restriction site 中文意思是什麼

restriction site 解釋
限制位置
  • restriction : n. 1. 限制,限定。2. 拘束,束縛;自製。3. 【邏輯學】限定。
  • site : n 1 地點;位置;地基。2 場所,現場。3 遺址。4 【計算機】網站,站點〈電腦網路用戶的網站地址〉;萬...
  1. The research showed that combined modelling technology on dynamic behaviour can take full advantage of theoretical analysis and site testing. thus the synthesis response of dynamic behaviour of the whole structure can be acquired, and the specific condition such as component joint, restriction, and damage in the model can be reflected. so this method is reliable and effective

    揚州大學碩士學位論文研究表明,動力特性聯合建模技術充分利用理論和實測的優勢,既可以獲得整體結構動力特性的綜合響應,又可以反映結構的各部件連接、約束、損傷等具體情況,是一種可靠而有效的方法。
  2. Except for the new hung hom bay reclamation, there has so far been no statutory planning restriction on the heights of residential buildings in the hung hom area, including the site of harbourfront landmark

    除了紅?灣新填海區外,紅?地區,包括「海名軒」所在地段,到目前為止並沒有對住宅樓宇高度作出法定的規劃限制。
  3. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補,進行引物延伸合成epo信號肽序列:信號肽和hsblys基因採用重疊延伸拼接法形成融合基因;融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體:引物延伸合成信號肽時,利用亮氨酸同義密碼,將信號肽基因的倒數第二個密碼突變,在重組載體上的信號肽序列之後,形成bln酶切位點,使三種載體成為分泌表達載體。
  4. The use of the facilities along the above waterfront promenade by the public in the future have to give way to the operation of the military dock, and whether the view of the harbour will be blocked by structures of the military dock ; if the view will be blocked, of the building height restriction for the site ; whether the periphery of the military dock will be designated as a closed area ; if so, whether the closed area will impede the use of facilities along the waterfront promenade by the public ; and

    將來市民使用上述海濱長廊的設施時,是不是需要遷就軍事碼頭的運作,以及碼頭建築物會不會阻擋維港景觀如果景觀會受阻,該地段的建築物高度限制是甚麼該碼頭四周會不會設置禁區如果會,該禁區會不會妨礙市民使用海濱長廊的設施及
  5. They inhibits the growth of fungi ( filamentous fungi especially ) while are non - toxic to plant cells. the main results were as follows : 1. obtaining of spcema ( signal peptide modified cema ) spcema ( 187bp ) was amplified with two long complementary primers ( p2 and p3 ) and two primers ( pl and p4 ) containing restriction enzyme recognition site

    帶信號肽cema基因的pcr合成以兩條部分重疊長鏈引物p _ 2和p _ 3延伸產物為模板, p _ 1和p _ 4為正、反引物進行pcr擴增,獲得了改造的抗菌肽基因spcema ( 187bp ) 。
  6. The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products. when there were two dna fragments of 363bp and 305bp were produced from a pcr product, the strains were assumed to have a mutation at ser83, when three fragments of 363bp, 206bp and 99bp were produced, the strains were assumed to have no mutation at the 83or 116. the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp

    本研究重點分析了16株菌對氟喹諾酮類( fqns )藥物的耐藥性,結果表明: 16株菌對諾氟沙星、環丙沙星、沙拉沙星、單諾沙星和氧氟沙星等( nor 、 cip 、 sar 、 dan 、 ofl )的耐藥率在31 . 3 - 56 . 3之間。
  7. To understand the basic theory and practice method on manufactory working site management, break through the restriction of manufactory management, implement the lean production theory and production maintenance management method, realize effective production plan and kanban management

    了解生產現場管理的基本理論和實踐方式,突破生產管理中的約束點,推行精益生產理念和全面生產維護管理方式,實現有效的生產計劃規劃和看板管理模式
  8. 2. ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220. construction of non - fusion expression plasmid pbv220 - endostatin

