identified target 中文意思是什麼

identified target 解釋
目標人物
  • identified : 經鑒定的
  • target : n 靶子，標的；目標；（嘲笑等的）對象；笑柄 （for）; （儲蓄，貿易等的）定額，指標；小羊的頸胸肉；...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Our previous work identified delayed mutation occurred at target supf trna gene in plasmid transfected into cells pretreated with low concentration of alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng )

    在收回的質粒上我們檢測到了延遲發生的、高於對照5倍以上的突變。由於w在細胞中的半壽期僅為1
  3. Feeding in this manner allows animals with superior growth rates to be identified and selected for use in the breeding herd and also ensures that these animals not in a deficit position in terms of meeting their genetically defined target body composition as they approach maturity

    按照這種模式飼喂豬，生長速度的優越性已經得到了確定，可以選擇優秀個體作為種豬群，同樣也確保在它們達到成熟時，不缺乏按照基因型定義的目標體組成。
  4. The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli

    從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e
  5. Once i have identified these phrases i start building additional pages, or even microsites ( for purposes of a / b, funnel, and conversion testing ) and i target the 3, 4, 5 or even 6 word long tail phrases

    通常來說， 「少的關鍵詞數量是好的」這是一般的概念，因為人的本性是用最小的路徑來完成他們的目標，觀點還是不變，但是人們開始使用更長的搜索關鍵字（基於我的分析） ，知道他們在找什麼，因為這樣的人群正在以明顯的趨勢增長。
  6. Results showed that : ( 1 ) in general, the segregation ratios of target gene in some tl lines are conformed to 3 : 1, however, some are not possibly due to gene silence or missing in the self - pollinated progeny ; ( 2 ) two homozygous plants were identified from 10 putative transgenic plants

    主要結果如下： （ 1 ）我們對外源基因在番茄體內的遺傳規律研究表明，在大多數情況下，單拷貝插入外源基因將導致轉基因後代按孟德爾單顯性基因3 1規律分離，但是部分轉化系比例偏低，可能發生了基因丟失或者沉默。
  7. Under single polarized measurement, wavelet transform is used to filter noise, recognition results of target are evidently raised. against the conventional definition, a new time - frequency range profile is present, thereby the more accurate features of target can be picked up and identified. secondly, methods of conductor imaging are studied

    對一維距離像的特性進行了分析；利用小波變換濾噪提高了單極化下基於目標一維距離像的識別率；相對一維距離像的傳統定義提出了時頻距離像的新定義，從而更為精確地反映目標的精密結構特徵。
  8. At present, in an attempt to stimulate specific antitumor immunity, experimental models and clinical studies are currently evaluating the potent antigen - presenting capacity of dc combined with single or multiple tumor antigen epitopes. however, there are several problems in utilizing pulsing dc with synthetic immunodominant peptides from identified antigens. 1 ) the potential induction of tolerance ; 2 ) the need to determine the patient ' s hla haplotype, the limitation of therapy to patients whose tumors express defined specific tumor antigens in the context of the correct hla phenotype, the unavailability of peptides for all hla haplotypes ; 3 ) the lack of cd4 help cell - related epitopes for most antigens ; and 4 ) the ctl resulting from such protocols have a good in vitro capacity to kill peptide - pulsed target cells but only a modest capacity to kill tumor cells

    但是，學者們發現這一療法存在著如下的缺陷：單一ctl表位抗原肽的應用其抗腫瘤作用弱於多種腫瘤抗原的聯合應用，且有誘導免疫耐受的潛在危險，有時反而會促進腫瘤的生長；事先需對患者的hla單倍型進行鑒定，以確定ctl表位與hla單倍型是否匹配，目前尚缺乏能與所有hla單倍型相匹配的ctl表位，從而限制了這一療法的應用；這一療法所產生的ctl在體外能有效殺傷經腫瘤抗原肽共孵育過的靶細胞，但對腫瘤細胞的殺傷能力較弱：這種l表位抗原肽缺乏cd4汀h細胞相關的表位，因此，不能誘導有效的th細胞免疫應答。
  9. As the target left the department store, he showed up, identified himself and invited the couple to accompany him to the icac offices in wan chai

    當目標人物從百貨公司步出時，祁國利立即上前表露身分和道明來意。面對廉署人員突如其來的出現，上市部經理夫妻兩人均感錯愕！
  10. That is the identification of material parameter is done through the true deformation increment in the loading stage before, loading increment, and the stress state by artificial neural networks, after this, we can get loading increment for the next deformation stage by the artificial neural networks trained by stress stage, target deformation increment and the identified m value

    即首先根據上一步加載材料的實際應變增量、所加應力增量以及材料所在的應力狀態識別出材料物性參數m值，然後再根據材料加載后的應力狀態、目標應變增量以及識別所得m值，由訓練好的識別應力增量的人工神經網路產生應加的載荷增量。
  11. Since submitting the self - assessment reports, most ais have already rectified the non - compliant areas identified or have set a target date to do so, " said mr william ryback, deputy chief executive of the hkma

    遵守守則。此外，認可機構提交自我評估報告后，大部份已採取糾正措施，或已擬定目標日期落實該等措施。
  12. Source - related factors, target - related factors and contextual factors are identified as antecedent constructs for alternative influence strategy choices

    本研究發現影響者相關因素、被影響者相關因素，以及系絡因素等三種因素為影響策?選擇?為之先?構? 。
  13. The expression of target protein was identified with dot - elisa, sds - page and western blotting

    Elisa 、 sda page 、 western blot等方法在蛋白水平對ctla4胞外區蛋白的表達進行了鑒定。
  14. The identifiers have the requesting entity s name inscribed so that any illegal use could be immediately identified by the target resource managers

    標識符中記錄了請求實體的名字，這樣如果有任何非法的使用，目標資源管理器就可以立即判斷出來。
  15. In this paper, the author paticular analysis 11 core holes and established the partition of strutum gyration of key layers in the area, then stratigraphic analyzed the 225 well and identified microfacies of the target area, then established the mode of facies. the author analyzed the evolvement law of different type of channel combined sedimentary environment

    本文首先通過對11口取芯井的詳細解剖，確立了研究層段的層序地層格架劃分方案，進而對研究區225口井進行了精細劃分對比，進行了不同類型河道所處環境的微相識別與劃分，並建立了研究區的相模式。
  16. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  17. Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not

    篩選tnf小分子模擬肽及結合肽對于tnf研究具有重要的意義。本研究包括以下四個方面： 1 、 tnf結合肽的研究：以rhtnf為靶對噬菌體環七肽庫進行篩選，以尋求可拮抗tnf活性的小分子短肽。
  18. In an attempt to address this issue researchers from vanderbilt university medical center identified a novel potential target for the treatment of hypertension

    致力於解決這一難題的美國范德比爾特大學醫學中心的研究人員發現了一個應對高血壓頗具潛力的的新靶點。
  19. Primary target is identified

    主目標已識別
  20. High - throughput screening depends on the development of a quantitative, pharmacologically relevant assay for the identified target, which can then be reproduced across a large number of samples

    大規模篩選有賴于對要識別的靶子的數量和藥物相關分析的發展，然後可以在大量樣本中重復。
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