serum-free culture 中文意思是什麼
serum-free culture
解釋
無血清培養-
Using the mouse fetal ovary serum - free culture model, fetal ovaries from 14 day post coitus ( 14 dpc ) mouse were cultured, and treated by ay9944 - a - 7, nystatin and rs - 21745. the results showed that 0. 025, 0. 0625 and 0. 125 um ay9944 - a - 7 or 25, 50 and 75 iu / ml nystatin increased the total number of follicles per ovary significantly ; however, ay9944 - a - 7 and nystatin at the same doses could n ' t cause the same effect on the number of growing follicles and the average diameter of five largest follicles per ovary. 50 u. m rs - 21745 decreased the total number of follicles, the number of growing follicles and diameter of follicles per ovary significantly after 48 h
首先利用小鼠胚胎卵巢的體外無血清培養模型，培養妊娠14天（ 14daypost - coitus ， 14dpc ）小鼠胚胎卵巢，分別添加能促進mas積累的ay9944 ，制黴菌素，和能抑制mas產生的rs - 21745進行處理，結果表明： 0 . 025 、 0 . 0625利0 . 125 m的ay9944 - a - 7與25 、 50和75iu ml的制黴菌素能顯著提高卵巢中形成卵泡的總數量，但是對生長卵泡數和卵泡直徑的作用不同；而mas合成抑制劑rs - 21745能夠顯著降低形成卵泡的總數量。 -
Methods : cell culture in serum - free medium, indirect immunofluorescence cytochemistry were used
方法：採用無血清細胞培養技術，間接免疫細胞化學染色法。 -
Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture
提要：採用無血清無動物組分培養基馴化高效表達細胞，構建高效表達系統，懸浮培養細胞，可以在規模化生產中，提高細胞密度，增強細胞活力，控制細胞增殖水平，延長細胞培養周期，增加目標蛋白的產量，提高產品質量，簡化生產工藝，降低生產成本，進而提高規模化培養的效能。 -
Conclusion this is a serum - free culture system for developing a conjunctival epithelial explants with improved purify and proliferative, which are important for the basic research and tissue engineer of conjunctival epithelial cells
結論無血清培養系統的組織塊培養法可為結膜上皮細胞的疾病、藥理毒理研究及培養移植的結膜上皮細胞提供更為安全有效的細胞模型。 -
Thus, the success of nscs therapy is determined by the induced differentiation and the effect of micro - enviroment on the survival, proliferation and differentiation of it. the isolation and culture of nscs in vitro successfully is the presupposition for its research. to avoid the influence of unknown factors exiting in serum on nscs " differentiation, serum - free medium with egf and bfgf as mitogens to stimulate proliferation and inhibit differentiation in vitro was introduced through a long period of search
澱粉樣蛋白（ amyloidpeptidy ， a ）是澱粉樣蛋白前體蛋白（ amyloidprecusorprotein ， app ）異常代謝產生的含有39 ? 43個氨基酸殘基的疏水肽，可聚合成不溶的纖維形式或不定型顆粒狀結構，導致各種毒性反應，在阿爾茨海默病（ alzheimer ' sdisease ， ad ）發病中起著至關重要的作用。 -
Objective to investigate the culturing technique for conjunctival epithelial cells and to evaluate the facibility of serum - free culture system on culturing conjunctival epithelial explants
摘要目的研究結膜上皮細胞體外培養的方法，並進一步探索無血清培養系統用於結膜上皮細胞培養的可行性。 -
Development of insect cell culture media and serum - free media, establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper
摘要綜述了昆蟲細胞培養基的現狀和發展、無血清培養基的開發、昆蟲細胞系的建立以及昆蟲細胞在生物農藥和重組蛋白中的應用。 -
Experimental condition optimization study on inducing mouse embryonic stem cells differentiation into insulin producing cells by serum - free culture in vitro
無血清培養法誘導小鼠胚胎幹細胞分化為胰島素分泌細胞的實驗條件優化選擇 -
To initiate induction, undifferentiated escs were dissociated and replanted on none - coated tissue culture plastic dishes in nsc medium. the medium was changed ever - y other day into serum - free nsc medium
誘導分化時去飼養層細胞，重新接種于無包被的培養皿中，使用nsc培養液，逐漸更換為無血清nsc培養液。 -
A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions, which means the cells should have some anti - apoptosis property ; if not cultured i n suspension, the cells should also be able to grow in adherence
一株成功的工程細胞除了要求目的蛋白的表達量高外，還必須適應無血清培養基培養；必須具有對不利環境的抵抗能力，即抗凋亡能力；對于非直接懸浮培養的細胞，還必須具備在無血清培養條件下的貼壁能力。 -
However, the drawbacks of mammalian cell expression systems are apparent : they are difficult to culture in large scale, especially in serum - free medium ; the productivity is much lower than other expression systems and the costs are higher
但哺乳動物細胞表達系統的缺點也很明顯，如表達量低、大規模培養困難、生產成本高昂等。
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