ion protein 中文意思是什麼
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Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。 -
Ion channel protein superfamily
離子通道蛋白質的超家族 -
The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method
Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。 -
Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。 -
Study on noncovalent binding between ketoprofen and plasma protein by electrospray ion trap mass spectrometry
酮洛芬與血漿蛋白非共價結合的電噴霧質譜研究 -
Layered and pillared material are a kind of multifunctional material which were developed in recent years, much attention has been paid to this kind of material for its application in ion - exchange catalysts solid state proton conductivity, nonlinear optics and physic. a lot of literature have reported the intercalation behavior of a - zirconium phosphate ( abbreviated as a - zrp ), different guest molecules inserted into a - zrp have been studied in detail, those guest molecules include amine, alcohok amino acid protein, enzyme coornadiate compound and coronal compound. the intercalation guest is restricted by their size and basicity
層柱材料是近年來發展起來的一類多功能材料，由於其在離子交換、催化、固態質子導體、非線性光學以及醫學等方面的廣泛應用而受到國內外研究者的重視，大量文獻報道了-磷酸氫鋯zr （ hpo _ 4 ） _ 2 ? h _ 2o （ - zirconiumphosphate ，縮寫為- zrp ）的超分子插層化合物及插層性能，其中對不同的客體分子對磷酸鋯的嵌入做了詳細的報道，客體分子的種類包括氨、醇、氨基酸、蛋白質、酶、配合物、冠狀化合物等。 -
Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel
融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。 -
Extracted ion chromatograms for protein chromatography based on peptide elution were generated
產生了基於肽洗提的蛋白色譜圖的萃取離子色譜圖。 -
These were shown to emulate ion chromatograms produced in a subsequent run without the digestion element, based on protein elution
可以仿效一個序列運行的離子色譜圖，而不需要基於蛋白洗提的酶解成分。 -
We purified the fusion mpt64 protein by the nickel - ion affinity chromatography
通過鎳離子親和層析獲得相對純的mpt64重組蛋白。 -
Hd biosciences co., ltd has announced an important research collaboration with organon, the human health care business of akzo nobel. the agreement relates to the development by hd biosciences of functional assays for a number of identified g - protein coupled receptors and ion channels
根據這項協議，華大天源將為歐加農公司構建一系列的g蛋白偶聯受體家族和離子通道家族的篩選模型，這些篩選模型均適應于高通量藥物篩選。 -
Cystic fibrosis is caused by mutations in the gene encoding an ion - transport protein, the cystic - fibrosis transmembrane conductance regulator ( cftr ). defective secretion of anions is the primary cause of many of the clinical manifestations of cystic fibrosis, including pancreatic insufficiency
囊性纖維化是由作為陰離子通道的跨膜電導調節器（ cftr ）的基因突變所致，而陰離子分泌缺損是囊性纖維化的多種臨床表現（包括胰腺功能缺陷）的主要原因。 -
In this paper, one interactive protein was isolated and purified further by ion - exchange chromatography, whose molecular weight was determined by sds - page to be 24kda under non - reduced conditions and 25kda under reduced conditions
最後，分離純化作用蛋白中分子量為24kda的蛋白，發現其存在鏈內二硫鍵，二硫鍵斷裂后的分子量為25kda 。 -
In conclusion, the compact engineered peptide can form lethal ion channel in the membrane of staphylococcus aureus guided by staphylococcal agrd pheromone, therefore the engineered peptide had bactericidal activity against staphylococcus aureus, especially against the drug - resistant strains. because of the properties mentioned above, the engineered peptide showed great potential in the future. the engineered peptide was an example of bactericidal protein machine combining two minidomains with different bioactivities and different protein origins
