selected strains 中文意思是什麼

selected strains 解釋
入選品系
  1. The certified strains have been selected for their tillering capacity.

    注冊品種因其分蘗能力選定。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. In this paper, genetic transformation systems by agrobacterhim rhizogems was established and optimized and high quality regenerating system was selected. the products of indigotin and indirubin in regenerating plantlets had been detected by hplc. the main studies and results were reported as follows : 1 establishment and selection of hairy root clones taken cotyledons of autotetraploid indigoblue woad as explants and hairy roots induced successfully from them by agrobaterium rhizogenes strains a4 and r1000

    本文利用發根農桿菌rit - dna建立並優化了四倍體菘藍的遺傳轉化體系，並對再生植株的主要代謝產物靛藍、靛玉紅進行了含量檢測，篩選了優良無性系，也為實現外源抗蟲、抗病等基因的導入奠定了基礎。
  4. In this paper, thirty strains of yeast were isolated from nineteen samples of fermented milk ( ten koumiss samples, three fermented goat milk samples, four fermented milk samples, two shubat samples ) which were collected from the west - centre region of inner mongolia from which we aimed to select the yeast with high vitalistic ? galactosidase. according to the results of dedermination that were the metabolism rate and ratio vitality of ? galactosidase, stk - 1 - 1 has been selected. the result of identification showed that stk - 1 - 1 belonged to kluyveromyces marxianus of kluyveromyces

    本研究主要以內蒙古中西部地區牧區採集的19個發酵乳樣品（ 10份酸馬奶、 3份發酵山羊奶、 4份酸牛奶和2份發酵駝奶）為研究對象，分離獲得30株酵母菌，從中篩選乳糖酶高活力酵母菌株。根據乳糖代謝率和乳糖酶比活力的測定結果，選出菌株stk - 1 - 1為優勝菌株。經鑒定，該菌株屬克魯維酵母屬（ kluyveromyces ）的馬克斯克魯維酵母（ kluyveromycesmarxianus ） 。
  5. On the basis of the separation and identification of streptococcosis suis in the southern part of henan province, the certain typical bacterial strains were selected as the vaccine strains to produce polyvalent inactivity vaccine for the local prevention of the disease

    摘要在對豫南地區豬鏈球菌病病原分群鑒定的基礎上，挑選具有一定代表性的菌株作為疫苗株，研製多價滅活疫苗免疫防制豬鏈球菌病。
  6. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  7. In this study a microbial system for biphenyl biodegradation is set up in order to investigate the effects of surfactants on biodegradation of hydrophobic organic compounds. four strains which can grow on biphenyl as the sole carbon and energy sources are selected out, in which alcaligenes eutrophus dj1 and pseudomonas sms02 are chosen as degradation strains. after studing the bioavailability of three nonionic surfactants ( op - 10, tween - 80, and triton x - 100 ), they are added into the biodegradation system of biphenyl

    為了考察表面活性劑對疏水性有機污染物生物降解的影響，本論文選擇聯苯作為模擬研究體系，篩選、馴化得到四株可利用聯苯作為碳源生長的菌株；考察了聯苯降解菌株對三種非離子表面活性劑op - 10 、 tween - 80 、 tritonx - 100的生物可利用性；用高效液相色譜法測定了非離子表面活性劑對聯苯的增溶曲線；較系統地研究了加入非離子表面活性劑對聯苯生物降解速率的影響。
  8. In six selected areas of the subtropical semi - humid evergreen broad - leaf forest ecosystem of yunnan diaolin mountain, during drought season from november, 1992 to april, 1993, authors studied and analysed, throught adopting the taxonomic, ecological and mathematical statistics method, the distribution, composition and domi - nant fungi of the small fungi as well as biodiversity. altogether 706 strains statistics unit, belonging to 28 genera, dominant fungi 678 strains statistics unit, 20 dominant fungi gen - era

    在雲南雕林山亞熱帶半濕潤常綠闊葉林森林生態系統的六個具有代表性的樣區中，於1992年11月至1993年4月旱季期間，運用系統分類、生態分析和數理統計的方法，對小型真菌種群的分佈、組成、優勢菌及其生物多樣性進行了分析，共分離獲得小型真菌菌株統計單位706株，分屬於28個屬，其中，優勢菌678株，分屬於20個屬。
  9. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  10. After enriching, separating, first - screening and second - screening, induced mutation and screening after induced mutation, five strains capable of producing high fucoidanase activities are separated and selected

    經富集，初篩，復篩，誘變，誘變后篩選，得到5株產酶高活力的菌株。其中菌株a活力最高，酶活達到3 . 68u 。
  11. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  12. In this thesis, a strain of associative nitrogen fixing bacteria with high nitrogenase activity was selected and the further studies for it were conducted, the results obtained are as follows : selection and identification of associative nitrogen fixing bacteria with high nitrogenase activity. the nitrogenase activities detected by means of the acetylene reduction method and growth rates tested by optical density of germ suspension under the x 400nm ( od4oo ) of all the strains tested except for the strain w12 reduced gradually due to frequent subculture during a year. however, the strain w12 has been showing high and stable nitrogenase activity and growth rate since it was isolated five years ago

