newcastle disease virus 中文意思是什麼

newcastle disease virus 解釋
新城雞瘟簿
  • newcastle : n. 1. 紐卡斯爾〈澳大利亞港市〉。2. 紐卡斯爾〈英國港市〉。
  • disease : n. 1. 病，疾病；【植物；植物學】病害。2. （精神等的）病態，弊病。3. （酒等的）變質；（食物等的）腐敗。4. 〈廢語〉不安。
  • virus : n 1 【醫學】病毒；濾過性病原體。2 毒素；毒害。3 惡意，惡毒。4 【計算機】（電腦）病毒〈指擾亂或破...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. In recent years, the outbreak of nd appear some new characters, it is reported that the amount of immunity defeat on nd increased, even chicks with high hi titer can be infected by ndv virulent strain. the gene - engineering vaccine is badly needed to control nd outbreak. so newcastle disease virus strain b95, a naturally avirulent and heat stable strain, was introduced from australia

    新城疫是國際獸疫局公布的16種烈性傳染病之一。近年來，新城疫的爆發出現了許多新特點，免疫失敗報道增多，甚至抗體水平較高的雞群也發病。為控制新城疫的爆發，急需研製基因工程苗。
  3. From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain

    本實驗採用spf雞胚及雞胚原代成纖維細胞，從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞，並且這種凝集可以被特異性抗血清所抑制。
  4. The investigation on the molecular epizootiology of newcastle disease virus

    中國部分地區新城疫病毒的分子流行病學研究
  5. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  6. Newcastle disease is a highly contagious and economically most important disease of poultry. the disease also inflicts many other avian species. the disease varies in degree of severity ranging from mild, inapparent infecion to severe disease. based on the severity of the disease in chickens, the causative agent of the disease, newcastle disease virus ( ndv ), is divided into three pathotypes : ( 1 ) the lentogenic strains, which cause mild inapparent infection ; ( 2 ) the mesogenic strains, which cause mild respiratory symptoms with low mortality ; ( 3 ) the velogenic strain, which are highly virulent and cause high mortality

    新城疫（ newcastledisease ， nd ）是由新城疫病毒（ newcastlediseasevirus ， ndv ）引起的侵害禽類的急性高度接觸性傳染病，是家禽傳染性疾病中危害最嚴害的疫病之一，由於對世界養禽業危害極大，故被國際獸疫局確定為類傳染病。在傳染性疾病的預防中，疫苗是減少易感人群而被最廣泛採用和最有效的途徑。
  7. Construction of baculovirus transfer vectors for expression of core antigens of newcastle disease virus

    核心抗原重組桿狀病毒轉移載體的構建
  8. Twenty years later scientists found that a virus that causes the veterinary disorder newcastle disease shows a preference for infecting tumor cells and began to try to enhance that tendency by growing the viruses for generations in human cancer cells in laboratory culture dishes

    20年後，科學家發現引起新城雞瘟的病毒，特別偏愛感染癌細胞，因此他們在實驗室里的人類癌細胞株中培養此種病毒，試圖加強這種傾向。
  9. Inspection method on newcastle disease virus in poultry for export

    出口家禽新城疫病毒檢驗方法
  10. The causative agent of the disease, newcastle disease virus ( ndv ), is a member of the paramyxoviridae whose genome is a non - segmented single - strand negative - sense rna

    該病的病原新城疫病毒（ newcastlediseasevirus ， ndv ）屬副粘病毒科，為有囊膜的負鏈rna病毒。
  11. Newcastle disease virus ( ndv ) is grouped under the family of paramyxoviridae, it causes serious infectious disease and incurred great losses to poultry industry. f and hn protein were main glycoproteins, which affect the virulence of the virus

    新城疫病毒（ newcastlediseasevirus ， ndv ）屬于副粘病毒科（ paramyxoviridae ）副粘病毒亞科（ paramyxovirinae ）腮腺炎病毒屬（ rubulavirus ）的成員，其主要結構蛋白融合蛋白（ f ）和血凝素-神經氨酸酶（ hn ）與其致病性密切相關。
  12. Avian paramyxovirus type 1 ( apmv - 1 ) may cause diseases, named differently in different poultry species, including chicken newcastle disease, goose paramyxovirus disease, pigeon paramyxovirus type 1 disease. the virulence and pathogenicity of apmv - 1 field isolates are different among the poultry species. there is not a technique available for the rapid diagnosis of the disease in all the species and the determination of virulence of the virus at the same time

    禽型副粘病毒（ avianparamyxovirustype1 ， apmv - 1 ）引起的相關疾病主要有雞新城疫、鵝副粘病毒病、鴿型副粘病毒病，但不同禽源的apmv - 1分離株對其它禽類的毒力和致病性都有所差異，雖然apmv - 1在多種禽類廣泛流行，目前仍未有一種既可檢測所有禽源的apmv - 1 ，同時又能鑒別其毒力強弱的快速診斷方法。
  13. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  14. Newcastle disease virus

    新城疾病病毒
  15. A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses

    依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
  16. Exotic newcastle disease spreads fastest among birds kept close together. the virus is spread through bird droppings and fluid from the nose, mouth and eyes

    異國情調新城疫蔓延最快的鳥保持著密切合作。該病毒的傳播是通過鳥的糞便和液體從鼻子，嘴和眼睛。
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