hybridoma cell 中文意思是什麼
hybridoma cell
解釋
雜交瘤細胞-
Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。 -
Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained
方法:基因工程p24抗原免疫小鼠4次,歷時2個月,取脾細胞與骨髓瘤細胞株sp2 0 ,用peg融合, hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞,三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。 -
Establishment and characterization of two hybridoma cell lines secreting anti - osteosarcoma antibody
抗人成骨肉瘤雜交瘤細胞系的建立及其特性鑒定 -
Expression of human papillomavirus type 16 l1 and construction of hybridoma cell strain of human papillomavirus type 16 l1 monoclonal antibody
1蛋白表達及其單克隆抗體雜交瘤細胞株的建立 -
Development of hybridoma cell line excreting monoclonal antibody against total aflatoxins
抗總黃麴黴毒素單克隆抗體的制備及特性 -
The isolated at - iii will be used in the screening of positive clones of hybridoma cell lines
本研究採用常規法進行小鼠免疫和細胞融合。
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