hypoxanthine 中文意思是什麼

Performance of the chinese hamster ovary cell hypoxanthine guanine phosphoribosyl transferase gene mutation assay

中國倉鼠卵巢細胞次黃質鳥嘌呤轉磷酸核糖基酶基因變異鑒定的操作

Few studies have been conducted so far on meiotic progression and none was carried out on its control via hypoxanthine in goat oocytes

但是，有關山羊卵母細胞的減數分裂進程及其調控均有待進一步的研究。

In this study, the meiotic progression and its control by hypoxanthine were studied in the goat oocytes aspirated from slaughtered ovaries

本實驗以隨機屠宰山羊卵巢為材料研究了山羊卵母細胞減數分裂的進程以及hx在這一過程中的調控作用。

2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent

取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞，再常規融合和篩選，三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。

Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed

摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。

Rat cumulus - enclosed oocyte ( ceo ) and denuded oocyte ( do ) both spontaneously resume meiotic maturation in vitro, when cultured for 2h, the germinal vesical breakdown percentage of do reaches up to 88 %, and ceos get 53 %. oocytes begin to extrude first polar body 1 ( fb1 ) when cultured for 8h. mouse oocyte spontaneous maturation in vitro can be inhibited by 4mm hypoxanthine ( hx ) or 25um 3 - isobutyl - methylxanthine ( ibmx ). while, 4mm hx can not inhibit the spontaneous maturation of rat oocyte, and the lowest effective concentration of ibmx to inhibit rat oocyle spontaneous maturation in vitro is 50um. treated with the same concentration of ibmx, gvbd percentage of rat ceo is lower than that of do, the results indicat that cumulus cells delay rat oocyte spontaneous maturation and potentate the inhibition effect of ibmx, but it ' s not the case on mouse

Ceo和do培養8h時都開始排出pb1 ；在卵母細胞成熟抑制模型中， 4mm次黃嘌呤（ hx ）和25 m的異丁基甲基黃嘌呤（ ibmx ）是小鼠卵母細胞成熟的有效抑制劑，但4mm的hx抑制大鼠卵母細胞成熟效果不理想，用hx - m199和hx - mem培養24h ， gvbd率分別為61 ， 81 ，而ibmx有效抑制大鼠卵母細胞自發成熟的最小濃度為50 m ， ibmx可以降低大鼠ceo和do的gvbd率，但ceo中的卵母細胞gvbd率要顯著低於do的gvbd率， 100 m的jbmx處理24h后，大鼠ceo和do的gvbd率分別為24 ， 84 ，提示卵丘細胞的存在可以延遲大鼠卵母細胞的自發成熟，有助於抑制卵母細胞成熟，但在小鼠上ceo和do的自發成熟差異不明顯。