flask cell 中文意思是什麼
flask cell
解釋
瓶狀細胞-
Results obtained included : ( 1 ) mscs began to attach to culture flask after 24h cell inoculation by centrifugation method. cell proliferation was active after 3d. and mscs began to attach to culture flask only after 36h cell inoculation by erythrocyte splitting method
研究結果顯示： （ 1 ）密度梯度高心法接種后24小時可見少數細胞貼壁， 3天後，細胞增殖活躍。 -
Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia
挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后，用釩鋁酸按法測定了表達產物的酶活性，結果表明重組菌株可有效表達具有生物學活性的植酸酶。 -
Cells were centrifuged for 5min at 1000rpm and removed supernatant. the cell peller was resuspended in 1 ml culture media and divided into two glass flask. cell surface makers ( cd45, cd90 ) were detected by immunocy - tochemistry
當傳到第五代時，取一瓶mscs用胰酶消化，以8x10vcm 『濃度接種在六孔板中的蓋玻片上，應用免疫細胞化學方法檢測cd45人d90的表達。 -
The vhb expression in the strain hv was investigated by co - binding assay. in flask culture, the strain hv had almost cell densities compared with e. colihms174 in the early phase of the growth
在搖瓶中培養，發現在培養前期， hv的生長與無vhb基因的對照菌e . colihms174相差不多，到培養後期時， vhb基因整合菌hv才略有生長優勢。 -
It is the foundation of therapy of hepatitis b. the gene of scfv was cloned into the expression vector pet32a ( + ) and subsequently expressed in escherichia coll. the expressed scfv fused with the 109 aa trx - tag thioredoxin protein was above 30 % total cell protein in the shaking flask cultures
融合硫氧還蛋白的單鏈抗體在大腸桿菌bl21 （ de3 ）中以包涵體的形式得到高效表達，單鏈抗體表達量達佔30的總蛋白以上。 -
Under the induction by methanol, the expressed monellin was secreted into medium at the level of 0. 25g / l. the gene ( vgb ) coding for the vitreoscilla hemoglobin ( vhb ) molecule was cloned and funtionally expressed in pichia pastoris. shake flask fermentation experiments showed that the presence of the intracellular vhb in pichia pastoris efficiently enhanced cell growth under oxygen - poor conditions
同時，採用畢赤酵母偏愛密碼子，人工合成了透明顫菌血紅蛋白基因（ vgb ） ；並構建了vgb基因的酵母胞內表達載體ppic3 . 5kv ，通過電擊轉化獲得了能在胞內表達有生物活性vhb的重組畢赤酵母gs115 / ppic3 . 5kv 。
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