該工程多膚能在人工脂質膜上形成電壓依賴性離子通道，即當電壓由omv變為+ somv時通道立刻開放，電導驟然上升到某一數值，其後電導發生丫系列諧振式變化;而當電壓由+ somv變為一50mv時電導並不立即減小至o ，而出現正電壓狀態下類似的表現。 -
In n terminal, 3 conserved sequences, wxixgmxgxgkttla, l ( i / v ) ( v / l ) lddv ( w / d ) and sriixttrd xxv are in term of p - loop, kinase 2 and kinase 3a. those are highly identical to the homologous of human ( apaf - 1 ) and namenode ( ced - 4 ). it revealed that tm - 22 encodes a membrane protein with a receptor structure and kinase property and may function in ion flow, phosphorylation and proteins interaction
Tm一夕基因的編碼蛋白在該區與tm一2有32個氨基酸的差異，主要集中在第10 、 11和12個亮氨酸重復序列中，與tm一2有兩個氨基酸的差異，僅僅是位於第12個亮氨酸重復序列中的767和769兩個位點，這表明:第一， lrr是tm一22基因和tm一2基因編碼蛋白對病毒識別的特異區段;第二， 767和769兩個位點的差異氨基酸是tm一22基因和tm一2基因編碼蛋白對病毒識別的特異位點。 -
Our results indicated that : since s - 100 protein is a sensitive functional marker for astrocytes, the increased expression of s - 100 protein suggest that astrocytes may be involved in the pathological processes of the ion - radiation injury of brain and neural - hormone reaction of the rat
以上結果表明s 100蛋白作為腦內星形膠質細胞功能狀態的重要指標，其表達的顯著增加，提示腦內呈形膠質細胞可能參與了大鼠腦輻射損傷病理過程或神經體液反應過程。 -
These proteins involved in various cellular functions including carbohydrate transport and metabolism, energy production and conversion, inorganic ion transport and metabolism, amino acid transport and metabolism, posttranslational modification, protein turnover, chaperones, function unknown and not in cog most of the proteins identified are involved in energy production and conversion, carbohydrate and amino acid transport and metabolism, especially in energy production and conversion
這些蛋白質在細胞中行使不同的功能，主要包括: 1 .糖類轉運與代謝: 2 .能量的產生與轉化; 3 .無機離子轉運與代謝: 4 .氨基酸轉運與代謝; 5 .翻譯后修飾，蛋白代謝，分子伴侶: 6 .一些未知功能的蛋白等。在鑒定的13個蛋白中大多數是與糖類和氨基酸轉運及代謝和能量的產生與轉化相關。 -
The newly identified gene, nyd - sp27 has structural similarity to an isoform of phospholipase c. nyd - sp27 was expressed endogenously in human pancreatic - duct cells and upregulated in cystic fibrosis. researchers has proved that suppression of the gene resulted in augmentation of phospholipase - c - coupled calcium - ion release and protein kinase c activity, improvement in the amount of mutated cftr reaching the plasma membrane, and thus restoration of pancreatic anion secretion
Nyd - sp27內源性表達在人體的胰腺導管上皮細胞，並在囊性纖維化時表達增高，顯示該基因可能與病變有關。研究人員發現通過抑制該基因在人體囊性纖維化胰腺導管細胞中的表達，可以糾正缺陷的胰腺陰離子分泌。 -
The agreement relates to the development by hd biosciences of functional assays for a number of identified g - protein coupled receptors and ion channels
位於中國上海，是一家以天然產物為基礎的藥物研發和服務公司，並且在這個領域處于領軍位置。 -
It showed that eif - 5a subcellular localizat ion was a dynamic process, and it might be a nucleocytoplasmic shuttle protein. our study is the first to show that the inhibition of hypusine formation altered the subcellular distribution of gfp - eif - 5 a, and made it unable to localize at its proper site of action
然後利用氨基酸突變、添加阻斷劑等方法，抑制eif - 5a的hypusine修飾，觀察到eif - 5a始終為全細胞分佈，提示抑制hypusine的形成可影響eif - 5a的亞細胞定位，進而影響其功能的發揮。
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