procaryotic 中文意思是什麼
procaryotic
解釋
原核生物的-
This comparison uses the following model of procaryotic transcription.
下面以原核細胞轉錄作用為模式進行比較。 -
Procaryotic, expression of human heat shock protein 70 gene
人熱休克蛋白70基因的原核表達 -
The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e. coli bl21 ( de3 ). the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna編碼區序列被克隆進原核表達載體pet - 30a ，並在大腸桿菌bl21 （ de3 ）中誘導表達，但過量表達的蛋白主要是以不溶性蛋白形式存在。 -
Through these experiments we realized cloning of the gene of rbp and its expression in procaryotic cell
Colidh5中獲得了有效表達，為以後的研究工作奠定了基礎。 -
The object of this study is halr, focus on its molecular cloning, construction of eucaryote expression vector and procaryotic expression
本課題選用halr作為研究對象，重點研究其分子克隆、真核表達載體構建及原核表達。 -
Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ), then subcloned into the eucaryote expression vector pcdna3. 1 ( + ) after sequence analysis
將halr基因克隆到原核融合表達載體pet28a （ + ）上，序列分析后亞克隆到真核表達載體pcdan3 . 1 （ + ）上。 -
We integrated dh gene into procaryotic expression vector for the first time, which establish foundation for obtaining dh recombinant protein to research the structure and function of dh
本研究首次將dh基因整合入原核表達載體中表達，為大量獲取dh重組蛋白，研究dh的結構及功能奠定了基礎。 -
Procaryotic expression vector recombinant was transformed into competent bl21, induced and expressed by iptg revulsant, explored the best inducing time and the best concentration of revulsant
將原核表達載體的重組質粒轉化大腸桿菌bl21 （ de3 ） ，用iptg誘導物進行誘導表達，探索最佳誘導時間和誘導物的使用濃度。 -
These results suggested that axud1 protein functioned in a different way in the expression of cell cycle and apoptosis related proteins induced by tgf - 1 in tumor cells. 5. axudl cdna fragment was cloned into procaryotic expression vector pqe30 and t
5 . axudlcdna片段成功地克隆入原核表達載體pqe30 ，並用iptg誘導出axudi融合蛋白的表達，這為進一步純化axudi蛋白，研究axudi蛋白的免疫組織化學定位和結構功能奠定了基礎。 -
On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction
本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
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