fibroblast culture 中文意思是什麼

fibroblast culture 解釋
成纖維細胞培養
  • fibroblast : n. 【生物學】纖維原細胞。adj. -blastic
  • culture : n. 1. 教養；修養；磨煉。2. 文化，（精神）文明。3. 人工培養，養殖；培養菌，培養組織。4. 耕作；栽培；造林。vt. 使有教養。
  1. There is no different between the fibroblast feeder of mouse and the fibroblast feeder of goat according to blastoysts attached and icm proliferation. es - like cell develop embryoid body - like structure in suspension culture in vitro. es - like cells of goat differentiate into various kinds cell, egg : fibroblast cell, neurolike cell

    Es細胞在體外懸浮培養，形成類胚體，山羊類es細胞在無飼養層鋪有明膠的皿底上培養，可分化為多種類型細胞，如上皮細胞、成纖維細胞、神經細胞等。
  2. Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture. the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group, while the epdermic cell were cultured simply as control. 24 hours later, under the invert microscope, the epidermic cells anchored well and transparent flat cells were observed in both groups. 7 days later, both cultured cells were taked out and fixed in 95 % ethanol, stained with hematoxylin and were observed under light microscope. many cleaved cells were observed in both groups. during cell culture, no pathogenic microganism was observed. so we considered the acellular dermal matrix was aseptic and had good biocompatibility. section three subdermal implantation of the acellular dermal matrix. 24 rats were used in the experiments. a piece of acellular dermal matrix ( 1. 5 x 1. 5cm2 ) was implanted beneath the dorsum skin flaps of each rat, 1 week, 2 weeks, 3 weeks and 4 weeks after implantation, 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured, the sections were used for he staining and observed under light microscope. the result were as folio wing : 1 - 2 weeks after implantation, the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation, the acellular dermal matrix adhered closely to the tissue around and could be recognized easily, 1 - 3 weeks after implantation, the size of implanted acellular dermal matrix had no statistical difference ( p > 0. 05 ). 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0. 05 ). under light microscope, l - 2weeks after implantation, the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix. 3 - 4 weeks after implantation, infiltrating blood vessels were evident. so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel

    結果如下：皮下包埋卜周者，無細胞真皮基質漸與周圍組織粘附，顏色由蒼白轉紅；皮下包埋3周者，無細胞真皮基質與周圍組織緊密枯附，盾晰葉辯；術后卜周，包埋的基質面積變化較包埋前無統計學差異o川0引，術后4周包埋的無細胞真皮基質面積較包埋前縮小j刃刀5 ） 。光鏡下術后卜周，宿主的淋巳組織細胞、成纖維細胞浸入生長，釉附在膠原纖維上，少量血管內皮細胞浸入基質；術后34周，無細胞真皮基質內較多的血管形成，故可認為無細胞真皮基質免疫原性低，能誘導宿主的成纖維細胞、巨噬細胞浸入生長，為一種新型的真皮替代物。第四部分無細胞真皮基質與自體斷層皮片復合移棺的研究， sd大鼠10隻，在其背部卜方造成全厚皮膚缺損的創面
  3. The concentration of fbs ( fetal bovine serum ) and ncs ( newborn calf serum ) was influential on the culture process of mef ( mouse embryonic fibroblast ) and es cells

    血清濃度的高低對胎鼠成纖維細胞和es細胞的培養過程是有影響的。無血清或低濃度的血清能抑制細胞分裂增殖，使細胞生長出現停滯。
  4. Fibroblast cell of adult mouse culture in vitro

    成年小鼠成纖維細胞體外培養
  5. ( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining

    （ 3 ）人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞（ primordialgermcells ， pgcs ） ，培養在添加人重組白血病抑制因子（ lif ） 、人重組堿性成纖維細胞生長因子（ bfgf ）和forskolin的小鼠飼養層細胞上。
  6. 6 ) after passage, the qinchuan - scalper skin fibroblast cells were in lag phase in first two day, then entered log phase and persisted six days, they stayed at plateur phase for about three days ; the mitotic index were most high at the fifth day after culture and toboggan at the sixth day

    從生長曲線可以看出，以低糖dmem培養第4代秦川牛皮膚成纖維細胞，接種后滯留期縮短，約一天左右，之後為對數生長期，持續六天左右，進入平頂期。分裂指數最高時期在培養開始后的第五天，第六天急劇下降。
  7. The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body

    小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑，其制備、傳代及冷凍保存均有不同的研究報道，飼養層的質量受許多因素的影響，如動物的品種、培養液、所傳代數及實驗條件等，關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少，故本實驗以昆明小白鼠為實驗動物，研究其胚體培養過程中細胞的形態學、組織學等方面的變化，以期為本實驗室建立飼養層細胞庫提供理論依據，同時探討心肌細胞和成纖維細胞層共培養的可行性，以期為心臟生物學特性的體外研究奠定基礎。
  8. Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast

    66弱毒株在雞胚成纖維細胞上的培養
  9. The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively

    主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
  10. At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines

    繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。
  11. The two mediums can meet primary culture and passage culture of the black bear fibroblast cells. the method of single cell cloning by micro - manipulating purifies fibroblast cells. as a result, steady fibroblast cells can be obtained and the rates of the cloning formation is 93. 75 %

    通過單細胞集落顯微法純化細胞，可克服單細胞克隆中因細胞密度太低，難以形成克隆的缺點，而且操作簡單，效率高，速度快，其克隆率可達93 . 75 。
  12. We also investigated the factors including feeder layer cells, growth factors and complements, which may affect the cultivation and differentiation of these cells in vitro. the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers

    主要內容包括： （ 1 ）小鼠胚胎成纖維細胞（ mef ）的分離培養和飼養層細胞的製作以妊娠13 . 5天或14 16天的小鼠為材料，建立了mef分離培養體系。
  13. Culture and amplification of neural stem cells of mice with serum medium containing basic fibroblast growth factor

    血清培養基輔以堿性成纖維細胞生長因子培養擴增小鼠神經幹細胞
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