fusion length 中文意思是什麼

fusion length 解釋
焊縫長度
  • fusion : n. 1. 熔解，熔化；【物理學】（核）聚變，合成。2. 〈美國〉融合；（政黨等的）合併，聯合。
  • length : n. 1. 長，長度，長短。2. （時間的）長短，期間。3. （賽艇的）一艇的長度；一馬的長度。4. 程度，范圍。5. 【板球】球程；投至適當距離的球。6. 【語言學】音長。7. 一段，一節。
  1. Aliquots of cells were mixed 0. 15 % mg / ml fb - 28, and kept at 4c for 30min, fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm. the percentages of membrane fusion was calculated. by monitoring fusion using the r18 assay, we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells

    此外，採用分子探針r18 （熒光標記物）標記病毒囊膜，體外分離中腸上皮細胞，將標記的病毒粒子與離體中腸上皮細胞混合后保溫，病毒吸附zh后，通過檢測熒光的變化來監測病毒粒子與上皮細胞的融合。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. A transcriptional fusion between 35s promoter and nearly full length hag1 cdna was constructed and transformation was performed through agrobacterium using root expantso the first whorl organs of transgenic plants flowers are carpel - like organs with stigmatic papilllae and a few ovules

    轉基因植株花的表型類似與ap2突變體，其第一輪花器官萼片具有心皮的特徵，頂端產生柱頭狀的乳突，邊緣有胚珠產生。
  4. The two cdna fragments, ap - 2a full length cdna and ap - 2 a cdna fragments were inserted in frame into gst gene fusion system. with an ap - 2a monoclonal antibody we detected the expression of gst - ap - 2a

    將這兩段篩選所得cdna片段和ap - 2 cdna全長及ap - 2 cdna的分段形式用gst基因融合系統進行表達，並用ap - 2的單克隆抗體檢測了gst - ap - 2融合蛋白表達的情況。
  5. For the welding voltage doesn ' t change, so the arc length also will not change, and the arc will penetrate into the molten puddle, which will make the swing range small, so the fusion width will decrease

    由於電壓沒有改變，所以弧廠也不變，導致電弧潛入熔池，使電弧擺動范圍縮小，就是促使容熔寬減小。
  6. 2 ) we got 55. 08 % of fusion rate when reconstructed embryos were fused with 1500 - 1800v / cm voltage and 50ms impulse. in addition, a same result can be obtained with low voltage + length impulse or strong voltage + short impulse

    41的卵母細胞達到成熟：在1500 1800v cm ， 30 40sin條件下，大鼠牛核移植重構胚能達到50廠0 55
  7. In the 317 unigenes of 592 clones, 42 novel full - length genes and 90 novel testis - specific transcripts were obtained. they were deposited in the genbank. one of testis - specific transcripts, testis - specific calpastatin ( tcast ) and two novel full - length genes, nyd - sp27 and human ubiquitin - like fusion protein ( hufp ), were selected for study

    我們對其中的一個睪丸特異的轉錄本一鈣激活的中性蛋白酶古制物（ testi specificcalpastatintcast ） ，和兩個新的全長基因一nd七p27和人泛素樣融合蛋白山umanublqultln likeusionproteinhwp ）進行了進一步研究。
  8. The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain

    成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。
  9. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  10. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  11. Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen

    本實驗從人胎盤中提取總rna ，利用rt - pcr技術獲得了編碼jo - 1的基因整長序列，選用impact - cn系統中的ptyb11載體，構建了jo - 1基因的克隆與表達載體，並轉化大腸桿菌er2566 ，經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證，篩選出了5個陽性克隆。
  12. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
  13. My main work is as following : 1 ) applying feature mapping, sub - band structure classifier and multi - classifier cooperation to enhance the robust of system ; 2 ) giving out close - set fusion and open - set fusion functions to solve the problems of speaker identification and verification respectively ; 3 ) building the dynamic recognition length algorithm based on optimal stopping rules ; 4 ) developing a applied system based on the techniques above

    主要工作是： 1 、提出參數映射、子帶結構分類器和多分類器系統以提高系統的魯棒性能； 2 、給出證據融合的閉集公式和開集公式，它們分別適用說話人辨認和確認問題的； 3 、通過最優停止理論建立識別長度自適應演算法； 4 、開發了一個實用的說話人識別系統。
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