    用ecori和bamhi酶切endostatincdna的pcr產物,將其插入到質粒載體pbv220中相應的限制性酶切位點,構建非融合質粒表達載體pbv220 - endostatin 。
  9. The point mutation in vp2 residue 297 of canine parvovirus from ser to ala acid was examined, which causes a alteration of the restriction enzyme aiu i site

    根據vp2位點297的改變引起限制性內切酶aiu酶切位點的變化,建立pcr依賴性限制性片段長度多態性分析( prfl )方法,檢測vp2位點297的變異。
  10. " maximum number of site subscriptions that can be installed ( zero disables restriction )

    」能夠安裝的站點預定的最大數目(零表示禁用限制) 」
  11. 374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis. a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources

    通過dnastar軟體對f基因47 470nt間片段進行同源性分析比較並繪制了遺傳進化樹枝狀結構發生圖,結合334 1672nt間三種限制性內切酶( re : hinf , bsto及rsa )位點的分佈情況,確定了這些分離株的基因分類地位。
  12. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象,選用真核表達質粒pci - neo ,根據已構建好的含54位密碼子突變型mbl基因t載體的結構,設計合成新的引物, pcr擴增54位密碼突變型mbl基因,凝膠回收,雙酶切pcr產物和pci - neo質粒, t4連接酶連接,將前者克隆至後者的sma和sal位點,轉化大腸桿菌xl - 1blue ,氨芐選擇培養。
  13. Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site, and the igf - i product of pcr contains 230 base pairs. igf - ii contains 219 base pairs. 3

    各另外設計一對特異性pcr引物,導入適當限制性內切酶切點,以上述連有目的基因的克隆載體為模板,採用pcr方法擴增基因片段,獲得長度約230bp的igf -和219bp的igf -成熟肽基因序列。
  14. 6. the restriction analysis of its amplified product showed that no restriction site was observed for bamh i in all isolates, the other tested enzymes ( alu, hae iii, hinf i, taq i, hha i, msp i ) could distinguish p. cilrinopileatus from the other pleurotus species

    6 . [ ts擴增產物的限制性酶切分析結果表明,召til ) , hl對所有供試菌株均無酶切位點,而另外6利,供試內切酶( alul 、 haeitl 、 llllal 、 11infl 、九式斗, i 、 tcl叮i )都能將金頂側耳與其它側耳菌株區分開。
  15. Ba - dfe mature peptide - coding sequence was cloned to the restriction site ncol and ndei of the expression vector pet - 15b, respectively, and highly expressed in e. coli bl21 ( de3 ) by the form of non - fusion and his - badfe fusion protein

    Lmmol liptg誘導時,融合表達時產生分子量約為30kd 、帶有his標簽的ba dfe融合蛋白,非融合表達時則產生分子量為28kd的ba dfe 。
  16. The restriction map of the plasmid pbl29 was tentatively constructed according as the molecular weights of fragments of plasmid pbl29 digested with different enzymes. analysing the sequence of the plasmid pbl29 tested, we found that the mol ecular weight of the plasmid pbl29 is 371 lbp, that it s many unique restriction endonucleases, km resistance genes and abundant a / t base pairs of replicating site are on the plasmid pbl29

    同時根據限制性酶切各片段的分子量作出了質粒pbl29的內切酶圖譜。並對質粒pbl29進行測序和分析,證明了質粒pbl29大小為3711bp ,具有大量的單酶切位點、編碼卡那黴素抗性基因以及富含a t序列的復制起點。
  17. All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )

    所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法( pcr - rflp )對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型,該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換,使蘇氨酸( thr , acc )變為絲氨酸( ser , agc ) 。
  18. The pstn has some advantages, for instance, wide distribution, no restriction from time and site, no extra investment, easy prevalence, etc. the image transferring system based on pstn adequately uses existing resources with good expectation in the market

    由於pstn網路具有分佈廣泛,不受時間和地點限制,無需額外投資,易於普及等諸多優點。基於pstn的圖像傳輸系統充分利用了現有的資源,有很強的實用性,具有廣闊的市場應用前景。
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