squalene synthase 中文意思是什麼
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For the first time a 1539bp squalene synthase ( asqs ) cdna was cloned from a high - yield artemisia annua strain 001 by race strategy
用race方法首次從青蒿中克隆了一個1539bp全長鯊烯合酶cdna 。 -
Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達，而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。
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