pcr cloning 中文意思是什麼

pcr cloning 解釋
pcr克隆法
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  • cloning : 基因轉殖 克隆
  1. Cloning of ctsox2 gene shows the validation of rpgw - pcr used for isolating genes from the digested genomic dna

    C心以2基因的hmg盒區外的序列信息可以進一步研究它的功育旨。
  2. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  3. Then the pcr product was purified, ligated into pgem - t vector by ta cloning

    篩選陽性克隆，測序，大量制備序列完全正確的質粒。
  4. Described here is the cloning and characterization of a new trichome - specific - promoter in arabidopsis. by ddrt ( differential display reverse transcription ) - pcr and reverse northern, a 280bp sequence is acquired from the epidermal in viciafaba, which is specially expressed in the epidermal. then using race, we abtain a 3. 0 kb gene segment including the 3 ' end full sequence and the 5 ' end partial sequence of the 280bp segment

    首先，我們利用差異顯示技術和反northern技術，以蠶豆葉肉細胞為對照，從蠶豆葉片表皮細胞中克隆出長280bp的新的表皮特異表達基因片段，再通過race技術獲得此基因片段的3 』端全序列和5 』端部分序列。
  5. Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process

    本論文在前人研究的基礎上，開展了以下兩個方面的工作： （ 1 ）採用分子生物學技術熒光原位雜交（ fish ） 、多聚酶鏈式反應（ pcr ） 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測； （ 2 ）探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性，為天然底質環境中厭氧氨氧化過程的強化，富營養化底質微生物修復的可行性提供一定的依據。
  6. In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector

    先用t4dnapolymerase構建成模板，再通過高保真dna聚合酶（ pyrobesttmdnapolymerase ） pcr進行擴增，從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。
  7. The full - length cdna of hbrp is amplified by pcr reaction and sequenced after cloning. 3

    在序列的兩端設計引物， pcr擴增全長cdna ，克隆后測序分析。
  8. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  9. In our study we have cloned the osd gene from s. typhimurium by pcr, characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd

    再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質粒的多克隆位點之內。
  10. Firstly, the hyaluronate synthase ( has ) gene ( hasa ) was cloned into the cloning vector puc19 by pcr using the total dna sample of s. equi as template

    本文研究代謝工程理論，對本實驗室的一株ha生產菌（ streptococcusequi ）進行了ha合成與分解關鍵酶基因及功能的研究。
  11. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  12. Arc1, thl1 and thl2, the substrate protein genes of s receptor kinase, were cloned through a series of methods of molecular biology such as pcr, rt - pcr, dna cloning and sequencing. the resultings sequences were highly analysed by using the related biosoftwares on internet, providing new insights in the field of the molecular mechanism of self - incompatibility in plants. the major results are as followings : 1

    本文通過pcr和rt - pcr等一系列分子生物學方法克隆了蕓薹屬植物中的甘藍和油菜自交不親和信號傳導過程中srk底物蛋白基因arc1 、 thl1和thl2 ，並使用各種相關生物信息學軟體對srk底物蛋白基因序列進行了分析，然後在internet網上利用在線軟體對蛋白質的結構和功能進行了預測和探討，以期為蕓薹屬植物自交不親和性的分子機理的研究提供新的內容。
  13. At last, ctab - dna and sds - dna methods are used in this experiment. on the basis of optimizing experiment procedure and pcr system of the materials, the initial reversion transcription system and orthologus gene cloning technique are established

    對這3種方法比較后，確定本實驗採用ctab - dna酶消化法和sds - dna酶消化法提取白樺雄花芽組織的總rna 。
  14. 2. cloning of structural genes of bacillus subtilis bio operon diluted the genomic dna of bacillus subtilis as the template, long pcr product ( 10. 3kb ) and three salvage pcr products were separately gained by optimization of reaction conditions of pcr

    枯草桿菌生物素操縱子基因的克隆將枯草桿菌基因組dna稀釋后，通過pcr反應條件的優化，分別擴增得到了生物素操縱子基因的長距離pcr產物（ 10 . 3kb ）和3個分段pcr產物。
  15. 2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence

    異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。
  16. In this experiment, ri gene was amplified by pcr method from recombinant cloning vector pt7 - ri, which had been constructed by dr. yu xiu - ping in this laboratory and sequenced by takara biotechnology ( dalian ) co., ltd.

    于秀平博士已經構建了ri的克隆載體pt7 - ri ，測序表明與已知人胎盤ri基因的cdna序列完全相同。本文的工作是在此基礎之上完成的。
  17. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  18. Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe

    對片段進行克隆、亞克隆之後測序分析，發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性；並且三次獨立的pcr均得到相同的片段，所克隆的基因序列與choe相比，連續缺失三個堿基（ ttc ） ，即相應的氨基酸序列缺失一個氨基酸（ phe ） ，因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然突變體，命名為choew 。
  19. 3. the cloning of a - amylase gene : the methods of shotgun, pcr and rt - pcr were selected to clone a - amylase genes from bacillus subtilis hn503, xanthomonas campestris pv. campestris 8004 and aspergillus oryzae, hn504 the recombinant plasmid with cloned gene was designated as phn504, phn503 and phn8004

    本研究分別選用了鳥槍克隆法、 pcr和rt - pcr克隆法，成功地克隆到枯草芽胞桿菌（ bacillussubtilis ） hn503 、野油菜黃單胞菌（ xanthomonascampestrispvcampestris ） 8004和米麴黴（ aspergillus口盡留『 ） hn504的價澱粉酶基因。
  20. Includes catalog of research products, vector maps and sequence data, product manuals and brochures, and forums for gene expression and pcr cloning

    -主要產品有包埋式生物標本，生物標本及實驗儀器試管標本教學儀器玻璃儀器等
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