restriction digest 中文意思是什麼

restriction digest 解釋
限制酶切消化
  • restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
  • digest : vt 1 消化，助消化。2 玩味，領會，體會（文意）。3 容忍，忍受，甘受（侮辱等）。4 （系統地）整理；匯...
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. Phaa, phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease. the expression vectors of pbv - a, pbv - b and pbv - c were constructed by orientaional cloning. indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs

    將phaa 、 phab和phac片段雙酶切后，定向克隆至原核表達載體pbv220 ，構建了三個原核表達載體pbv - a 、 pbv - b和pbv - c ；經酶切分析表明，所克隆的三個基因phaa 、 phab和phac置於表達載體的正確閱讀框架下。
  3. Cloning the gene into a pgex expression vector clone the hbrp gene using pcr, restriction digest the pgex - 5x - 1 vectors and pcr product with bamh i and xho i, recovery the pcr product and pgex - 5x - 1 vectors

    純化樣品樣品上柱， pbs洗柱后，用洗脫buf企r將蛋白洗脫下來。利用蛋白收集器，收集蛋白，濃縮結果1
  4. 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector. it was identified by restriction endonuclease digest analysis, pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene

    擴增產物連接到pgem - teasy載體上，轉化入大腸桿菌jm109中進行藍白斑篩選后，用酶切、 pcr鑒定和測序的方法鑒定出重組陽性質粒（ pgem - hc和pgem - ha ） 。
分享友人
分享到 Line

分享到臉書