membrane fusion 中文意思是什麼

membrane fusion 解釋
膜融合
  • membrane : n. 1. 【解、生】（薄）膜，隔膜。2. 〈古語〉（古文件的）（一頁）羊皮紙。-braneous , -branous adj. 膜（狀）的，膜質的；隔膜的。
  • fusion : n. 1. 熔解，熔化；【物理學】（核）聚變，合成。2. 〈美國〉融合；（政黨等的）合併，聯合。
  1. Aliquots of cells were mixed 0. 15 % mg / ml fb - 28, and kept at 4c for 30min, fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm. the percentages of membrane fusion was calculated. by monitoring fusion using the r18 assay, we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells

    此外，採用分子探針r18 （熒光標記物）標記病毒囊膜，體外分離中腸上皮細胞，將標記的病毒粒子與離體中腸上皮細胞混合后保溫，病毒吸附zh后，通過檢測熒光的變化來監測病毒粒子與上皮細胞的融合。
  2. In the 1990s, the pheromones of gram - positive bacteria, which regulates the growth and toxin secretion of the same type bacteria, were identified they were peptides consisted by dozens of amino acids. the pheromones can auto - recognize membrane receptor of the identical types of bacteria. we had constructed a fusion protein named pheromonicin by introducing a staphylococcal pheromone agrd at the c - terminal of the colicin ia

    丘小慶等利用信息素的自主導向特性和大腸菌素ia的致死性通道特性構建了由金黃色葡萄球菌信息素agrd和大腸菌素ia組成的融合蛋白，命名為pheromonicin ，該蛋白表現出了信息素和大腸菌素ia都不具有的抗金黃色葡萄球菌活性。
  3. It is formed by fusion of vesicles from the golgi apparatus, resulting in a flattened membrane - bounded sac spreading across and effectively divding the cell

    高爾基體產生的小泡在赤道面上彼此融合而形成有膜包圍的平板，即細胞板。
  4. The results suggested that monocrotophos had not only environmental estrogen effects, but also reproductive toxicity. monocrotophos could destroy the membrane systems of three kinds of pituitary secretary cell, spermatogenous cell and sperm. in three kinds of pituitary secretary cell, nuclear membrane and endoplasmic reticulum showed dilation, and endoplasmic reticulum even exhibited fusion under exposure in high concentration

    久效磷對精原細胞的損傷最為嚴重，細胞膜和核膜水腫，高濃度暴露組的細胞膜和核膜溶解，線粒體的崎也有溶解現象;但久效磷對精母細胞和精子細胞的損傷並不明顯，主要表現為膜系統的輕微水腫。
  5. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  6. The results were as follows : ( l ) the pre - incubation of pmr induced the exocytosis of the cortical granules ( cgs ) in the oocytes and reduced the quantity of the microvillus on the surface of oocytes ; ( 2 ) the matrix of cg might migrate to the surface of plasma membrane of oocytes after pmr pre - treatment. ( 3 ) incubation of zona - free hamster eggs with spermatozoa pretreated with pmr a ntiserum was associated with a dose - dependent significant reduction in the number of spermatozoa binding to the oolemma, compared with a contraband with a dose - dependent reduction of the fertilization rate and the penetration index. the results indicate that mr of human sperm plays an important role in sperm - oocyte fusion and oocyte activation

    結果顯示： （ 1 ）人精子mr能夠誘發去透明帶金黃地鼠卵母細胞中皮質顆粒的胞吐，並引起卵母細胞膜表面微絨毛形態和數量發生改變； （ 2 ）皮質顆粒胞吐后，其內容物可結合到卵膜表面； （ 3 ）抗mr抗血清預處理精子可抑制精子與卵子的結合與融合，使受精率、穿透指數和結合指數下降，並均有劑量依賴性。以上結果提示，人精子mr在受精過程中起重要作用，其主要作用可能是介導精-卵膜融合併促進卵母細胞的活化。
  7. The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316

    Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。
  8. There are so many molecules involved the fertility, such as : spl7, a mammalian testis - and sperm - specific protein, can specifically bind with zona pellucida, dextran, dextran sulfated, and fucoidin. another germ cell cyritestin is a testis - and sperm - specific protein belonging to the adam family. it has a disintegrin domain at the n - terminal, known as a integrin ligand, that suggests cyritestin could be involved in sperm adhesion and fusion with the egg membrane previous study demonstration

    配子中的許多分子均參與了上述關鍵環節，如在精母細胞、精細胞和睪丸精子中表達，並特異性與卵子透明帶糖蛋白、葡聚糖、糖酐脂結合的精子表面蛋白17 （ sp17 ） ，以及參與識別或與卵子膜上的整合素相互作用的adam家族成員之一的cyritestin蛋白等。
  9. The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain

    為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
  10. By yeast two - hybrid assay, aes was found to interact with gp130 intracellular region through its conserved q domain. results from the yeast two - hybrid assay, gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain, and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction. to investigate the consequence of this interaction, tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector

    在通過酵母雙雜交分析確定aes通過q結構域與sp130分子胞漿區結合的基礎上，為確定tle1分子是否也能通過保守的q結構域與gp130分子胞漿區結合，我們通過pcr擴增編碼gp130胞漿區與tleq分子不同結構域的cdna ，構建了含有這些不同結構域的酵母雙雜交載體，通過酵母雙雜交分析證實： tle1分子通過其氨基端的q結構域與gp130分子胞漿區近膜段結合。
  11. The protein is known as fusion protein and is found on the outer surface of parainfluenza virus 5, a so - called " enveloped " virus that fuses its membrane with the membrane of its host cell before infection

    這種在「副流感病毒5型」的外層表面發現的蛋白質通常被叫做「融合蛋白」 fusion protein ，即所謂的「包膜」病毒，它可以將自身細胞膜與人體的細胞膜進行融解並感染。
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