industrial enzyme 中文意思是什麼

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

And regulation of stem elongation, flower inducement and seeds and fruit development are three very crucial sections in fruit industrial, so it is important to clone the gibberellins biosynthetic enzyme genes and to research on their expressing modes and their regulation roles

而莖的生長，開花的誘導，和果實種子的發育都是果樹生產中控制產量和果實品質非常重要的三個環節，因此克隆赤霉素生物合成酶的基因並研究其表達方式及表達調控在果樹生產上是非常重要的。

The continuous catalysis with a co - enzyme system based on membrane support in the membrane reactor ( mr ) is an efficient technology of co - enzyme dependent enzymatic process in industrial application

摘要在膜反應器中基於膜介質支持構建輔酶再生的酶催化體系並實現酶促反應的連續進行是輔酶依賴型生物酶工業化應用的一個有效技術手段。

Development of agricultural and industrial enzyme

農業以及工業用酵素研發

Both the ph stability and thermostabilization of evolved a - aldc were higher than those of wild - type enzyme. this mutant is more suitable for the industrial production of beer. the replacement of arg results in partial disruption of the a - helix

並對天然酶和突變酶的酶學性質進行了分析，結果表明突變酶的ph穩定性和熱穩定性均優于天然酶，更適合啤酒發酵。

Only the settle of enzyme price and purification of diacylglycerol, can the industrial production of diacyiglycerol be realized

解決好酶價格和甘油二酯的提純問題，才能實現甘油二酯低成本、高純度的工業化生產。

Xylanase refers to a type of enzyme which can hydrolyze xylans into xylooligosaccharides and d - xylose. it broadly exists in microorganism and has wide commerical application in industrial processes, such as feed, paper, foodstuff, medicine and energy industries. the xylanase gene xynb encoding the native protein of xylanase from streptomyces olivaceoviridis a1 was cloned into pichia pastoris expression vector ppic9 a

我們將來源於橄欖綠鏈黴菌a1 （ streptomycesolivaceoviridisa1 ）的木聚糖酶基因xynb的成熟蛋白編碼序列克隆到畢赤酵母表達載體ppic9中，轉化pichiapastorisgs115得到重組子，實現了木聚糖酶基因xynb的高效表達，表達產物能有效分泌且具有正常的生物學活性。

Enzymatic synthesis has wide application prospect in the industrial production of diaclyglycerol for the high selectivity of enzyme

由於酶的高度選擇性，使酶法催化生產甘油二酯具有廣闊的工業化應用前景，但使用酶法催化生產甘油二酯還存在一些問題。

Therefore, a - amylase has been used widely in many industrial fields, such as glucose production, beer brewing, fermentation trade, textile industry, and so on. the study on a - amylase is one of the most active fields in enzyme industrial fields. with the development of biotechnology, more and more scientists and researchers attempt to use dna shuffling technology to breed, screen and even " create " new a - amylase genes with higher activity and other new characters

設計引物時，上下游引物5 』端分別添加了kpn和bamh酶切位點，克隆得到的基因片段和質粒載體都用kpn和bamh進行雙酶切后，進行酶連、轉化、篩選得到陽性重組子，經過藍白斑驗證、單酶切驗證、雙酶切驗證、 pcr驗證等一系列的驗證后進行測序。