pcr-sscp 中文意思是什麼
pcr-sscp
解釋
聚合酶鏈反應單鏈構象多態-
Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing
方法利用酶切及sscp技術檢測凝固酶基因序列改變情況，並測序進行確證。 -
Pcr - sscp analysis on partial sequence of gonadotropin releasing hormone receptor gene in sheep
3基因外顯子2的克隆與序列分析 -
Pcr - sscp analysis on exon 1 of myogenin gene in sheep
側翼調控區的克隆與序列分析 -
But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation
將表達產物破包涵體后經glutathinonesepharose4b柱親和層析分離， sds page顯示純化的蛋白質為一分子量60kd的單一蛋白帶。 -
With strong specificity and easy to manipulation, this simple, inexpensive, rapid molecular beacon hybridization technique permits visual monitoring of gene point mutation and snp, which shows better advantage than pcr - sscp. 2
Mb雜交技術特異性強，簡單快捷，能進行可視性檢測，完全適用於點突變和snp的研究，技術特點明顯優于pcr sscp 。 -
By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ), the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated. 3. the molecular beacon ( mb ) hybridization technique with visual monitoring was established
建立聚合酶鏈反應單鏈構象多態性分析inglestrandconforma tlonpolymorphlsmanalysisofpol仰erasechainreactionproduction ， pcr sscp ）方法，初步調查漢族人群mbl基因ggc54gac突變情況。 -
The application of pcr - sscp in forensic mtdna typing
分型的法醫學應用 -
Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。 -
This research began with detecting the rpob gene mutations of 37 mycobacterium tuberculosis clinical isolates by polymerase chain reaction - single strand conformation polymorphism ( pcr - sscp ) to study the application value of this method as a new molecule testing method for drug susceptibility
本研究首先通過聚合酶鏈反應?單鏈構象多態性分析技術對37株結核分枝桿菌臨床分離株的rpob基因進行突變檢測，目的在於探討pcr - sscp作為新的分子藥敏試驗方法的應用價值。 -
Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k. a 109bp fragment was amplified from the human genome dna. 2. in 166 han samples, 19 ( 11. 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
用紅細胞裂解液和pcr緩沖液非離于去污劑蛋白酶k兩種自配試劑成功地提取了所收標本的人白細胞基因組dna ，並以此為模板成功擴增出預期的109hpmbl目的基因片段。 -
Compared with the results of dma direct sequencing, pcr - sscp has the same results as that except for failing to check out the rpob gene mutation of a rifampin - resistant isolate. it proves that pcr - sscp is a rapid and sensitive mutation testing method
與dna測序結果相比較， pcr - sscp除一個耐藥株的rpob基因突變沒有檢出外，其它檢測結果均與dna測序結果相一致，這進一步說明pcr - sscp是一種快速、靈敏的突變檢測技術。 -
To investigate the distribution of gene mutations in lpl and to study the relationship between lpl mutations and plasma lipid, the lpl gene ( exon5 ~ 9 including intron - exon boundaries ) was examined by pcr - sscp analysis, and the amplified products showing abnormal pattern on sscp were sequenced using dideoxy - mediated chain - termination
在外顯子5中，檢出一雜合子突變，經dna序列測定證實為pro （ 207 ） leu突變雜合子，該患者為高甘油三酯血癥病人。 -
Polymorphism of brucella omp28 analyzed by pcr - sscp
多態性分析 -
The research results further prove that resistance to rifampin in mycobacterium tuberculosis resulted from the rpob gene mutation and pcr - sscp can quickly detect rifampin resistance in mycobacterium tuberculosis clinical isolates
這些研究結果進一步證實，結核分枝桿菌耐利福平是由於rpob基因突變所致，採用pcr - sscp可快速檢測結核分枝桿菌對利福平的耐藥性。
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