。
  11. Cellular immune response after immunizing mice with eukaryotic plasmid carrying hcv hypervariable region 1related gene

    丙肝病毒高變區1相關基因免疫小鼠誘導細胞免疫反應的研究
  12. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  13. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  14. It is a good model for the study on the regulation of eukaryotic gene transcription. in eukaryotes, the acetylation of histones was discovered many years ago

    其基因表達包括基礎水平表達和誘導表達兩個層次，代表了一類轉錄調控方式，是研究真核基因轉錄調控的一個很好模型。
  15. Therefore, we hope to construct a effective eukaryotic gene expressing vector harboring a genomic dna, including introns, and develop a gene expressing system could correctly splice the mrna

    為此，我們希望構建和探索一種能有效高表達含有內含子真核基因組的載體和能對內含子進行剪切加工的昆蟲表達系統，以提高外源真核基因表達的有效性和可靠性。
  16. Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag

    目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。
  17. Construction of eukaryotic expression plasmid and pshuttle plasmid containing ctla4ig gene

    真核表達質粒及穿梭質粒的構建
  18. Construction of eukaryotic expression vector containing b7 - 1 gfp gene and its expression in osteosarcoma cell line

    融合的真核表達質粒的構建及表達
  19. 2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively

    3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。
  20. Is toxic to tenebrio molitor l. the homological cry3aa gene was modified according to the characters of poplus genes and the other features of eukaryotic gene, and was artificially synthesized. the original and modified homological c / y3aa gene were cloned into binary vector pb121 and pbin438 respectively, and transplanted into 84k clone ( p. tomentos x p. glandulossa ) to breed new anti - insects tree species

    通過對氨基酸密碼子的偏好、使用頻率、多聚腺苷酸信號序列（ ppss ） 、 mrna不穩定序列（ dst ）以及kozak等結構的優化改造，人工設計、合成了預期能在楊樹中高效表達的毒蛋白基因modifiedcry3aa同源基因； （ 4 ）利用強啟動子表達載體pet30 - b完成了cry3aa同源基因在大腸桿菌中的表達。
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