inverse pcr 中文意思是什麼
inverse pcr
解釋
反向聚合酶鏈反應-
In the past, we cloned the cdna of zmcdc5 based on the studies on difference of gene expression in shoot and radicle of maize seedling in our lab and then got the whole genome sequence by maize genomic library screening and inverse pcr
本實驗室根據玉米胚芽和胚根差異表達的研究，克隆得到zmcdc5基因的cdna ，並通過篩庫和反向pcr獲得了zmcdc5基因組全長序列。 -
In this study, we examined class 1 and 2 integron in shigella sonnei and shigella flexneri isolates from hangzhou, china, to investigate their distribution and determine their gene cassette arrays and their location in genome. a atypical class 1 integron without 3 " conserved region was found to prevail among shigella flexneri isolates, and the excision models of it ' s gene cassette were charactered by inverse pcr. moreover the roles of gene cassettes in the atypical class 1 integron and class2 integron to confer resistance were discussed
本研究對杭州地區近年分離的福氏和宋內氏志賀菌株進行類和類整合子的檢測，了解其分佈特徵；進一步通過克隆測序和southenblot ，確定其攜帶的基因盒組成特徵以及其在基因組中的位置；在研究中發現了一種缺少3 』保守區的非典型類整合子，通過反轉pcr了解此整合子的切除基因盒的特徵；並對非典型類整合子和類整合子各自攜帶的aada賦予宿主菌鏈黴素抗性的不同能力，進行了探討。 -
Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. a partial fragment of the glycine betaine transporter beth gene was obtained from the genome of h, trueperi with degenerate primers. through southern blot hybridization and inverse pcr, a 5. 1 kb ecori fragment containing the beth gene was sequenced
將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southern雜交，結果顯示在ecori酶切片段的5 . 1kb處有陽性信號。 -
In this study, using bioscien tomato as test material and the flanking region of the bioscien insert sequence was determined using inverse pcr and real - time pcr. by means of verification, this sequence is specific for the transfection event
本研究以華番1號為試材，利用反向pcr和熒光pcr技術已成功將華番1號轉基因整合位點dna序列擴增出來。 -
Dna sequences flanking tn5 - 1063 of 042bmr5 were amplified using inverse pcr, it was found that tn5 - 1063 was inserted into noeb gene
對042bmr5突變株的基因組進行反向pcr ，擴增位於tn5 - 1063兩端的側翼序列。
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