gene sequencing 中文意思是什麼

gene sequencing 解釋
基因測序
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • sequencing : 測序，序列測定
  1. Cloning and sequencing of acc oxidase gene from sugarcane

    氧化酶基因片段的克隆與序列分析
  2. By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1

    首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。
  3. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  4. Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

    經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。
  5. Cloning and sequencing of vp3 gene of avian encephalomyelitis virus

    3基因的克隆與序列測定
  6. Cloning and sequencing of flab gene of polar flagellin of vibrio parahaemolyticus

    基因的克隆與序列分析
  7. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  8. Cloning and sequencing analysis of sus scrofa hepatitis c virus core - binding protein 6 homologue gene

    豬丙型肝炎病毒核心蛋白結合蛋白6同源基因的克隆化研究
  9. When you look at the gene sequencing that the university has done, the experience with influenza virus is that for efficient human to human spread which will then give rise to pandemic, it usually incorporates human influenza gene viruses and the analysis of gene sequence of the two cases, particularly of the brother, did not show the sequence

    雞只關在街市裡面一、兩個星期、四個星期，現時每天有數十萬雞只在街市，難免有一、兩只會感染禽流感，若這些雞只無法賣出，就有機會把病菌傳播給其他雞只。
  10. At the same time, he saw the need for a company framework in which to manufacture restriction enzymes and to develop the computing power needed for gene sequencing and predicting the properties of new enzymes

    Roberts博士還明白，要生產限制? ，以及開發電腦程式作基因排序及預測新?特性等工作，必須有一家公司支持。
  11. The dissertation consists of five chapters : in chapter one, the recent progress in molecular approaches in systematic studies of macroalgae e. g. dna extraction, restriction endonuclease fragment length polymorphisms ( rflps ), random amplified polymorphic dna ( rapd ), gene sequencing, intersimple sequence jepeats ( issr ), amplified fragment length polymorphisms ( aflp ) and single strand. conformation polymorphisms ( sscp ) were reviewed

    本論文由五部分組成：在第一部分，綜述了大型海藻dna的提取、限制性片段長度多態性（ rflps ） 、隨機擴增多態性dna （ rapd ） 、核酸序列分析、擴增片段長度多態（ aflp ） 、單鏈構象多態（ sscp ）等分子手段在大型海藻系統學研究中應用的一些進展。
  12. Laboratory tests at cdc ? including several pcr - based assays, serologic tests, electron microscopy, immunohistochemistry, and gene sequencing ? confirmed these results and showed that the virus is m # 111nkeypox virus

    Cdc的實驗室測試包括幾個以pcr為基礎的化驗、血清學測試、電子顯微鏡方法、免疫歷史化學和基因序列確認了這些結果並表明這種病毒是猴痘病毒。
  13. What is making the human microbiome project feasible is the recent development of superfast gene sequencing technologies

    使人體微生物群系項目具有可行性的是最近脂肪過多基因測序技術的發展。
  14. The study, reported in the february issue of the journal of clinical microbiology, relied on gene sequencing to compare bacteria found on the tongues of those with halitosis and those with fresh breath

    這一研究結果發表在二月的臨床微生物學雜志上。文中，研究人員是通過基因序列比較口氣有異味和無異味人群口腔內的細菌種類來論證這一結論的。
  15. Thursday, january 2, 2003 the gene sequencing analysis of the h5 virus isolated from the waterfowl at the penfold park showed that they were h5n1 virus but not the type that affected humans in 1997

    從彭福公園水禽分離出的h5病毒的基因序列分析顯示，該病毒屬于h5n1型，但是，這病毒並不是一九九七年影響人類的類型。
  16. " the gene sequencing tests showed that the h5n1 virus that was found in the waterfowl samples at the penfold park in december last year do not share the same combination of genes as the h5n1 virus that affected human beings in 1997

    漁護署發言人說：基因序列測定顯示，去年十二月在彭福公園水禽樣本分離出的h5n1病毒，其基因組合與一九九七年影響人類的h5n1病毒不同。
  17. " the afcd will continue to conduct gene sequencing tests of other samples found in birds at kowloon park and a farm in ta kwu ling, " an afcd spokesman said

    本署會繼續為從九龍公園和打鼓嶺一個雞場分離出的禽鳥病毒樣本，進行基因序列測定。
  18. Dr liu stressed that the virus isolated so far affect only live chickens. the gene sequencing tests showed that the virus was not the same as the one that affected humans in 1997

    廖季堅強調，到目前為止發現的病毒只傳染活雞，基因排列分析顯示，分離出的病毒並不是九七年禽流感影響人類的病毒。
  19. Gene sequencing on h9n2 virus shows bird to human transmission more likely

    基因排列顯示個案中病毒很可能由禽鳥傳給人
  20. Further analyses including gene sequencing is required to determine the specific type

    樣本須作進一步分析包括基因排列才可以將病毒分類。
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