positive segment 中文意思是什麼
positive segment
解釋
正向地段-
The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling
利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。 -
Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine
結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。 -
After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。 -
2. the feature of geochemical primary halo in longshujiao ore segment shows that there is a positive abnormality of fi set of main chemical elements at 96 ~ 100 investigating line, longshujiao fault and fault 11 - 29, a east - north a positive abnormality of fs set of main chemical elements around 98 investigating line, big area positive abnormality of f4 set of main chemical elements in east ore segment and the feature of this abnormality is like the shape of the main ore - body in the west
2 、龍樹腳礦段構造原生暈特徵分析顯示， f _ 1主元素因子組在96 100勘探線，龍樹腳斷裂至? 29號斷裂間出現一個因子得分高值異常； f _ 5因子組以98勘探線為中點，因子得分值形成一個北東向高值異常； f _ 4因子組元素因子得分在東礦段形成大面積異常區，異常特徵與西部主礦體地表展布相似。 -
Argentophit cell can be found in each segment of the stomach and intestine, among them, the most positive cells were observed in the pyloric gland, the second is fundic gland of stomach
消化道各段都有嗜銀細胞，其中在最多幽門部，其次是胃底腺，嗜銀細胞以開放型為主。 -
Arginine rich human histone h3 is a kind of basic nucleosome protein. in the medium of physiological condition, by electrostatic interaction, histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge. in this paper, in order to establish a new technology of transfection, the functional domains la and ii gene segments of pea were lined with histone h3 gene segment, then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis
本實驗在已成功獲得的pea功能區a ，功能區基因片段與人組蛋白h3基因片段的克隆菌株的基礎上，構建原核表達載體，將克隆質粒pmd - 18t - pea經nco ， mlu雙酶切，回收酶切產物；將克隆質粒pmd - 18t - h3經mlu ， xho雙酶切，回收酶切產物。 -
With 2008 beijing olympic games ’ successful application and the positive preparation, china is urging to strengthen the understanding and research to the significant sport events. in tourism research, the sport event is regarded as one kind of tourism attraction. tourism research on the sport events should emphasize on tourist attendance and the number of times, and segment event tourism market, including analysis and determination on which person will implement event travel, which potential people can leave the family but to be attracted to participate the event. in order to fundamentally solve the above problems, this paper, from the prospective of travel motivation and constraint, analyzes and discusses the response which people make to the large - scale sport events ( 2008 beijing olympic games )
在旅遊研究中，體育事件是被作為一種旅遊吸引物來看待的。體育事件旅遊研究的重點應放在遊客的參與人數和次數方面，並對事件市場進行細分，包括分析和確定什麼人將進行事件旅行、哪些人可能會離開家而被吸引前來參與事件。為了從根本上解決上述問題，本文從旅遊動機和影響旅遊者旅行的限制因素方面來分析和研究了人們對于大型體育事件所做出的反應。
分享友人