hybridoma cell 中文意思是什麼

hybridoma cell 解釋
雜交瘤細胞
  • hybridoma : 雜合瘤
  • cell : n 1 小室,單室;隔間,艙;〈詩〉茅舍;(單個的)蜂窩,蜂房。2 〈詩〉墓穴,墓。3 (大修道院附屬的...
  1. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。
  2. Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained

    方法:基因工程p24抗原免疫小鼠4次,歷時2個月,取脾細胞與骨髓瘤細胞株sp2 0 ,用peg融合, hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞,三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。
  3. Establishment and characterization of two hybridoma cell lines secreting anti - osteosarcoma antibody

    抗人成骨肉瘤雜交瘤細胞系的建立及其特性鑒定
  4. Expression of human papillomavirus type 16 l1 and construction of hybridoma cell strain of human papillomavirus type 16 l1 monoclonal antibody

    1蛋白表達及其單克隆抗體雜交瘤細胞株的建立
  5. Development of hybridoma cell line excreting monoclonal antibody against total aflatoxins

    抗總黃麴黴毒素單克隆抗體的制備及特性
  6. The isolated at - iii will be used in the screening of positive clones of hybridoma cell lines

    本研究採用常規法進行小鼠免疫和細胞融合。
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