opposite sequence 中文意思是什麼
opposite sequence
解釋
逆程序-
In light of the simulative research results, three methods are applied to analyze some controversial problems in this kind of structural system ( such as the concrete pouring sequence of continuous ends, the applying sequence of pretensioned force, the removal of the temporary supports, the concrete pouring style of continuous ends, the optimization of constructional reinforcement and prestressed reinforcement ). some important conclusions are drawn : the concrete pouring sequence of continuous ends should be in accordance with the principle of " interval end " ; the removal of temporary supports is also on this principle ( opposite to traditional opinions ). at the same time, other problems such as the shrinkage and creep effects of concrete, the c racking and failure pattern, the optimization of reinforced bars and prestressed bars are also analyzed in detail
在此基礎上，對于目前先簡支后連續結構體系中存在的一些有爭議的問題（如后連續端部澆築和后連續預應力張拉的順序、體系轉換中的臨時支座拆除順序、后連續端部澆築方式、后連續端部的預應力筋及普通鋼筋的優化等） ，我們分別運用三種方法進行了細致的模擬分析、研究，並得出了一些具有重要工程意義的結論：后連續端部的澆築順序和后連續預應力張拉的「隔跨」原則，臨時支座拆除的「隔跨」原則（此前人們一直認為「對稱澆注和對稱張拉」是最為合理的施工工序） ，混凝土的收縮、徐變對先簡支后連續結構體系的影響一般較小等。 -
And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。
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