    固氮菌株的篩選與鑒定：測定從不同來源分離、收集和保藏的12個固氮菌株或分離物在不同保存時期的固氮酶活性和生長能力，發現多數菌株在初分離得到時固氮酶活性較高，但隨著轉接次數的增多卻逐漸喪失固氮酶活性，生長也隨之減弱，只有w12在五年前從埃及分離得到至今一直保持旺盛生長勢和較高的固氮酶活性， 48h內乙烯生成量可達1800nmol乙烯ml菌液以上，遠遠高於其它菌株，其生長量（ od _ （ 400 ）值）也遠遠高於其它菌株。
  13. A screening method that allows available, efficient and reliable selection of hydantoinase - positive micro - organisms was devised. the soil samples were collected from different region, which were enriched by using 5 - phenylhydantoin as the unique nitrogen. the first detection was through screening plates, the 23 candidate strains were further selected by detection of the medial product and final product

    2 .建立了一個可行的篩選模型，從不同區域採集土樣，以5一苯基乙內酞服為唯一氮源進行土樣富集，通過平板篩選法進行初篩后，得到23株候選菌株，通過檢測中間產物和終產物的產生進行復篩，結果初步確定了1株乙內酞脈酶產酶菌株，編號為bts11 。
  14. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  15. Part 1 study on the antibiotic resistance of escherichia coil and klebsieua pneumoniae producing extended - spectrum beta - lactamases objective to investigate the prevalence of strains producing esbls among escherichia coil and klebsieua pneumoniae, look for the best detection substrate of these strains and determine the antibiotic resistance of them. methods 248 strains of e. coli. and 97 strains of k. pn from january to october, 2002 were investigated for production of extended - spectrum b - lactamases ( esbls ) by phenotypic screening and confirmatory test provided by the national committee for clinical laboratory standards ; compared the result of the phenotypic screening and confirmatory test to discuss the best detection substrate of these strains ; 16 kind of antibiotics were selected for antimicrobial susceptibility test for the resistance

    方法對2002年1 ? 10月臨床分離的248株e . coli和97株k . pn ，採用美國臨床實驗室標準委員會（ nccls ）規定的esbls表型篩選和確證試驗，確定本地區esbls的發生率；通過對不同底物的初篩結果與確證試驗結果的比較，探討本地區臨床檢測產esbls菌株的最佳篩選底物；選擇16種抗生素紙片做藥敏試驗，了解產esbls的e . coli和k . pn的耐藥性。
  16. The strains with high expression level is selected according to the result of expression experiment with the methods introduced by expression guideline, and its expression condition is op timized in three aspects, namely time of inducing, ph value and inducing concentration of methanol

    按表達手冊進行表達實驗，挑選出具有高分泌表達水平的菌株，並採用不同的誘導時間、 ph值、甲醇誘導濃度進行表達比較，優化出適宜的表達條件。
  17. Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis. so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library. cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids

    以此片段為探針釣出了位於該區域的文庫克隆，並利用指紋印跡和雜交技術將這些文庫克隆排列起來，進而以位於該區域的不同位置的片段做臂構建了用於轉化和接合轉移用的基因置換質粒，並試圖通過基因置換將該區域置換下來，但尚未得到最終結果。
  18. Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe. wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ), and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution

    該純化cpe對小白鼠的半數致死量為2 . 5ug /只；該cpe豚鼠皮膚藍斑單位為2500 4000 ；引起紐西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ，在含0 . 4福爾馬林的的pb液中透析制備類毒素。
  19. Respectively. laboratory tests on phosphorus release abilities of selected bacterial strains showed ipb could liberate more than 50 g / ml, while opb produced less than 2 g / ml water soluble inorganic phosphorus ( wsip ) every day, demonstrating the enzymes participating in solubilization of ca - p should be constitutive ferments and enzymes concerning with mineralization of lecithin might be inducible catalysts. low abundance and poor compositions of nitrogen utilizing bacteria suggested poor bacterial diversities and weak self - purification of the lake

    對3株有代表性的無機磷分解菌和6株占優勢的有機磷分解菌的鑒定顯示， 3株無機磷分解菌與巨大芽孢桿菌、大地桿菌和janthinobacteriumlividum最相近，而6株有機磷分解菌分別為固氮螺菌，產氣腸桿菌， blastobacteraggregatus ，表皮葡萄球菌，溶血不動桿菌和念珠菌。
  20. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下： 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板培養基從土樣中篩選出102株酸性植酸酶產生菌，從中挑選出透明圈較大的菌株32株，經分離純化後分別進行搖瓶復篩， 28 、 220r / min發酵5天後，在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活，結果發現有3株菌產酶活性較高且產酶性能較為穩定。
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